pLV3-CMV-MCS-3×HA-Puro vector (V014872)

MSDS

Basic Vector Information

Vector Name:: pLV3-CMV-MCS-3×HA-Puro

Antibiotic Resistance:: Ampicillin

Length:: 6822 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells, Lentivirus

Selection Marker:: Puro

Promoter:: CMV

5' Primer:: CMV-F

Growth Temperature:: 37℃

pLV3-CMV-MCS-3×HA-Puro vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLV3-CMV-MCS-3×HA-Puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_15205        6822 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        156..535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        536..739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             815..2152
                     /gene="Runx1"
                     /label=Runx1
                     /note="Runt-related transcription factor 1 from Mus
                     musculus. Accession#: Q03347"
     CDS             2231..2923
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     misc_feature    2949..3537
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3569..4045
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4085..4225
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4300..4755
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5037..5141
                     /label=AmpR promoter
     CDS             5142..5999
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6173..6761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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