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Gwiz-GFP-bmp2-m vector (V015174)

Basic Vector Information

Vector Name:
Gwiz-GFP-bmp2-m
Antibiotic Resistance:
Kanamycin
Length:
7080 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

Gwiz-GFP-bmp2-m vector Vector Map

Gwiz-GFP-bmp2-m7080 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900oriKanRCMV enhancerCMV promoterCMV intron AGFPBmp2bGH poly(A) signal

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Gwiz-GFP-bmp2-m vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1106        7080 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1228..2040)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     enhancer        2666..3045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3046..3249
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3385..4210
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             4246..4953
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     CDS             4978..6159
                     /gene="Bmp2"
                     /label=Bmp2
                     /note="Bone morphogenetic protein 2 from Mus musculus. 
                     Accession#: P21274"
     polyA_signal    6235..6459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"

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