LOCUS       V014576                10029 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014576
VERSION     V014576
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             217..397
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1062..1295
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1480..1524
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1673..1714
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1822..1939
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2671..3078
                     /label="SFFV promoter"
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     CDS             3092..3784
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     CDS             3801..3824
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             3834..3890
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             3891..4484
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4503..5091
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5163..5396
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5428..5652
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5698..6126
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6140..6469
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6517..6564
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6583..6954
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7087..7220
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(7305..7335)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7350..7371)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7659..8247)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8421..9278)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9279..9383)
                     /label="AmpR promoter"
     promoter        10000..10029
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccc gggaggtggt ccctgcagtt acgccaatga taacccccgc cagaaaaatc
     2041 ttagtagcct tccctttttg ttttccgtgc cccaactcgg cggattgact cggccccttc
     2101 cggaaacacc cgaatcaact tctagtcaaa ttattgttca cgccgcaatg acccacccct
     2161 ggcccgcgtc tgtggaactg acccctggtg tacaggagag ttcgctgctg aaagtggtcc
     2221 caaaggggta ctagttttta agctcccaac tccccctccc ccagcgtctg gaggattcca
     2281 caccctcgca ccgcaggggc gaggaagtgg gcggagtccg gttttggcgc cagccgctga
     2341 ggctgccaag cagaaaagcc accgctgagg agactccggt cactgtcctc gccccgcctc
     2401 ccccttccct ccccttgggg accaccgggc gccacgccgc gaacgttaag tgccgcggtc
     2461 gtcggcgcct ccgccctccc ccttgggccc caattcccag cgggcgcggc gcgcggcccc
     2521 tccccccgcc gggcgcgcgc ccgctgcccc gcccttcgtg gccgcccggc gtgggcggtg
     2581 ccacccctcc ccccggcggc cccgcgcgca gctcccggct ccctccccct tcggatgtgg
     2641 cttgagctgt aggcgcggag gctagctgca gtaacgccat tttgcaaggc atggaaaaat
     2701 accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg
     2761 ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg
     2821 gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca
     2881 accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat
     2941 gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt
     3001 ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga
     3061 cagactgagt cgcccggggg aattcgccac catggcccag tccaagcacg gcctgaccaa
     3121 ggagatgacc atgaagtacc gcatggaggg ctgcgtggac ggccacaagt tcgtgatcac
     3181 cggcgagggc atcggctacc ccttcaaggg caagcaggcc atcaacctgt gcgtggtgga
     3241 gggcggcccc ttgcccttcg ccgaggacat cttgtccgcc gccttcatgt acggcaaccg
     3301 cgtgttcacc gagtaccccc aggacatcgt cgactacttc aagaactcct gccccgccgg
     3361 ctacacctgg gaccgctcct tcctgttcga ggacggcgcc gtgtgcatct gcaacgccga
     3421 catcaccgtg agcgtggagg agaactgcat gtaccacgag tccaagttct acggcgtgaa
     3481 cttccccgcc gacggccccg tgatgaagaa gatgaccgac aactgggagc cctcctgcga
     3541 gaagatcatc cccgtgccca agcagggcat cttgaagggc gacgtgagca tgtacctgct
     3601 gctgaaggac ggtggccgct tgcgctgcca gttcgacacc gtgtacaagg ccaagtccgt
     3661 gccccgcaag atgcccgact ggcacttcat ccagcacaag ctgacccgcg aggaccgcag
     3721 cgacgccaag aaccagaagt ggcacctgac cgagcacgcc atcgcctccg gctccgcctt
     3781 gccctgagcg gccgtctaga gattacaaag acgatgacga taagggatcc ggcgcaacaa
     3841 acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg accgagtaca
     3901 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3961 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     4021 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     4081 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     4141 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     4201 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4261 tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     4321 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     4381 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     4441 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttaagtcg
     4501 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4861 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     5101 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5161 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5221 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5281 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5341 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     5401 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5461 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5521 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5581 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5641 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5701 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5761 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5821 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5881 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5941 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6001 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6061 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6121 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6301 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6361 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6421 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6481 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     6541 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     6601 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     6661 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     6721 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     6781 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     6841 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     6901 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     6961 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7021 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7081 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7201 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9421 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     9481 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     9541 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     9601 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     9661 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     9721 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     9781 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     9841 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     9901 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     9961 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    10021 ttaccatgg
//
