Basic Vector Information
- Vector Name:
- pLV2-EF1a-GUCY2C(Cynomolgus-opt)-3×FLAG-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12119 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- EF-1α
- Growth Temperature:
- 37℃
pLV2-EF1a-GUCY2C(Cynomolgus-opt)-3×FLAG-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV2-EF1a-GUCY2C(Cynomolgus-opt)-3×FLAG-Puro vector Sequence
LOCUS V014780 12119 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V014780
VERSION V014780
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 12119)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12119
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2027..2144
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2338..3519
/label="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 3548..6766
/gene="GUCY2C"
/label="Guanylyl cyclase C"
/note="Guanylyl cyclase C from Homo sapiens. Accession#:
P25092"
CDS 6776..6841
/label="3xFLAG"
/note="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
misc_feature 6869..7455
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 7475..8071
/label="PuroR"
/note="puromycin N-acetyltransferase"
misc_feature 8088..8676
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 8883..9516
/label="5' LTR"
/note="5' long terminal repeat (LTR) from HIV-1"
promoter complement(9692..9722)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(9737..9758)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(10046..10634)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10808..11665)
/label="AmpR"
/note="beta-lactamase"
promoter complement(11666..11770)
/label="AmpR promoter"
polyA_signal 11818..11952
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
This page is informational only.