LOCUS       62056_18195        8363 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Cloning vector pNR28.12, complete sequence.
ACCESSION   MH553564
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Identification of a genetic element required for spore killing in 
            Neurospora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Hammond TM, Rhoades NA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) School of Biological Sciences, Illinois 
            State University, ISU School of Biological Sciences, Normal, IL 
            61790, USA
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2018) School of Biological Sciences, Illinois State 
            University, ISU School of Biological Sciences, Normal, IL 61790, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(16..1864,1928..2124)
                     /codon_start=1
                     /transl_table=11
                     /product="histone 3"
                     /label=histone 3
                     /note="his-3"
                     /protein_id="QBA09473.1"
                     /translation="KLAISTILSSVWKSDRPDGLLPTVVVDEHDTALGLVYSSAESVNE
                     ALRTQTGVYQSRKRGLWYKGATSGDTQELVRISLDCDNDALKFVVKQKGRFCHLDQSGC
                     FGQLKGLPKLEQTLISRKQSAPEGSYTARLFSDEKLVRAKIMEEAEELCTAQTPQEIAF
                     EAADLFYFALTRAVAAGVTLADIERSLDAKSWKVKRRTGDAKGKWAEKEGIKPAASALA
                     ATSAPVTKEAAQETTPEKITMRRFDASKVSTEELDAALKRPAQKSSDAIYKIIVPIIED
                     VRKNGDKAVLSYTHKFEKATSLTSPVLKAPFPKELMQLPEETIAAIDVSFENIRKFHAA
                     QKEEKPLQVETMPGVVCSRFSRPIEAVGCYIPGGTAVLPSTALMLGVPAMVAGCNKIVF
                     ASPPRADGTITPEIVHVAHKVGAESIVLAGGAQAVAAMAYGTESITKVDKILGPGNQFV
                     TAAKMFVSNDTNAAVGIDMPAGPSEVLVIADKDANPAFVASDLLSQAEHGVDSQVILIA
                     IDLDEEHLQAIEDEVHRQATELPRVQIVRGSIAHSITVQVKTVEEAMELSNKYAPEHLI
                     LQIKEAEKAVDLVMNAGSVFIGAWTPESVGDYSAGVNHSLPTYGFGKQYSGVNFASFVK
                     HITSSNLTAEGLKNVGQAVMQLAKVEELEAHRRAVSIRLEHMSKSN"
     promoter        2663..2681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2714..2730
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2764..2780)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2801..3155
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3159..3725
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     CDS             3758..3778
                     /codon_start=1
                     /transl_table=11
                     /product="TrpC"
                     /label=TrpC
                     /note="A. nidulans TrpC"
                     /protein_id="QBA09472.1"
                     /translation="AAKSAF"
     regulatory      3779..4317
                     /label=Aspergillus nidulans trpC terminator region
                     /note="Aspergillus nidulans trpC terminator region"
                     /regulatory_class="terminator"
     promoter        complement(4340..4358)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4360..5604
                     /note="Neurospora crassa intergenic spacer between his-3
                     and rat-1"
     CDS             complement(5717..5728)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(6003..6021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6279..6867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7041..7898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7899..8003)
                     /label=AmpR promoter
ORIGIN
        1 gaactcgagc agctgaagct tgccatctcc accatcctct cgagcgtctg gaagtctgac
       61 cgtcccgatg gtcttctccc caccgttgtc gttgatgagc acgacactgc tctgggtctg
      121 gtctacagca gtgccgagag tgtgaacgag gccctcagga cacagactgg tgtctatcag
      181 agccggaagc gcggtctctg gtacaagggt gctacttccg gagacactca ggagctcgtc
      241 cgcatctcgc ttgactgcga taacgatgct ctcaagtttg tcgtgaagca gaagggtcgt
