Basic Vector Information
- Vector Name:
- pLV3-CAG-lacO-Fluc-GFP-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11227 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- CAG
- Growth Temperature:
- 37℃
pLV3-CAG-lacO-Fluc-GFP-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV3-CAG-lacO-Fluc-GFP-Puro vector Sequence
LOCUS V014764 11227 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V014764
VERSION V014764
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 11227)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..11227
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 6..233
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 234..414
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1804..1920
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
intron 2602..3619
/label="chimeric intron"
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
protein_bind 3674..3698
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 3718..5367
/label="luciferase"
/note="firefly luciferase"
CDS 5443..6159
/label="EGFP"
/note="enhanced GFP"
promoter 6203..6414
/label="EF-1-alpha core promoter"
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 6427..6695
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 6722..7318
/label="PuroR"
/note="puromycin N-acetyltransferase"
misc_feature 7322..7910
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 7984..8217
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 8289..8423
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 8429..8564
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(8650..8680)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(8695..8716)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9004..9592)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9766..10623)
/label="AmpR"
/note="beta-lactamase"
promoter complement(10624..10728)
/label="AmpR promoter"
primer_bind 11202..11218
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
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