LOCUS       V015265                 9525 bp    DNA     circular SYN 06-JUL-2021
DEFINITION  Exported.
ACCESSION   V015265
VERSION     V015265
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Wang C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2020) Sichuan University, West China School of
            Public Health, Renmin Nan Road, Chengdu, Sichuan 610041, China
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2020) Sichuan University, West China School of Public
            Health, Renmin Nan Road, Chengdu, Sichuan 610041, China"
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1590..2321)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             4135..4161
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5125..5157
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     promoter        6668..6772
                     /label="AmpR promoter"
     CDS             6773..7630
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7804..8392
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8578..8718)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     primer_bind     8804..8826
                     /label="pGEX_3 primer"
                     /note="pGEX_3 primer"
     CDS             complement(8823..9011)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gaattcccga ttatgtcttt tgcgcactcg gcttaaacca gttttcgctg gtgcgaaaaa
       61 agagtgtctt gtgacaccta aattcaaaat ctatcggtca gatttatacc gatttgattt
      121 tatatattct tgaataacat acgccgagtt atcacataaa agcgggaacc aatcatcaaa
      181 tttaaacttc attgcataat ccattaaact cttaaattct acgattcctt gttcatcaat
      241 aaactcaatc atttctttaa ttaatttata tctatctgtt gttgttttct ttaataattc
      301 atcaacatct acaccgccat aaactatcat atcttctttt tgatatttaa atttattagg
      361 atcgtccatg tgaagcatat atctcacaag acctttcaca cttcctgcaa tctgcggaat
      421 agtcgcattc aattcttctg ttattatttt tatctgttca taagatttat taccctcata
      481 catcactaga atatgataat gctctttttt catcctacct tctgtatcag tatccctatc
      541 atgtaatgga gcactacaaa ttgaatgtgt aactctttta aatactctaa ccactcggct
      601 ttgctgattc tggatataaa acaaatgtcc aattacgtcc tcttgaattt ttcttgtttt
      661 cagtttcttt tattacattt tcgctcatga tataataacg gtgctaatac acttaacaaa
      721 atttagtcat agataggcag catgccagtg ctgtctatct ttttttgttt aaaatgcacc
      781 gtattcctcc tttgcatatt tttttattag aataccggtt gcatctgatt tgctaatatt
      841 atatttttct ttgattctat ttaatatctc attttcttct gttgtaagtc ttaaagtaac
      901 agcaactttt ttctcttctt ttctatctac aactatcact gtacctccca acatctgttt
      961 ttttcacttt aacataaaaa acaacctttt aacattaaaa acccaatatt tatttatttg
     1021 tttggacaat ggacaatgga cacctagggg ggaggtcgta gtacccccct atgttttctc
     1081 ccctaaataa ccccaaaaat ctaagaaaaa aagacctcaa aaaggtcttt aattaacatc
     1141 tcaaatttcg catttattcc aatttccttt ttgcgtgtga tgcgctgcgt ccattaaaaa
     1201 tcctagagct ttgcaaccga aagttaatag ctgtcgctac tactttcgct tacgctctaa
     1261 gtatatttta aggactgtca cacgcaaaaa gttttctcgg cataaaagta cctctacatc
     1321 tctaaatcgt ctgtacgctg tttctcacgc tttctatcga tcccgcaaga ggcccggcag
     1381 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     1441 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     1501 actaccgcat taaagcttgt cgacgattca caaaaaatag gcacacgaaa aacaagttaa
     1561 gggatgcagt ttatgcatcc cttaacttac ttattaaata atttatagct attgacaaga
     1621 gataagaatt gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa
     1681 ttgttaaatt gattttttgt aaatattttc ttgtattctt tgttaaccca tttcagaacg
     1741 aaataattat acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg
     1801 ataagtgagc tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat
     1861 atagaaatat caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat
     1921 cttttagcaa acccgtattc cacgattaaa taaatctcat tagctatact atcaagaaca
     1981 attttgcgta ttatatccgt acttatgtta taaggtatat taccatatat tttataggat
     2041 tggtttttag gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga
     2101 tcaacaagtt tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg
     2161 aaattacacc tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag
     2221 atattatcat gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa
