pJL02 vector (V015325)

LOCUS       62056_13180        9795 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL02, complete sequence.
ACCESSION   MT370814
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..32)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(36..1115)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(1116..1315)
                     /label=derived from pRpoD
                     /note="derived from pRpoD"
                     /regulatory_class="promoter"
     protein_bind    1322..1338
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1508..1531
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     primer_bind     complement(1623..1639)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2624..3436
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4253..4647)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4989..5076
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6315..7283
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7797..8633
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8623..9471
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"