      301 ttctgccacc tcgatcagtc cggctgcttt ggtcagctca aaggccttcc caagctcgag
      361 cagactttga tttcgaggaa acagtctgcc cccgagggct cctacactgc ccgtctcttc
      421 tccgatgaga agctagtccg ggccaagatc atggaggagg ctgaggagct ctgcaccgct
      481 cagacccccc aggaaatcgc ctttgaggct gccgatctct tctactttgc tcttaccagg
      541 gccgttgctg ccggcgttac tcttgccgat atcgaaagga gccttgacgc caagagctgg
      601 aaggtcaagc gcaggactgg agatgctaag ggtaagtggg ctgagaagga gggcatcaag
      661 cctgcggcgt ccgctctcgc tgccacttcg gcccctgtca ccaaggaggc cgcccaggag
      721 accacccctg agaagatcac catgagacgt ttcgacgcct ccaaggtctc taccgaggag
      781 ctcgatgctg ctctcaagcg tcctgcgcaa aagtcgtccg atgccatcta caagatcatt
      841 gtccccatca tcgaggacgt ccgcaagaac ggcgacaagg ctgttctgtc gtacactcac
      901 aagttcgaga aggctacctc tcttactagc cccgtcctga aggcgccctt ccccaaggag
      961 cttatgcagc tccctgagga gaccattgct gccatcgacg tgtccttcga gaacatccgc
     1021 aagttccacg ccgcccagaa ggaggagaag cccctccagg tcgagaccat gcccggtgtt
     1081 gtctgcagcc gtttctctcg tcccatcgag gccgtcggct gctacatccc cggcggtacc
     1141 gccgttctcc ccagcactgc ccttatgctg ggtgttcccg ccatggtcgc cggctgcaac
     1201 aagattgtgt tcgcctctcc tccccgcgcc gacggaacca tcactcccga gattgtccac
     1261 gtcgctcaca aggttggggc cgagtccatc gtgcttgccg gcggtgccca ggccgtagct
     1321 gccatggcct acggcaccga gagcatcacc aaggtcgaca agattctcgg ccccggtaac
     1381 cagttcgtca ctgctgccaa gatgttcgtc agcaacgaca ccaacgctgc cgttggtatt
     1441 gacatgcccg ctggcccgtc cgaggtgctg gtcatcgctg acaaggacgc caaccccgcg
     1501 ttcgttgcct cggatctcct gtcccaggct gagcacggcg ttgacagtca ggtcatcctg
     1561 atcgctattg acctcgacga ggagcatctt caggctattg aggacgaggt tcaccgtcag
     1621 gctacggagc ttcctcgcgt ccagattgtc cgtggctcca tcgcccactc gatcaccgtg
     1681 caggtcaaga ccgtcgagga ggccatggag ctcagcaaca agtacgctcc tgagcacttg
     1741 atcctccaga tcaaggaggc cgagaaggct gtcgatcttg tcatgaacgc cggtagtgtc
     1801 ttcattggcg cctggactcc tgagtccgtt ggcgattact ctgctggtgt taaccactcg
     1861 ctgcgtaagt tacatatcat aaatagcccc gcttcacaga ttcttctgct aacgtcaaga
     1921 cacatagcta cctatggctt tggcaagcag tactctggcg tcaatttcgc ctcgttcgtc
     1981 aagcacatta ccagctccaa cttgactgcc gagggtctca aaaacgtcgg ccaggctgtc
     2041 atgcagttgg ctaaggttga ggagctcgag gctcacagaa gggcggtcag catccgtctt
     2101 gagcacatga gcaagagcaa ctaaacggaa attcttttcg aagtagcaaa aaaaaaaaaa
     2161 aaaaacaaga acaaaaggat gtagtgggtt gatgtatatc tgggtcattt tgggcacata
     2221 gagtaatgat aacgagtttt ggacattgta ctgttctgta caggctgaag atcagtacat
     2281 gaatctgttg gtaagtgtgg agacccaaac ggcccttgag tttttctccc tattccagca
     2341 gatgaggtgc tcgtccctgg gtgtttattt tcattattac atcaaccttt tttttttttt
     2401 tttttttcag atcatgcgta catgaacggg ggaagcacag acgatcgaaa cgtggatgtc
     2461 acaatgtccc tgcagtgatg ctgcattgca tgaagcgccc atctcaaaaa acttgcagtc
     2521 ttgcacgttg catgtgaacc tcccaaacaa ccgaataaaa gacggcgaaa aatgaagata
     2581 aaaaaaaacc ataaaaaaaa tcagagggag tgtgggaaat ggtgtctttt agcattcaga
     2641 ccccattagc cgtccacgcc cttaatacga ctcactatag ggcgaattgg agctccaccg
     2701 cggtggcggc cgctctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
     2761 tatcgatacc gtcgacctcg agggggggcc ctgcagataa ctgatattga aggagcattt
     2821 tttgggcttg gctggagcta gtggaggtca acaatgaatg cctattttgg tttagtcgtc
     2881 caggcggtga gcacaaaatt tgtgtcgttt gacaagatgg ttcatttagg caactggtca
     2941 gatcagcccc acttgtagca gtagcggcgg cgctcgaagt gtgactctta ttagcagaca
     3001 ggaacgagga cattattatc atctgctgct tggtgcacga taacttggtg cgtttgtcaa
     3061 gcaaggtaag tggacgaccc ggtcatacct tcttaagttc gcccttcctc cctttatttc
     3121 agattcaatc tgacttacct attctaccca agcatcccat ggccaccctc gacgacacgg
     3181 cttaccggta ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt
     3241 ccttcaccac cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg
     3301 aggtgccggt ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat
