Basic Vector Information
- Vector Name:
- pLenti-SFFV-ZsGreen-P2A-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10029 bp
- Type:
- Gene expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- SFFV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLenti-SFFV-ZsGreen-P2A-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti-SFFV-ZsGreen-P2A-Puro vector Sequence
LOCUS 62056_13885 10029 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10029)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10029
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..199
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 217..397
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 444..569
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1062..1295
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1480..1524
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1673..1714
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 1822..1939
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2671..3078
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 3092..3784
/label=ZsGreen1
/note="Zoanthus green fluorescent protein"
CDS 3801..3824
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 3834..3890
/label=P2A
/note="2A peptide from porcine teschovirus-1 polyprotein"
CDS 3891..4484
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 4503..5091
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5163..5396
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5428..5652
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5698..6126
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6140..6469
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 6517..6564
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 6583..6954
/label=BleoR
/note="antibiotic-binding protein"
polyA_signal 7087..7220
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(7305..7335)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7350..7371)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7659..8247)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8421..9278)
/label=AmpR
/note="beta-lactamase"
promoter complement(9279..9383)
/label=AmpR promoter
promoter 10000..10029
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
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