LOCUS       62056_16350        8306 bp DNA     circular SYN 17-FEB-2021
DEFINITION  Cloning vector pLX-TuRV, complete sequence.
ACCESSION   MW281335
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Pasin F.
  TITLE     Oligonucleotide abundance biases aid design of a type IIS synthetic 
            genomics framework with plant virome capacity
  JOURNAL   Biotechnol J, e2000354 (2021) In press
  PUBMED    33410597
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Agricultural Biotechnology Research Center, 
            Academia Sinica, n.a., Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 8306)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J, e2000354 (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-NOV-2020) Agricultural Biotechnology Research Center, Academia 
            Sinica, n.a., Taipei, Taipei 11529, Taiwan"
FEATURES             Location/Qualifiers
     source          1..8306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1203
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1200..2777
                     /note="Synthetic fragment; BB.TuRV_1"
     misc_feature    1204..5289
                     /note="derived from turnip rosette virus INSD accession 
                     KC778720.1"
     misc_feature    2774..4157
                     /note="Synthetic fragment; BB.TuRV_2"
     misc_feature    2774..2777
                     /label=TTGT (AarI)
                     /note="TTGT (AarI)"
     misc_feature    4154..5347
                     /note="Synthetic fragment; BB.TuRV_3"
     misc_feature    4154..4157
                     /label=ACAC (AarI)
                     /note="ACAC (AarI)"
     misc_feature    5289..5334
                     /label=ribozyme
                     /note="ribozyme"
     misc_feature    5344..5347
                     /label=CCCA (AarI)
                     /note="CCCA (AarI)"
     terminator      5355..5607
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5672..5695
                     /label=RB_pTiA6
                     /note="RB_pTiA6"
     misc_feature    5705..5733
                     /label=RB_overdrive_pTiA6
                     /note="RB_overdrive_pTiA6"
     regulatory      complement(5760..5800)
                     /label=bacterial terminator
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(5847..6659)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(6788..7447)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(7448..8219)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcac cagtacgcac gattcaaggc ttgcttcaca aaccaaggca
      301 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
      361 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
      421 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
      481 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      541 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      601 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      661 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      721 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      781 atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
      841 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      901 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      961 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatc ctctgccgac
     1021 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     1081 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     1141 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     1201 aggcaaaata aatacaagaa agaaagattt tctcccacag cttgtattat ctctacgaca
     1261 ttaatgatta atgagtagag ttgccacaat cgaaatatac aacgagaacg gaataatcgt
     1321 agctcggaag aagacgtcgg gaccgcacgc gctcctagaa ctcttcaacg gaaagcagaa
     1381 atacgatcag gtgtccgaac tctttgtaat ttgggtttgt gaagagtgtg ggaaaaccgt
     1441 gtactctacg tgcgaattta aaggaatcgt atttgttaga gaggacggga aagagacaac
     1501 tgaattcgaa acagaagcag ttgtagactc cgacgattgt gggtgtgctt acgagtatca
     1561 ttccgagacc gagagtgaag cttgcctttg ccccggatac gcgatagaag gaatctgcga
     1621 ttgcgattgg tacgaagaca gacccgaaac cagcgacagc tctgagcttt tcacccagtg
