pCW154-E6E7 vector (V015265)

LOCUS       V015265                 9525 bp    DNA     circular SYN 06-JUL-2021
DEFINITION  Exported.
ACCESSION   V015265
VERSION     V015265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Wang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2020) Sichuan University, West China School of
            Public Health, Renmin Nan Road, Chengdu, Sichuan 610041, China
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2020) Sichuan University, West China School of Public
            Health, Renmin Nan Road, Chengdu, Sichuan 610041, China"
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1590..2321)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             4135..4161
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5125..5157
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     promoter        6668..6772
                     /label="AmpR promoter"
     CDS             6773..7630
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7804..8392
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8578..8718)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     primer_bind     8804..8826
                     /label="pGEX_3 primer"
                     /note="pGEX_3 primer"
     CDS             complement(8823..9011)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"