LOCUS       V014780                12119 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014780
VERSION     V014780
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3548..6766
                     /gene="GUCY2C"
                     /label="Guanylyl cyclase C"
                     /note="Guanylyl cyclase C from Homo sapiens. Accession#:
                     P25092"
     CDS             6776..6841
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    6869..7455
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             7475..8071
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    8088..8676
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8883..9516
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9692..9722)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9737..9758)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10046..10634)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10808..11665)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11666..11770)
                     /label="AmpR promoter"
     polyA_signal    11818..11952
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg aagaccctgc tgctggacct ggttctgtgg tccctgctgt ttcagcccga
     3601 gtggctgtac ctgacaagcc aggtgtccca gaactgccac aacggcagct acgagatcag
     3661 cgtgctgatg atggacaaca gcgccttcgc cgagcctctg gaaaacgtgg aagatgccgt
     3721 gaacgaaggc ctggaaatcg tgcgaggcag actgcagaat gccggcctga acgtgacagt
     3781 gaacgccagc ttcatgtaca gcgacggcct gatccacaac agcggcgact gtagaagcag
     3841 cacctgtgaa ggactggacc tgctgcggaa gatcagcaac gccaagagaa tgggctgtgt
     3901 gctgatgggc cctagctgca cctacagcac cttccagatg tacctggaca ccgagctgag
     3961 ctaccccatg atcagcgccg gaagctttgg cctgagctgc gactacaaag agacactgac
     4021 ccggctgatg agccccgcca gaaagctgac ctacttcctg gtcaactttt ggaaaacgaa
     4081 cgacctgccg ttcaagacct acagctggtc caccagctac gtgtacaaga acggcaccga
     4141 gagcgaggac tgcttctggt atctgaacgc cctggaagcc tccgtgtcct actttagcca
     4201 cgagctgtcc ttcaagctgg tgctgcggca ggacaaagag ttccaggaca tcctgatgga
     4261 ccacaaccgg aagtccaacg tgatcgtgat gtgcggcgac cccgagttcc tgtacaagct
     4321 gaaaggcgat agagccgtgg ccgaggacat cgtgatcatc ctggtggatc tgttcaacga
     4381 ccagtacttc gaggacaatg tgacagcccc tgactacatg aagaacgtgc tggtcctgac
     4441 aagaagcccc ggcaacagcc tgctgaacag cagcttcagc agaaatctga gccccaccaa
     4501 gcgggatttc gccctggcct atctgaatgg catcctgctg ttcggccaca tgctgaaaac
     4561 attcctcgag aacggcgaga acatcacgac ccctaagttc gcccacgcct tccggaatct
     4621 gaccttcgag ggctatgacg gccccgtgac acttgatgac tggggcgacg tggacagcac
     4681 aatggtgctg ctgtacacca gcgtggacac caaaaagtac aaagtgctgc tgacatacga
     4741 cacccacgtg aaccagacaa accccgtgga catgagccct accttcacct ggaagaacag
     4801 caagctgccc aacgacatca ccgacagagg ccctcagatc ctgatgatcg ccgtgttcac
     4861 actgacaggc gccgtggttc tgctgctgct tgttgctctg ctgatgctga ggaagtacaa
     4921 gaaggactac gagctgaggc agaagaagtg gtcccacatt cctccagaga acatcttccc
     4981 actggaaaca aacgagacta accacgtgtc cctgaagatc gacgacgaca agcggagaga
     5041 caccatccag agactgcggc agtgcaaata cgacaagaaa cgcgtgatcc tgaaggacct
     5101 gaagcacaac gacggcaact tcaccgagaa gcagaagatc gagctgaaca agctgctgca
     5161 gatcgactac tacaacctga ccaagttcta cggcaccgtg aagctggaca ccatgatctt
     5221 cggcgtgatc gagtactgcg agcggggctc tctgagagag gtgctgaacg acaccatcag
     5281 ctaccccgat ggcaccttca tggactggga gttcaagatc tccgtgctgt acgatatcgc
     5341 caagggcatg agctacctgc acagcagcaa gacagaggtg cacggcagac tgaagtccac
     5401 caactgcgtg gtggatagcc ggatggtggt caagatcacc gacttcggct gcaacagcat
     5461 cctgcctcca aagaaagacc tgtggacagc cccagagcac ctgagacagg ccaatgtgtc
     5521 tcagaaaggc gacgtgtact cctacggcat cattgcccaa gagatcatcc tccggaaaga
     5581 gacattctac accagcagct gccgggacag aaacgagaag atctttcgcg tggaaaactc
     5641 caacggcatg aagcccttca gacccgacct gttcctggaa accgccgaag agaaagaact
     5701 ggaagtgtac ctgctggtca agagctgctg ggaagaagat cccgagaagc ggcccgactt
     5761 caagaagatt gagacaaccc tggccaagat cttcggactg ttccacgatc agaaaaacga
     5821 gagctacatg gataccctga tccggcggct gcagctgtac tccagaaacc tggaacacct
     5881 ggtggaagag agaacccagc tgtacaaggc cgagagagac agagccgacc ggctgaactt
     5941 tatgctgctg cccagactgg tcgtgaagtc cctgaaagag aagggctttg tcgagcccga
     6001 gctgtacgag gaagtgacca tctacttcag cgacatcgtg ggcttcacca ccatctgcaa
     6061 gtacagcacc cctatggaag tggtggacat gctcaacgat atctacaaga gcttcgacca
     6121 catcgtggac caccacgacg tctacaaggt ggaaacaatc ggcgacgcct acatggtggc
     6181 ctctggcctg cctaagagaa acggcaacag acacgccatc gacattgcca agatggccct
     6241 ggaaattctg agcttcatgg gcaccttcga gctggaacat ctgcccggac tgcccatctg
     6301 gatcagaatc ggagtgcaca gcggaccttg tgccgctggt gttgtgggca tcaagatgcc
     6361 tcggtactgc ctgttcggcg acaccgtgaa taccgccagc agaatggaaa gcaccggcct
     6421 gcctctgcgg atccatgtgt ctggaagcac aatcgccatc ctgaagcgga ccgagtgcca
     6481 gtttctgtac gaagtgcggg gcgagacata cctgaaaggc agaggcaacg agacaaccta
     6541 ctggctgaca ggcatgaagg atcagaagtt caacctgcct acacctccta ccgtggaaaa
     6601 tcagcagcga ctgcaggccg agttctccga catgatcgcc aatagcctgc agaagagaca
     6661 ggctgccggc atcagaagcc aaaagcctag aagagtggcc agctataaga agggcaccct
     6721 ggaatacctg cagctgaaca ccaccgacaa agagagcacc tacttccgcg gccgcgacta
     6781 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     6841 gtaggcctgc gatatccagc ggatcccgcc cctctccctc ccccccccct aacgttactg
     6901 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6961 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     7021 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     7081 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     7141 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     7201 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     7261 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     7321 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     7381 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     7441 aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg
     7501 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     7561 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     7621 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7681 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7741 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7801 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7861 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7921 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7981 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8041 cacctggtgc atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg
     8101 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8161 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8221 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8281 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8341 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8401 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8461 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     8521 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8581 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8641 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     8701 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8761 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8821 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     8881 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     8941 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9001 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9061 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9121 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9181 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9241 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9301 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9361 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9421 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9481 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9541 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct
     9601 agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    11821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    11881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11941 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    12001 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    12061 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