     2281 gtaataaagt tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg
     2341 gttatatgaa ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat
     2401 aatgaactgt gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa
     2461 ttagtataat tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt
     2521 atactgcaat cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt
     2581 ttcttgtaaa aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt
     2641 taacgactta attacgaagt aaataagtct agtgtgttag actttatgaa atctttatac
     2701 gtttatatat atttattatc cggaggtgta gcatgtctca ttcaattttg agggttgcca
     2761 gagttaaagg atcaagtaat acaaacggga tacaaagaca taatcaaaga gagaataaaa
     2821 actataataa taaagacata aatcatgagg aaacatataa aaattatgat ttgattaacg
     2881 cacaaaatat aaagtataaa gataaaattg atgaaacgat tgatgagaat tattcaggga
     2941 aacgtaaaat tcggtcagat gcaattcgcc ccgggactag tttcgttatg gcttaaattg
     3001 caataacgct ttttgggatg gtcaggaaat tctttatgga gatggagata agaagaatta
     3061 caaaccattt tcatgtgcaa aaaatatcgt cgctcatgaa ttaacgcatg ctgtcattca
     3121 acactcagca ggtttagaat acgaagggca agcaggtgca ttaaatgaat cattcgcgga
     3181 tgtctttagt tattttatta ctccagacaa ttggttaata ggtgaggatg tctgtttaca
     3241 tggaatgaac agcaggagag taagaagttt aaaagaacca gataaataca accaagcggc
     3301 acacatgaat gagtatgagt caatgccgat tacagaagaa tatgattggg gaggggttca
     3361 tttcaatagc ggaataccta acaaagctgc ttataatacc atcacgaaag ttggtaggga
     3421 acaggcagaa caactatatt ttcgagcatt aaagtattac ttaacgaaaa aatcccaatt
     3481 tgtcgatgct aagaatgcgc ttcaacaggc ggcgagagac ttatacagcg aagaagtggc
     3541 taaaaaagta ggagaagcat gggaagaagt tggcgttaga taagggatgt cctccaagcc
     3601 aaattaacaa atgttaaaga aaagcggttt ctcccaatgc tattcttgaa gcaattcatg
     3661 aattgttttc ctaaattagc taatagggag gttttaacaa gagtcaaaaa ctctaaacag
     3721 cctgaattgt atactgttct tacgcaggta ttagagagcg gccgccagtg tgatggatat
     3781 ctgcagaatt aattcggctt tctagagtga cttttatgtt gaggcattaa catttgttaa
     3841 cgacgataaa gggacagcag gactagaata aagctataaa gcaagcatat aatattgcgt
     3901 ttcatcttta gaagcgaatt tcgccaatat tataattatc aaaagagagg ggtggcaaac
     3961 ggtatttggc attattaggt taaaaaatgt agaaggagag tgaaacccat gaaaaaaata
     4021 atgctagttt ttattacact tatattagtt agtctaccaa ttgcgcaaca aactgaagca
     4081 aaggatgcat cggatcttaa agctgtttat aattttgcta ctatgaagga tccatatcca
     4141 tatgatgttc cagattatgc atcattgaat tcaatgagaa gtgaaagacc acaagctttg
     4201 catcaaaaac gtacagcaat gttccaagat ccacaagaat ctggtcgtaa attaccacaa
     4261 ttatgtacag aattacaaac aacaattcat gatattattt tagaatgtgt ttattgtaaa
     4321 caacaattat tacgtcgtga agtttatgat ttcgcattcc gtgatttatg tattgtttat
     4381 cgtgatggta acccatatgc agtttgtgat aaatgtttaa aattctattc taaaatttct
     4441 gaatatcgtc atcatggtga tacaccaaca ttacatgaat atatgttaga tttacaacca
     4501 gaaacaacag atttatatgg ttatggtcaa ttaaacgatt cttctgaaga agaagatgaa
     4561 attgatggtc cagcaggtca agcagaacca gatcgtgcac attataacat tgttacattc
     4621 tgttgtaaat gtgattctac attagataaa tgtttaaaat tctattctaa aatttctgaa
     4681 tatcgtcatt attgttattc tgtttatggt acaacattag aacaacaata taacaaacca
     4741 ttatgtgatt tattaattcg ttgtattaac tgtcaaaaac cattatgtcc agaagaaaaa
     4801 caacgtcatt tagataaaaa acaacgtttc cataacattc gtggtcgttg gacaggtcgt
     4861 tgtatgtctt gttgtcgttc ttctcgtaca cgtcgtgaaa cacaattaca ttataacatt
     4921 gttacattct gttgtaaatg tgattctaca ttacgtttat gtgttcaatc tacacatgtt
     4981 gatattcgta cattagaaga tttattaatg ggtacattag gtattgtttg tccaatttgt
     5041 tctcaaaaac cactcgaggg attaaatgga ccagatattt ataaaggagt ttatcaattt
     5101 aaaagtgttg aatttgatgt cgagtataca gatattgaaa tgaatagatt aggaaaatga
     5161 taagtcggcg gccgcacaaa aaacggaaat cagttagtaa aactggtttc cgttttttat
     5221 taatagtctt gagcctcaat aacaacagca gtgccagatg ctgtaaccat taacatacca
     5281 ttatccgctc caagtacttc ataatcgata tccacaccaa ttacggcatt tgctccgata
     5341 tctttggcgc gctgttccat ctctttaata gcttcctcac gtgcgttaat taactcgtct
     5401 tcataacctt gtgatcgtcc accgaaaaag tttctgaggc cagctccaat gtctttcata
     5461 aaattaacac cagtaattac ttctccgaaa acgatttttt tatattcgat aatttgcttg
     5521 ccttcaatat ttggtgaggt agttacaatc attattaaat ccctccagtt ttcttttatc