     1681 ggaaaggctc gaactctttt ctgactaaaa tccacaagac atttactttc ttagtaatag
     1741 gtaagtttaa actgtggaat catgttgtca ttaaggagta tagtgaagct gatcgtagct
     1801 gcgttgaacg taatgtttgt cgtgacgata ggagtgtgtg ctcgagtgtt agccccagag
     1861 aggccagtga actggaactt tgtggcgcta ctgctgaccc cagtgctagc attgatagca
     1921 ttcgagctgc ttaccgagct aagaaaatgg atggtgtaca ccgtaaagga agaagacctt
     1981 ccagcacctc ttagcttgga ttcaactccg aggttcgacc ccattcacgg aataacgtca
     2041 acagtcaccg tggacggcaa ggtttaccag gtcgtaatac aacctgaata ctggcaccta
     2101 gtttccccga atcgatctca ggatggaaac aaggaaaccg tatgtataga ccgcatgtcc
     2161 actgtcaccc cggcaggaaa ggaaccacca tctctcgtga ccctcaaagt gggagataga
     2221 gttgtgggta tgggttcccg agtctcatgg ggaggcaata cgtacctgtt gactgcagcc
     2281 cacgtttgtg ccctacacaa ggacatctac atctataaga atgccatagg cactccacta
     2341 ggtgctggat ggactaggcg gtatggagct actcacaaga cagcagactt tactcttatc
     2401 gaagtcccac cgacggtgtg ggctaagctt ggagttaagg ctgcgagtct acaaccgttg
     2461 aacaaactta gcgtagtcac tgtttattcg gcaaatagct ccacggtaat aacttctagc
     2521 agctctcggg ctgttacaca agaattccgc catgtgataa tccactcgtg caatacaacg
     2581 gccggaacca gcggttctcc actttacagt ggggataatg tggtaggagt ccatttggga
     2641 actgaggtta ccatgcactc caacagagct tgtaatgtcg ggttggtctt aggggctttc
     2701 cacgaatcta taatctccca aggaactctt agtgagatct cagctgatga ggctgcggat
     2761 agagattatg attttgtgga tttcagcgtg gaaggattag ggaggttgtc aatgggaaaa
     2821 ggcgaatttt atttgcgaaa tgaccgagga attacaattg aagagatccg gaagaaagga
     2881 aggaaaatat ggacagaaga ctttgatgag gagtctgatg acggaatttt cgaaactcta
     2941 ccagtgagct ctttaaactg ccagcgggcg agcgacgaga ttgtctgctc gccctcctcg
     3001 agtttggaaa ttaccaatgg aaacaaggcc acgtcacaga agacggaatc ggcctcaaag
     3061 aagttgggaa gtccggggtt actttctcgg aaccaaagaa gaaaggaacg aaagcgatta
     3121 cagaagcttt ctcaagagaa atccccgaag tcgcaaacta cgcctggccc caaagaggat
     3181 caaaggccga aaggaagtcc ctctacctcc aagcgagtag gttccgaaga accgaagagc
     3241 caaatggaat cggagaggtt gttgagagac ttgtcaaaga gtatccaaca actaactgtc
     3301 ctcctgaatt caagggaagg tgggattacc aagacatctt cgagtacgtc acagacgtgg
     3361 cgcgctcgtc agacataaac ggcaaggcgt cccccggtgt gccgctctcc tcaatagcgg
     3421 ccaagaatga ggtgttagtg accagacatc tcgattttct tgttcatgct gtggtccaaa
     3481 gattgttcca tctttccgat gagattctcc cagaaaagcc ctcgccagag tggttggtgt
     3541 cccaaggtta ttgtgatcca gtgagagtct ttgttaaaca agaaccccac ccgttgagga
     3601 agcttgatga aggcagagtg agattaatca gctcggtgtc tctagtagac cagttagttg
     3661 aaagggtctt gttcggccgc caaaaccgga aagaaatcac acaatggaag agtatcccgt
     3721 cgaaacccgg aatgggcctg tcgctgactg aacaaatgaa gtcagtgttt gaacaggtga
     3781 gtaaattagc agctagccgt gaggcagctg aagctgatat ttccggtttc gattggtctg
     3841 tgcaagagtg ggaattggaa atggacttgg aggttcgcct ccggttagga aactttcccc
     3901 ctaagttgga gttagccgct cgaaatagat ttaaatgttt catgaactcg gttttccagc
     3961 ttagtaacgg tgaactgatc tctcaggtta gtcctggcct gatgaaatct ggatcctatt
     4021 gcacatcttc taccaactca agaatcaggg tagcaatggc gtatttaata ggctccccgt
     4081 ggtgtattgc catgggtgat gactctgtcg aaggttacgt taccaacgcc agagagaagt
     4141 atgaatccct cggacacatc tgtaaggatt acctggtttg ccaaaagaag aaaggcgagc
     4201 tagatgggtt caacttctgc tcccattgga tatcccgttc acattcttac cttgactcag
     4261 taggcaagac actctacaga tttctggaat catctaatga agaactagag atacttgagg
     4321 ctgagttagg gacccacccc agatggaggg aaattgtttc caaactagag ctcgttggaa
     4381 gaatccgagc aaaacataat ggagaaagga aacaagaagc tcacaaagaa ccagaggcgg
     4441 aaaacccgca agaagaattc ccaatctggg aggaccaatt cagtggttac acccttatgg
     4501 gccccagttt cgactgggac cgtaatgcag ggtggagcac tgtcaattag ttcgctgaat
     4561 aacaaaggtg acatacgagt gtgtggaagg gaagtagtca ccgaaatctc agtttcgaac
     4621 acgccaactc cttccgtgtt gcaagtcata ccagaaacct ttccttctag gttaaagggt
     4681 ctggctacca gttggtcgaa gttcaagtgg caggctatta aattcgttta catgcctatc
     4741 tgccccacaa ctgagagagg atctgttcac ttcggatttc tttacgacac cgtggataac
     4801 ctcccgggaa ctgtcggcga gatatccact ttacaaggtt acactactgg atctgtctgg
     4861 gccggaacct cgggaaacga gctactcgaa gatggtgttt acgccaaaac cccgaaggat
     4921 gccgtcgtag ccagaatgga cgctaggaga gctgataaga aatattatcc gatcgtgagt
