LOCUS       V014764                11227 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014764
VERSION     V014764
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     intron          2602..3619
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     protein_bind    3674..3698
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3718..5367
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             5443..6159
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        6203..6414
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             6427..6695
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6722..7318
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7322..7910
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7984..8217
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8289..8423
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8429..8564
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8650..8680)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8695..8716)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9004..9592)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9766..10623)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10624..10728)
                     /label="AmpR promoter"
     primer_bind     11202..11218
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgataagc ttgggagttc cggacattga ttattgacta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     2341 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     2401 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg
     2461 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag
     2521 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     2581 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc
     2641 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     2701 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     2761 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     2821 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     2881 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     2941 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     3001 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     3061 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     3121 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     3181 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg
     3241 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     3301 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     3361 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     3421 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     3481 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     3541 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     3601 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     3661 tggcaaagaa ttcggaattg tgagcggata acaattcccc tctagaaata agccaccatg
     3721 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
     3781 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
     3841 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
     3901 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
     3961 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
     4021 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
     4081 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
     4141 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
     4201 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
     4261 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
     4321 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
     4381 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
     4441 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
     4501 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
     4561 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
     4621 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
     4681 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
     4741 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
     4801 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
     4861 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
     4921 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     4981 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     5041 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     5101 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     5161 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     5221 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     5281 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     5341 gccaagaagg gcggcaagat cgccgtgtta gcggccgctg agggcagagg aagtcttcta
     5401 acatgcggtg acgtggagga gaatcccggc ccttccggaa ccatggtgag caagggcgag
     5461 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5521 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5581 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5641 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5701 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5761 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5821 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5881 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5941 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6001 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6061 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6121 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc aaggatctgc
     6181 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     6241 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6301 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6361 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     6421 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6481 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     6541 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     6601 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6661 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6721 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6781 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6841 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6901 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6961 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7021 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7081 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7141 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7201 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7261 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7321 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7381 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7441 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7501 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7561 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7621 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7681 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7741 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7801 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7861 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt
     7921 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7981 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt
     8041 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8101 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8161 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8221 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8281 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8401 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca
     8461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga
     8581 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8821 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8881 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8941 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9421 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10741 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10801 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10861 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10921 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10981 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11041 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11101 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11161 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11221 caagctg
//
