LOCUS       V015174                 7080 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015174
VERSION     V015174
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1228..2040)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     enhancer        2666..3045
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3046..3249
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3385..4210
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             4246..4953
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     CDS             4978..6159
                     /gene="Bmp2"
                     /label="Bone morphogenetic protein 2"
                     /note="Bone morphogenetic protein 2 from Mus musculus.
                     Accession#: P21274"
     polyA_signal    6235..6459
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gggttataca gcatcaggtg atacgcagga agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactcgggg ggggggggcg ctgaggtctg cctcgtgaag
      961 aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg
     1021 agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct
     1081 ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa
     1141 aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg
     1201 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     1261 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     1321 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     1381 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     1441 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     1501 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     1561 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     1621 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     1681 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     1741 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     1801 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     1861 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     1921 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     1981 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat
     2041 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     2101 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt cccccccccc
     2161 ccattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2221 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2281 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2341 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2461 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2521 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2581 attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     2641 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     2701 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2761 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2821 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2881 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2941 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3001 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3061 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3121 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3181 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3241 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     3301 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
     3361 cggattcccc gtgccaagag tgacgtaagt accgcctata gactctatag gcacacccct
     3421 ttggctctta tgcatgctat actgtttttg gcttggggcc tatacacccc cgcttcctta
     3481 tgctataggt gatggtatag cttagcctat aggtgtgggt tattgaccat tattgaccac
     3541 tcccctattg gtgacgatac tttccattac taatccataa catggctctt tgccacaact
     3601 atctctattg gctatatgcc aatactctgt ccttcagaga ctgacacgga ctctgtattt
     3661 ttacaggatg gggtcccatt tattatttac aaattcacat atacaacaac gccgtccccc
     3721 gtgcccgcag tttttattaa acatagcgtg ggatctccac gcgaatctcg ggtacgtgtt
     3781 ccggacatgg gctcttctcc ggtagcggcg gagcttccac atccgagccc tggtcccatg
     3841 cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag gccagactta
     3901 ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg gtagggtatg
     3961 tgtctgaaaa tgagcgtgga gattgggctc gcacggctga cgcagatgga agacttaagg
     4021 cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag tcagaggtaa
     4081 ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta ctcgttgctg
     4141 ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt tccatgggtc
     4201 ttttctgcag tcaccgtcgt cgacacgggc gcgcaagaaa tggctagcaa aggagaagaa
     4261 ctcttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa cggccacaag
     4321 ttctctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac cctgaagttc
     4381 atctgcacta ctggcaaact gcctgttcca tggccaacac tagtcactac tctgtgctat
     4441 ggtgttcaat gcttttcaag atacccggat catatgaaac ggcatgactt tttcaagagt
     4501 gccatgcccg aaggttatgt acaggaaagg accatcttct tcaaagatga cggcaactac
     4561 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     4621 ggtattgact tcaaggaaga tggcaacatt ctgggacaca aattggaata caactataac
     4681 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt gaacttcaag
     4741 acccgccaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     4801 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     4861 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     4921 gctgggatta cacatggcat ggatgaactg tacaactccg gactcagatc tcgagctatg
     4981 gtggccggga cccgctgtct tctagtgttg ctgcttcccc aggtcctcct gggcggcgcg
     5041 gccggcctca ttccagagct gggccgcaag aagttcgccg cggcatccag ccgacccttg
     5101 tcccggcctt cggaagacgt cctcagcgaa tttgagttga ggctgctcag catgtttggc
     5161 ctgaagcaga gacccacccc cagcaaggac gtcgtggtgc ccccctatat gctagatctg
     5221 taccgcaggc actcaggcca gccaggagcg cccgccccag accaccggct ggagagggca
     5281 gccagccgcg ccaacaccgt gcgcagcttc catcacgaag aagccgtgga ggaacttcca
     5341 gagatgagtg ggaaaacggc ccggcgcttc ttcttcaatt taagttctgt ccccagtgac
     5401 gagtttctca catctgcaga actccagatc ttccgggaac agatacagga agctttggga
     5461 aacagtagtt tccagcaccg aattaatatt tatgaaatta taaagcctgc agcagccaac
     5521 ttgaaatttc ctgtgaccag actattggac accaggttag tgaatcagaa cacaagtcag
     5581 tgggagagct tcgacgtcac cccagctgtg atgcggtgga ccacacaggg acacaccaac
     5641 catgggtttg tggtggaagt ggcccattta gaggagaacc caggtgtctc caagagacat
     5701 gtgaggatta gcaggtcttt gcaccaagat gaacacagct ggtcacagat aaggccattg
     5761 ctagtgactt ttggacatga tggaaaagga catccgctcc acaaacgaga aaagcgtcaa
     5821 gccaaacaca aacagcggaa gcgcctcaag tccagctgca agagacaccc tttgtatgtg
     5881 gacttcagtg atgtggggtg gaatgactgg atcgtggcac ctccgggcta tcatgccttt
     5941 tactgccatg gggagtgtcc ttttcccctt gctgaccacc tgaactccac taaccatgcc
     6001 atagtgcaga ctctggtgaa ctctgtgaat tccaaaatcc ctaaggcatg ctgtgtcccc
     6061 acagagctca gcgcaatctc catgttgtac ctagatgaaa atgaaaaggt tgtgctaaaa
     6121 aattatcagg acatggttgt ggagggctgc gggtgtcgtt agggatccag atcacttctg
     6181 gctaataaaa gatcagagct ctagagatct gtgtgttggt tttttgtgga tctgctgtgc
     6241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6421 acaatagcag gcatgctggg gatgcggtgg gctctatggg tacctctctc tctctctctc
     6481 tctctctctc tctctctctc tctctggtac ctctctctct ctctctctct ctctctctct
     6541 ctctctctgg tacccaggtg ctgaagaatt gacccggttc ctcctgggcc agaaagaagc
     6601 aggcacatcc ccttctctgt gacacaccct gtccacgccc ctggttctta gttccagccc
     6661 cactcatagg acactcatag ctcaggaggg ctccgccttc aatcccaccc gctaaagtac
     6721 ttggagcggt ctctccctcc ctcatcagcc caccaaacca aacctagcct ccaagagtgg
     6781 gaagaaatta aagcaagata ggctattaag tgcagaggga gagaaaatgc ctccaacatg
     6841 tgaggaagta atgagagaaa tcatagaatt tcttccgctt cctcgctcac tgactcgctg
     6901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7021 aggaaccgta aaaaggccgc gttgctggcg tttccatagc tccgccccct aaaggccccc
//
