pNR28.12 vector (V015306)

LOCUS       62056_18195        8363 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Cloning vector pNR28.12, complete sequence.
ACCESSION   MH553564
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Identification of a genetic element required for spore killing in 
            Neurospora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) School of Biological Sciences, Illinois 
            State University, ISU School of Biological Sciences, Normal, IL 
            61790, USA
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2018) School of Biological Sciences, Illinois State 
            University, ISU School of Biological Sciences, Normal, IL 61790, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="histone 3"
                     /label=histone 3
                     /note="his-3"
                     /protein_id="QBA09473.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2801..3155
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3159..3725
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     CDS             3758..3778
                     /codon_start=1
                     /transl_table=11
                     /product="TrpC"
                     /label=TrpC
                     /note="A. nidulans TrpC"
                     /protein_id="QBA09472.1"
                     /translation="AAKSAF"
     regulatory      3779..4317
                     /label=Aspergillus nidulans trpC terminator region
                     /note="Aspergillus nidulans trpC terminator region"
                     /regulatory_class="terminator"
     promoter        complement(4340..4358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4360..5604
                     /note="Neurospora crassa intergenic spacer between his-3
                     and rat-1"
     CDS             complement(5717..5728)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(6003..6021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6279..6867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7041..7898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7899..8003)
                     /label=AmpR promoter