LOCUS       62056_13180        9795 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL02, complete sequence.
ACCESSION   MT370814
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..32)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(36..1115)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(1116..1315)
                     /label=derived from pRpoD
                     /note="derived from pRpoD"
                     /regulatory_class="promoter"
     protein_bind    1322..1338
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1508..1531
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     primer_bind     complement(1623..1639)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2624..3436
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4253..4647)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4989..5076
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6315..7283
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7797..8633
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8623..9471
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cgagaaaagg ccatccgtca ggatggcctt cttcactgcc cgctttccag tcgggaaacc
       61 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      121 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
      181 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
      241 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
      301 cccactaccg aaatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
      361 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
      421 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
      481 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
      541 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
      601 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
      661 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
      721 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
      781 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
      841 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
      901 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
      961 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     1021 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     1081 tactctgcga catcgtataa cgttactggt ttcatctttg cttactcttt gttgaatctg
     1141 tggcagtgaa aaaccgtgca tgatagcaca atttgcttga aatgtttggg ctttgatcgg
     1201 tgaccgttaa ataaaaaata tctttgaatt cacgactatt ttttgaatat tgggctaaaa
     1261 tcattttttt caaggttaaa tcatagaaaa atttgtagaa atcttcgctt atcaattgac
     1321 attgtgagcg gataacaata taatttggaa ttgtgagcgg ataacaattt cacattggaa
     1381 ttgtgagcgg ataacaattt cacttggaat tgtgagcgga taacaattta aattggaatt
     1441 gtgagcggat aacaatttca caaattggaa ttgtgagcgg ataacaattt tccgaggact
     1501 gcccatggac tacaaagacg atgacgacaa gggatcccac cgcggtgtct agaactagtc
     1561 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacggtacc gagctcgaat
     1621 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1681 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1741 cgcccttccc aacagttgcg cagcctgaat ggcgaatgtc tggtcggtga agtgggcttt
     1801 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     1861 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     1921 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     1981 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     2041 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     2101 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccaaac aggaaacgca
     2161 gctgtaatcc gggcagcgca acggaacatt catcagtgta aaaatggaat caataaagcc
     2221 ctgcgcagcg cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc
     2281 aaggtcagaa tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
     2341 ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt
     2401 gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     2461 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca
     2521 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2581 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2641 ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2701 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     2761 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2821 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2881 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc
     2941 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3001 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3061 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3121 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
     3181 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3241 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3301 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3361 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3421 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3481 gctgacttga cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     3541 cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc
     3601 accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg
     3661 gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct
     3721 cagcgctatt ctgaccttgc catcacgact gtgctggtca ttaaacgcgt attcaggctg
     3781 accctgcgcg ctgcgcaggg ctttattgat tccattttta cactgatgaa tgttccgttg
     3841 cgctgcccgg attacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa
     3901 cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca
     3961 catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact
     4021 cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat
     4081 cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac
     4141 tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct
     4201 gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc
     4261 tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg
     4321 cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc
     4381 atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg
     4441 cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta
     4501 cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca
     4561 ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac
     4621 ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc
     4681 ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg
     4741 tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc
     4801 aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt
     4861 tcccttgccc gcacccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg
     4921 gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt
     4981 gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg
     5041 gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt
     5101 gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg
     5161 cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca
     5221 aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg
     5281 ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg
     5341 acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg
     5401 agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg
     5461 ccgagcgcct gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc
     5521 acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca
     5581 agcggtacaa cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc
     5641 ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag
     5701 agcgggctgg ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc
     5761 gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca
     5821 ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac
     5881 aggaataccg ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc
     5941 atcaacgagt tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac
     6001 gaagcccggc aggtcatgag ccagacccag caggccagcg aggcgcaggc ggcggagtgg
     6061 ctgaaagccc agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag
     6121 gtagccgccg aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac
     6181 tggaagctat ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc
     6241 gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg
     6301 cgcttggtgg cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg
     6361 ccatgggctg tgagcgcttc gatatcggcg tcagggacgc caccaccggc cagatgatga
     6421 accgggaatg gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg
     6481 cccagggcaa tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg
     6541 tggacgacct cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc
     6601 tggtagtgga aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag
     6661 gcggtgaact tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg
     6721 ccagcgccga cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc
     6781 acaccacccg cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg
     6841 ccaccgctgg cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc
     6901 agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc
     6961 accggcgcac ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg
     7021 gtgatgacct cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca
     7081 gtgccgagga aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc
     7141 ccggccacga agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg
     7201 tccagcttgc gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca
     7261 ggggcgggcc agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg
     7321 agtactcacg cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct
     7381 acctgatcaa aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca
     7441 aggccgaggc tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca
     7501 ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat
     7561 atgaaagacc aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc
     7621 caagcgcgat atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg
     7681 accgacgacg aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc
     7741 gggggtagtg accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg
     7801 ctacccataa gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact
     7861 acgttttgcc caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg
     7921 gtaaatccat gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg
     7981 aggtgggcga actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg
     8041 ccattcatca ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg
     8101 tggctgacgg cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg
     8161 agtggttcga cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc
     8221 tgcgccggtt ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc
     8281 gcatggaggc catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca
     8341 agggcgcggc catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac
     8401 tggtcgataa catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg
     8461 aatggggtgt ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca
     8521 actatggcgc accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc
     8581 ccgccgtgct ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac
     8641 aagcacagcc tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc
     8701 cgtgccctca agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc
     8761 gacggcaagc ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc
     8821 ctgcaagggc tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag
     8881 accgaaggcg gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct
     8941 gatgccatgg tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc
     9001 gatagtggtg gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc
     9061 atcatcgccc agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc
     9121 gacgaggcgg acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg
     9181 ggtggcggcc agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc
     9241 gcccgcctgc tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct
     9301 tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc
     9361 aagcgccgcc agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta
     9421 accgagttcg cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc
     9481 ccactctatt gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta
     9541 attggtaata ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt
     9601 gcctactgag cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt
     9661 atgctcttct ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9781 caggaaagaa catgt
//
