LOCUS       V014872                 6822 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014872
VERSION     V014872
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     enhancer        156..535
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        536..739
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             815..2152
                     /gene="Runx1"
                     /label="Runt-related transcription factor 1"
                     /note="Runt-related transcription factor 1 from Mus
                     musculus. Accession#: Q03347"
     CDS             2231..2923
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    2949..3537
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3569..4045
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4085..4225
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4300..4755
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5037..5141
                     /label="AmpR promoter"
     CDS             5142..5999
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6173..6761
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctg gtaccggatc cgccaccatg gcttcagaca gcatttttga
      781 gtcatttcct tcatatccac agtgcttcat gagagatgcc agcacgagcc gccgcttcac
      841 gccgccttcc accgcgctga gccccggcaa gatgagcgag gcgctgccgc tgggcgcccc
      901 ggatggcggc gccgccctgg ccagcaagct gaggagcggc gaccgcagca tggtggaggt
      961 actagctgac caccctggcg agctagtgcg caccgacagc cccaacttcc tctgctccgt
     1021 gctacccact cactggcgct gcaacaagac cctgcccatc gctttcaagg tggtggcact
     1081 gggggacgtc ccggatggca ctctggtcac cgtcatggca ggcaacgatg aaaactactc
     1141 ggcagaactg agaaatgcta ccgcggccat gaagaaccag gtagcgagat tcaacgacct
     1201 caggtttgtc gggcggagcg gtagaggcaa gagcttcact ctgaccatca ccgtctttac
     1261 aaatccgcca caagttgcca cctaccatag agccatcaaa atcacagtgg acggcccccg
     1321 agaaccccga agacatcggc agaaactaga tgatcagacc aagcccggga gtttgtcctt
     1381 ttccgagcgg ctcagtgaat tggagcagct gcggcgcacg gccatgaggg tcagcccgca
     1441 ccacccagcc cccacgccca accctcgggc ctccttgaac cactccactg cctttaaccc
     1501 tcagcctcaa agtcagatgc aggatgccag gcagatccag ccatccccac cgtggtccta
     1561 tgaccagtcc taccagtacc tgggatccat cacctcttcc tctgtccacc cagcgacacc
     1621 catttcaccc ggccgtgcca gcggcatgac cagcctctct gcagaacttt ccagtcgact
     1681 ctcaacggct ccggacctga ccgccttcgg cgacccacgc cagttcccta ctctgccgtc
     1741 catctccgac ccgcgcatgc actacccagg cgccttcacc tactcgccgc ccgtcacgtc
     1801 gggcatcggc atcggcatgt cagccatgag ctcggcctct cgctaccaca cctacctgcc
     1861 gccgccctac cccggctcat cacaggcgca ggccgggccc ttccagaccg gctcgccctc
     1921 ctaccatcta tactacggcg cctcggccgg ttcctaccag ttctccatgg tgggcggaga
     1981 gagatcgccc ccgcgcatcc tgccgccctg caccaacgca tccaccggcg ccgcgctgct
     2041 caaccccagc ctccccagcc agagcgacgt ggtggagacc gagggcagcc atagcaactc
     2101 gcccaccaac atgccccccg cgcgcctgga ggaggccgtg tggcggccct acagtctaga
     2161 gtcgacagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     2221 ttccggaacc atggcccagt ccaagcacgg cctgaccaag gagatgacca tgaagtaccg
     2281 catggagggc tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc
     2341 cttcaagggc aagcaggcca tcaacctgtg cgtggtggag ggcggcccct tgcccttcgc
     2401 cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc gtgttcaccg agtaccccca
     2461 ggacatcgtc gactacttca agaactcctg ccccgccggc tacacctggg accgctcctt
     2521 cctgttcgag gacggcgccg tgtgcatctg caacgccgac atcaccgtga gcgtggagga
     2581 gaactgcatg taccacgagt ccaagttcta cggcgtgaac ttccccgccg acggccccgt
     2641 gatgaagaag atgaccgaca actgggagcc ctcctgcgag aagatcatcc ccgtgcccaa
     2701 gcagggcatc ttgaagggcg acgtgagcat gtacctgctg ctgaaggacg gtggccgctt
     2761 gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg ccccgcaaga tgcccgactg
     2821 gcacttcatc cagcacaagc tgacccgcga ggaccgcagc gacgccaaga accagaagtg
     2881 gcacctgacc gagcacgcca tcgcctccgg ctccgccttg ccctgaattc gatatcaagc
     2941 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3001 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3061 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3121 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3181 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3241 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3301 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3361 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3421 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3481 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3541 gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc
     3601 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     3661 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     3721 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     3781 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     3841 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     3901 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     3961 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     4021 aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg
     4081 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     4141 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     4201 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     4261 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     4321 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4381 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4441 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4501 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4561 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4621 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     4681 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4741 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4801 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4861 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4921 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4981 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5161 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5221 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5281 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5341 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5401 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5461 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5521 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5581 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5641 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5701 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5761 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5821 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5881 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5941 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6001 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6061 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6301 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