     5581 atacctctaa gttgtttttt tagtcaaagg atagctggtt attttttacg ggcgtgggct
     5641 ttttcgatat aagtaatcgc atctgggact ttgcaagcag tttttcctaa atctacatat
     5701 actttaccta ttgattctgc tgcttccaat gcttttgtgt aaagttcctc tttataaatt
     5761 ccgcaagcga tgatataact attcatagag taacgagttc tgttcgtttc agattgtaaa
     5821 gattcttttg cgagttccag taattcctcg gcttcaggca aagtcgcgat gtgttctgtg
     5881 aaaatttgcc agcccattga tttagttaaa tcagcatcac ttttaatcca tttcctagca
     5941 aaggtgagtg cgtcatcacg tttacttaca acggaggcta gcccaaaagt taacatcgtc
     6001 caagcatctc gaaaagctat attccatttc tcgatttgtg aaacagctac ttttttaggg
     6061 ttcacggcaa gtaaaccaag gtaaattaag tcgctattat tagactcgat taattggagt
     6121 gctaattcgt gatttttatt caatttttca cggcgaataa ttttctttaa atcaccaatc
     6181 tttaatctct agaacttttt taagtgtatc tgcggagttt ttcatttgtt ctttttcttt
     6241 gtcatttaag ttcatttcaa caatatggcg aacgccttga cggttaacga ctgctggtgc
     6301 acctatataa atatcgttca taccgtaatg gccatctaaa taaacagaaa gtggcaaaat
     6361 cgcattttcg ttatttagaa ttgcttttgt aatacgagca agagctgcag caacgccgta
     6421 gaatgtagcg ccttttttat taataatttc ataagctgca tcacgaacac ttacgaaaat
     6481 agtatccatt gcaccttgtt catcttcgct aatccattca gtaattggaa ggccgccgac
     6541 agttgtgtgg ctccatgctg ggaattcttg aagacgaaag ggcctcgtga tacgcctatt
     6601 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6661 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6721 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6781 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6841 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6901 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6961 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     7021 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7081 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7141 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7201 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7261 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     7321 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7381 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7441 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7501 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7561 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7621 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7681 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7741 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7801 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7861 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7921 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7981 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8041 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8101 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8161 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8221 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8281 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8341 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8401 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8461 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8521 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8581 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     8641 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     8701 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     8761 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8821 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     8881 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     8941 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     9001 tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac
     9061 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     9121 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     9181 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     9241 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     9301 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
     9361 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     9421 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     9481 gccaggaccc aacgctgccc gacgatgata agctgtcaaa catga
//
