LOCUS       V014825                 4656 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014825
VERSION     V014825
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(260..805)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    935..956
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        971..1001
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             1046..3316
                     /gene="tynA"
                     /label="Primary amine oxidase"
                     /note="Primary amine oxidase from Escherichia coli (strain
                     K12). Accession#: P46883"
     CDS             3317..3343
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(3527..4333)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
       61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
      121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
      181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
      241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
      301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
      481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
      601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
      661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
      721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
      781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta
      841 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
      901 gttgtaattc tgccttttta cggttcctgg ccgctaatgt gagttagctc actcattagg
      961 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatg tctcatgagc
     1021 ggatactttc acacaggaaa cagctatggg tagcccgagt ctgtatagcg cgcgtaaaac
     1081 caccctggcg ctggcagttg cgctgagctt tgcatggcag gccccggtgt tcgctcatgg
     1141 tggtgaagca cacatggtgc cgatggataa aaccctgaaa gaatttggtg cagatgtgca
     1201 gtgggatgat tacgcgcagc tgtttaccct gattaaagat ggcgcgtacg tgaaagtgaa
     1261 accgggtgcc cagaccgcca ttgtgaatgg tcagccgctg gccctgcaag tgccggtggt
     1321 gatgaaagat aacaaagcct gggtgagcga taccttcatc aacgatgtgt tccagagcgg
     1381 tctggaccaa acctttcagg tggaaaagcg tccgcatccg ctgaatgctc tgacggcaga
     1441 tgaaattaaa caggccgtgg aaatcgtgaa agcaagcgca gatttcaaac cgaacacccg
     1501 tttcaccgaa atcagcctgc tgccgccgga taaagaagcc gtgtgggctt ttgccctgga
     1561 aaacaaacca gttgaccaac cgcgtaaagc cgatgtgatt atgctggatg gcaaacacat
     1621 catcgaagcc gtggtcgatt tacagaacaa caaactgctg agctggcagc cgattaaaga
     1681 tgcccacggt atggtcctgc tggatgattt tgcctcagtg cagaacatca tcaacaacag
     1741 cgaagaattt gcagcagcag tgaagaagcg cggtattaca gatgccaaga aggtgattac
     1801 caccccgctg accgtgggtt attttgatgg taaagatggt ctgaaacagg atgcacgcct
     1861 gctgaaagtg attagctacc tggatgtggg ggatggcaat tattgggccc atccgattga
     1921 aaacctggtg gccgtggtgg atttagaaca gaagaagatt gtgaaaatcg aagaaggtcc
     1981 ggtggtgccg gtgccgatga ccgcacgtcc gttcgatggt cgtgatagag tggcgccggc
     2041 cgttaaaccg atgcagatta ttgaaccgga aggtaaaaac tacaccatca ccggggatat
     2101 gattcactgg cgtaactggg atttccacct gagcatgaac agccgcgtgg gtccgatgat
     2161 tagcaccgtg acctacaacg ataacggcac aaaacgcaaa gtgatgtacg aagggagcct
     2221 gggtggtatg attgtgccgt acggcgaccc tgatattgga tggtatttca aagcatacct
     2281 ggatagcggt gattacggca tgggtacact gaccagcccg attgcccgtg gtaaagatgc
     2341 cccgagcaac gcagttctgc tgaatgaaac catcgccgat tacaccggcg tgccgatgga
     2401 aattccgcgt gctattgccg ttttcgaacg ctatgccggt ccagaataca aacaccagga
     2461 aatgggccag ccgaacgtta gcacagaacg ccgtgaactg gtggtgcgtt ggattagcac
     2521 ggttggtaat tacgattaca tcttcgattg gattttccac gaaaacggta cgattggtat
     2581 cgacgccggt gccaccggaa ttgaagctgt gaaaggtgtg aaagcaaaaa ccatgcacga
     2641 tgaaaccgca aaagatgata cccgctacgg caccctgatt gaccataata tcgtggggac
     2701 cacccaccag cacatttaca atttccgcct ggatttagat gtggatggtg aaaacaacag
     2761 cctggtcgca atggaccctg ttgtgaaacc gaataccgcc ggtggtccgc gtacatcaac
     2821 catgcaggtg aaccagtaca acatcggtaa cgaacaggat gccgcccaga aattcgaccc
     2881 tggtacaatt cgtctgctga gcaacccgaa caaagaaaac cgtatgggca acccggtgag
     2941 ctatcagatt atcccgtacg ccggtggcac ccacccggtg gcaaaaggtg cacagtttgc
     3001 accggatgaa tggatttacc accgcctgag cttcatggat aaacagctgt gggtgacccg
     3061 ttaccacccg ggtgaacgtt ttccagaagg taaatacccg aaccgcagca cccatgatac
     3121 aggactgggc cagtatagca aagataacga aagcctggat aacaccgatg ccgtggtttg
     3181 gatgaccacc ggaaccaccc acgttgcacg cgcagaagaa tggccgatta tgccgacaga
     3241 atgggtgcat accctgctga aaccgtggaa tttcttcgat gaaaccccga ccctgggtgc
     3301 cctgaagaag gataaatacc cgtacgatgt gccggattac gcgtaagaag atcctttgat
     3361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3481 aagcccaatc tgaataatgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     3541 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     3601 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     3661 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     3721 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     3781 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     3841 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     3901 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     3961 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     4021 atgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     4081 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     4141 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     4201 gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     4261 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt
     4321 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     4381 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga
     4441 gctgcatcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4501 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4561 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4621 gagtgcacca tatgcggtgt gaaataccgc acagat
//