     3361 cggacgacgg ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg
     3421 gcgacctggc gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg
     3481 aggacatcga ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc
     3541 tcgcgacgga gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg
     3601 tcaacgcacc ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca
     3661 ccgccctgta cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct
     3721 gcccctgatc tatctttcta gagagtgcct tctagtggca gcaaagagtg ccttctagtg
     3781 atttaatagc tccatgtcaa caagaataaa acgcgtttcg ggtttacctc ttccagatac
     3841 agctcatctg caatgcatta atgcattgga cctcgcaacc ctagtacgcc cttcaggctc
     3901 cggcgaagca gaagaatagc ttagcagagt ctattttcat tttcgggaga cgagatcaac
     3961 agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc tccacgcgac
     4021 tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg atagcttgac
     4081 tatgaaaatt ccgtcaccag cccctgggtt cgcaaagata attgcactgt ttcttccttg
     4141 aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa atcattccta
     4201 ctaagatggg tatacaatag taaccatggt tgcctagtga atgctccgta acacccaata
     4261 cgccggccga aactttttta caactctcct atgagtcgtt tacccagaat gcacagggcc
     4321 cggtacccag cttttgttcc ctttagtgag ggttaattag ggccaagcta ccccgtcaaa
     4381 tctatacata tctataacgg gtttcagggg tttcgttcgc tgtcaatcaa gtttgaaaca
     4441 tcactggggc cgttggacgg tgtattagac cattggctcc ctcagctgtt tggcggctgg
     4501 gcggctgggt caaacggcaa taacgggact cgagagggac gaggagagtc ggttggctgg
     4561 ctgcaataca agcgttccca cctaaccaac gagtcccgtt ttccatttgt gtgcctaacc
     4621 atcatctagg gatgtcaggg tttggccgga tcagggtatg tttggttgac tgttgtcatg
     4681 tctgattggg tacatattat ggtaggtgtc tcgagaacag tagagtactc gggcctagcg
     4741 tttggatgat tacgcgagat atgagttgtg ggccgccatg cagttgcttg tccataagca
     4801 gaagttgctt tgggatatat ttctcgtctt tcaaaggtca cgaggtcctg ggacgaacgg
     4861 catcgccatc caaagggttg aacatgagaa acctgaatgg cctttgcgtt gaaatacaaa
     4921 aagtcaagaa caaaatcgct tgaggatagg gacgtggaag caagcaaata tggtaagaga
     4981 ggtatacatc aaccctggtt caattgttag cgtggttctt cctccacgtc ctcgttcatg
     5041 acggttaaca gtaccaggct aacaattaaa ccagggttga tgtgtactga tatgtaggtg
     5101 ctcagcaaac tgccaatttc ttggccccaa gcagcagttt gctgtcagtg ctgctcgtgt
     5161 cagccttggt agtggaacct aaactgctaa cacagcgcaa gtgcgcatgt aaagatattg
     5221 tgggaggatc tgtatggatg gatgagatta ctgcttggtg ttggttgcga ggcactgcgg
     5281 ctgttaggct ttgctgtgcc ccgttcgacg aagaaatacg cggaactata aattggatac
     5341 ctagacttac tgcctatggg aggtatctac cgacgtagcc gacggattct agcaacatcc
     5401 cgactttgct tgtagtgtac tatgatagca gcacagtgtt gctccttgtg agaatgggct
     5461 cttttttttt ttcccccttc cctagggcgt tgactggact tgctctattg ttcccaaggt
     5521 aggtgcccgt catcgatttt cccaagtctc ccgccagatt gtcgtcatag tgtcatgatg
     5581 acctcggtcg ctggggctgc gtggttacgg ggagctggga ccgctaggcc tacagtggtt
     5641 gtgccattca gcgtgggtgt gtggagtagc ggtagaggcg cttggaagtt gtgctagcgg
     5701 aaaccctgga atatcttcta ccctcgattc cttctcgggc tgcccatgtg ctgaggtgat
     5761 gccggggatc tggcgccaat catccattga ggttcccgca gcttcccggt gccgcgcgcg
     5821 ggcgcagttg ctcacaggac acacctagac gcaggggcac aggggcaccg tttggtgtgc
     5881 aactgggtac ctagctgtag caagcactcc accgtctgtg caatccccca atccacggca
     5941 ggaacttcgc accgccgcgg caccgagtga gcgaatccat ccgcattgga tctgccggtc
     6001 tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc
     6061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     6481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8041 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     8101 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8161 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8221 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     8281 catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
     8341 cacatacgat ttaggtgaca cta
//
