pNR28.12 vector (V015306)

MSDS

Basic Vector Information

Vector Name:: pNR28.12

Antibiotic Resistance:: Ampicillin

Length:: 8363 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Hammond TM, Rhoades NA.

Promoter:: trpC

pNR28.12 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNR28.12 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18195        8363 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Cloning vector pNR28.12, complete sequence.
ACCESSION   MH553564
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Identification of a genetic element required for spore killing in 
            Neurospora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) School of Biological Sciences, Illinois 
            State University, ISU School of Biological Sciences, Normal, IL 
            61790, USA
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2018) School of Biological Sciences, Illinois State 
            University, ISU School of Biological Sciences, Normal, IL 61790, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="histone 3"
                     /label=histone 3
                     /note="his-3"
                     /protein_id="QBA09473.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2801..3155
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3159..3725
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     CDS             3758..3778
                     /codon_start=1
                     /transl_table=11
                     /product="TrpC"
                     /label=TrpC
                     /note="A. nidulans TrpC"
                     /protein_id="QBA09472.1"
                     /translation="AAKSAF"
     regulatory      3779..4317
                     /label=Aspergillus nidulans trpC terminator region
                     /note="Aspergillus nidulans trpC terminator region"
                     /regulatory_class="terminator"
     promoter        complement(4340..4358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4360..5604
                     /note="Neurospora crassa intergenic spacer between his-3
                     and rat-1"
     CDS             complement(5717..5728)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(6003..6021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6279..6867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7041..7898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7899..8003)
                     /label=AmpR promoter

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