Basic Vector Information
- Vector Name:
- pLX-TuRV
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8306 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Host:
- Plants
- Source/Author:
- Pasin F.
- Promoter:
- CaMV 35S (enhanced)
pLX-TuRV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-TuRV vector Sequence
LOCUS 62056_16350 8306 bp DNA circular SYN 17-FEB-2021 DEFINITION Cloning vector pLX-TuRV, complete sequence. ACCESSION MW281335 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8306) AUTHORS Pasin F. TITLE Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity JOURNAL Biotechnol J, e2000354 (2021) In press PUBMED 33410597 REFERENCE 2 (bases 1 to 8306) AUTHORS Pasin F. TITLE Direct Submission JOURNAL Submitted (19-NOV-2020) Agricultural Biotechnology Research Center, Academia Sinica, n.a., Taipei, Taipei 11529, Taiwan REFERENCE 3 (bases 1 to 8306) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol J, e2000354 (2021) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-NOV-2020) Agricultural Biotechnology Research Center, Academia Sinica, n.a., Taipei, Taipei 11529, Taiwan" FEATURES Location/Qualifiers source 1..8306 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 25..72 /label=bacterial terminator /note="bacterial terminator" /regulatory_class="terminator" misc_feature 133..157 /label=LB T-DNA repeat /note="left border repeat from octopine Ach5 T-DNA" misc_feature 184..208 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" promoter 535..1203 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 1200..2777 /note="Synthetic fragment; BB.TuRV_1" misc_feature 1204..5289 /note="derived from turnip rosette virus INSD accession KC778720.1" misc_feature 2774..4157 /note="Synthetic fragment; BB.TuRV_2" misc_feature 2774..2777 /label=TTGT (AarI) /note="TTGT (AarI)" misc_feature 4154..5347 /note="Synthetic fragment; BB.TuRV_3" misc_feature 4154..4157 /label=ACAC (AarI) /note="ACAC (AarI)" misc_feature 5289..5334 /label=ribozyme /note="ribozyme" misc_feature 5344..5347 /label=CCCA (AarI) /note="CCCA (AarI)" terminator 5355..5607 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 5672..5695 /label=RB_pTiA6 /note="RB_pTiA6" misc_feature 5705..5733 /label=RB_overdrive_pTiA6 /note="RB_overdrive_pTiA6" regulatory complement(5760..5800) /label=bacterial terminator /note="bacterial terminator" /regulatory_class="terminator" CDS complement(5847..6659) /label=KanR /note="aminoglycoside phosphotransferase" CDS complement(6788..7447) /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" rep_origin complement(7448..8219) /direction=LEFT /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
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