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pMA5-IPK(opt-bs168) vector (V014368)

Basic Vector Information

Vector Name:
pMA5-IPK(opt-bs168)
Antibiotic Resistance:
Ampicillin
Length:
7924 bp
Type:
Protein expression
Replication origin:
ori
Host:
Bacillus subtilis
Selection Marker:
Kan
Promoter:
HpaII
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pMA5-IPK(opt-bs168) vector Vector Map

pMA5-IPK(opt-bs168)7924 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900720075007800Isopentenyl phosphate kinase from Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165). Accession#: Q9HLX16xHisfd terminatorf1 oriAmpR promoterAmpRoriCAP binding sitecat promoterrepB

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMA5-IPK(opt-bs168) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16445        7924 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..800
                     /note="Isopentenyl phosphate kinase from Thermoplasma 
                     acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
                     15155 / AMRC-C165). Accession#: Q9HLX1"
     CDS             801..818
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      865..913
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1151..1606
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1728..1832
                     /label=AmpR promoter
     CDS             1833..2690
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2864..3455
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3743..3764
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(4225..4327)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4866..5867
                     /label=repB
                     /note="RepB replication protein"

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