     4981 accactcaat tgactaagtc tctaaacgtt gacgcttcgc tgggaaacac gtacgtgccg
     5041 gctcgcctgg cgatactgac cgctgatgga acaacggcag cagacaagcc ccaaatcgta
     5101 gggcgtctct acgccgtgtt ctgcgttgac ctgattgaca cgatcgcctc gtccttaaac
     5161 gtatagaatg gcccacttta cgggcactct tccctcacta cccaggtgag ggatgtagaa
     5221 ttcatttggg aaccccacat ggtagttagg actactccgc gtcaaagaca ggcggttatc
     5281 tgtctttggt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaaccaaaga
     5341 cagcccagcc tgcggatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     5401 ttgccggtct tgcgatgatt atcatctaat ttctgttgaa ttacgttaag catgtaataa
     5461 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     5521 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     5581 gcgcggtgtc atctatgtta ctcgatctcg tcaggagacg ggacaaggat gcgagctagc
     5641 ctgcaggaat tgttgatttt gtgatgactg atggcaggat atatgcggtt gtaattcatt
     5701 tttattgtct aaatttctgt atttgtttgt ttgttcggtt gtaaattttt ttggaagaac
     5761 aagaaaagaa aaaacacccg ttagggtgtt tttagttagt gtggcgcgcc gacttgcgac
     5821 atgcggtcct ttgcaatcaa ctattagaaa aattcatcca gcatcagatg aaattgcagt
     5881 ttgttcatat ccggattatc aatgccatat ttctgaaaca gacgtttttg caggctcggg
     5941 ctaaattcgc ccaggcagtt ccacagaatg gccagatcct gataacgatc cgcaatgccc
     6001 acacggccca catcaatgca gccaatcagt ttgccttcat cgaaaatcag gttatccagg
     6061 ctaaaatcgc cgtgggtcac cacgctatcc gggctaaacg gcagcagttt atgcatttct
     6121 ttccacacct gttccaccgg ccagccgtta cgttcatcat caaaatcgct cgcatccacc
     6181 aggccgttgt tcatacggct ctgcgcctgg gccagacgaa acacacgatc gctgttaaac
     6241 gggcagttgc acaccggaat gctatgcaga cgacgcagaa acacggccag cgcatccaca
     6301 atgttttcgc cgctatccgg atattcttcc agcacctgaa acgcggtttt gcccggaatc
     6361 gcggtggtca gcagccacgc atcatccggg gtgcgaataa aatgtttaat ggtcggcagc
     6421 ggcataaatt cggtcagcca gttcagacgc accatttcat cggtcacatc gttcgccacg
     6481 ctgcctttgc catgtttcag aaacagttcc ggcgcatccg gtttgccata cagacgataa
     6541 atggtcgcgc cgctctgacc cacgttatca cgcgcccatt tatagccata cagatccgca
     6601 tccatgttgc tgttcagacg cggacggcta cagctcgttt cacgctgaat atggctcata
     6661 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     6721 ttatcttgtg caatgtaaca tcagagattt tgagacacaa gatcggattg gcggttatgc
     6781 ggttctaccg gcgcggcagc gttacccgtg tcggcggctc caacggctcg ccatcgtcca
     6841 gaaaacacgg ctcatcgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg
     6901 tttcggtcaa ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct
     6961 cctcgccggg gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca
     7021 ggtcgccatg ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac
     7081 tgaacaccga caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca
     7141 cgtaggccga cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca
     7201 ccaagtcctt gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg
     7261 tcttctggct gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca
     7321 gcattgccgc cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt
     7381 ccgtttgcac ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg
     7441 tggccatgct tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca
     7501 gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta
     7561 cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg
     7621 tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt
     7681 ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc
     7741 cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg
     7801 cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca
     7861 ggcgggcggg cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg
     7921 cgtcaaggcc aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg
     7981 tccaaccggc aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta
     8041 aaaaaattga tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga
     8101 aggctgggta gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc
     8161 atcagcttgt ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgct
     8221 cgcggccatc gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
     8281 agcccggaga gcaagcccgt aggggg
//
