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pLac-maoA(opt-ecoli1917)-HA vector (V014825)

Basic Vector Information

Vector Name:
pLac-maoA(opt-ecoli1917)-HA
Antibiotic Resistance:
Kanamycin
Length:
4656 bp
Type:
Protein expression
Replication origin:
p15A ori
Host:
E. coli
Promoter:
Lac
Growth Temperature:
37℃

pLac-maoA(opt-ecoli1917)-HA vector Vector Map

pLac-maoA(opt-ecoli1917)-HA4656 bp600120018002400300036004200p15A oriCAP binding sitelac promotertynAHAKanR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLac-maoA(opt-ecoli1917)-HA vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_13680        4656 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(260..805)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    935..956
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        971..1001
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             1046..3316
                     /gene="tynA"
                     /label=tynA
                     /note="Primary amine oxidase from Escherichia coli (strain
                     K12). Accession#: P46883"
     CDS             3317..3343
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(3527..4333)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"

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