LOCUS       Exported                7921 bp DNA     circular SYN 01-NOV-2025
DEFINITION  Exported.
ACCESSION   V014368
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7921)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             66..800
                     /label=Isopentenyl phosphate kinase
                     /note="Isopentenyl phosphate kinase from Thermoplasma 
                     acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC
                     15155 / AMRC-C165). Accession#: Q9HLX1"
     CDS             801..818
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      865..913
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1151..1606
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1728..1832
                     /label=AmpR promoter
     CDS             1833..2690
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2864..3452
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3740..3761
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(4222..4324)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4863..5864
                     /label=repB
                     /note="RepB replication protein"
ORIGIN
        1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc atggatcctt
       61 tcacaatgat gatccttaaa atcggcggct ctgttatcac agataaatct gcttaccgta
      121 cagctcgtac atacgctatc cgttctatcg ttaaagttct ttctggcatc gaagatcttg
      181 tttgcgttgt tcatggcggc ggctctttcg gccatatcaa agctatggaa ttcggccttc
      241 ctggccctaa aaaccctcgt tcttctatcg gctactctat cgttcatcgt gatatggaaa
      301 accttgatct tatggttatc gatgctatga tcgaaatggg catgcgtcct atctctgttc
      361 ctatctctgc tcttcgttat gacggcaggt tcgattacac accacttatc cgttacatcg
      421 atgctggctt cgttcctgtt tcttacggcg atgtttacat caaagatgaa cattcttacg
      481 gcatctactc tggcgatgat atcatggctg atatggctga acttcttaaa cctgatgttg
      541 ctgttttcct tacagatgtt gatggcatct actctaaaga tcctaaacgt aaccctgatg
      601 ctgttcttct tcgtgatatc gatacaaaca tcacattcga tcgtgttcaa aacgatgtta
      661 caggcggcat cggcaaaaaa ttcgaatcta tggttaaaat gaaatcttct gttaagaatg
      721 gcgtgtacct gatcaacggc aaccatcctg aacgtatcgg cgatatcggc aaagaatctt
      781 tcatcggcac agttatccgt catcatcatc atcatcacta acgcgttcta gaggtcgaaa
      841 ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt
      901 ttttggagat tttcaacgtg aaaaaattat tattcgcaat tccaagctaa ttcacctcga
      961 aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt ttttggagat
     1021 tttcaacgtg aaaaaattat tattcgcaat tccaagctct gcctcgcgcg tttcggtgat
     1081 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1141 gatgcagatc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1201 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1261 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1321 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1381 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1441 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1501 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1561 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgatc tgcgctcggt
     1621 cgttcggctg cggcgagcgg tatcagctca ctcaaagcgg taatacggtt atcccagaat
     1681 ccggggataa cgcaggaaag acaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3781 actttatgct tccggcatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     3841 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     3901 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     3961 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     4021 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     4081 ggtctttaaa aaggccgtaa tatccagcta aacggtctgg ttataggtac attgagcaac
     4141 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     4201 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     4261 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     4321 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     4381 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcga agacgaaagg
     4441 gcatcgcgcg cggggaattc ccgggagagc tcgatatcgc atgcggtacc tctagaagaa
     4501 gcttggagac aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc
     4561 taaaaagaac gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga
     4621 tcagggaatg agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt
     4681 ttgaacttgt tctttcttat cttgatacat atagaaataa cgtcattttt attttagttg
     4741 ctgaaaggtg cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa
     4801 ttggttgcac agaaaaaccc catctgttaa agttataagt gactaaacaa ataactaaat
     4861 agatgggggt ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat
     4921 caagggtttt agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa
     4981 tcgctaatgt tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag
     5041 taaaagattg tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt
     5101 tgtatcgagt gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa
     5161 tgaaacatgg cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag
     5221 ttcgttggtt gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata
     5281 agagtttgtc agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata
     5341 aaaatcttgt tggttttatg cgtgcaacgg aagtgacaat aaataataaa gataattctt
     5401 ataatcagca catgcatgta ttggtatgtg tggaaccaac ttattttaag aatacagaaa
     5461 actacgtgaa tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa ttagactatg
     5521 atccaaatgt aaaagttcaa atgattcgac cgaaaaataa atataaatcg gatatacaat
     5581 cggcaattga cgaaactgca aaatatcctg taaaggatac ggattttatg accgatgatg
     5641 aagaaaagaa tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa
     5701 tctcctatgg tggtttgtta aaagaaatac ataaaaaatt aaaccttgat gacacagaag
     5761 aaggcgattt gattcataca gatgatgacg aaaaagccga tgaagatgga ttttctatta
     5821 ttgcaatgtg gaattgggaa cggaaaaatt attttattaa agagtagttc aacaaacggg
     5881 ccagtttgtt gaagattaga tgctataatt gttattaaaa ggattgaagg atgcttagga
     5941 agacgagtta ttaatagctg aataagaacg gtgctctcca aatattctta tttagaaaag
     6001 caaatctaaa attatctgaa aagggaatga gaatagtgaa tggaccaata ataatgacta
     6061 gagaagaaag aatgaagatt gttcatgaaa ttaaggaacg aatattggat aaatatgggg
     6121 atgatgttaa ggctattggt gtttatggct ctcttggtcg tcagactgat gggccctatt
     6181 cggatattga gatgatgtgt gtcatgtcaa cagaggaagc agagttcagc catgaatgga
     6241 caaccggtga gtggaaggtg gaagtgaatt ttgatagcga agagattcta ctagattatg
     6301 catctcaggt ggaatcagat tggccgctta cacatggtca atttttctct attttgccga
     6361 tttatgattc aggtggatac ttagagaaag tgtatcaaac tgctaaatcg gtagaagccc
     6421 aaacgttcca cgatgcgatt tgtgccctta tcgtagaaga gctgtttgaa tatgcaggca
     6481 aatggcgtaa tattcgtgtg caaggaccga caacatttct accatccttg actgtacagg
     6541 tagcaatggc aggtgccatg ttgattggtc tgcatcatcg catctgttat acgacgagcg
     6601 cttcggtctt aactgaagca gttaagcaat cagatcttcc ttcaggttat gaccatctgt
     6661 gccagttcgt aatgtctggt caactttccg actctgagaa acttctggaa tcgctagaga
     6721 atttctggaa tgggattcag gagtggacag aacgacacgg atatatagtg gatgtgtcaa
     6781 aacgcatacc attttgaacg atgacctcta ataattgtta atcatgttgg ttacgtattt
     6841 attaacttct cctagtatta gtaattatca tggctgtcat ggcgcattaa cggaataaag
     6901 ggtgtgctta aatcgggcca ttttgcgtaa taagaaaaag gattaattat gagcgaattg
     6961 aattaataat aaggtaatag atttacatta gaaaatgaaa ggggatttta tgcgtgagaa
     7021 tgttacagtc tatcccggca ttgccagtcg gggatattaa aaagagtata ggtttttatt
     7081 gcgataaact aggtttcact ttggttcacc atgaagatgg attcgcagtt ctaatgtgta
     7141 atgaggttcg gattcatcta tgggaggcaa gtgatgaagg ctggcgctct cgtagtaatg
     7201 attcaccggt ttgtacaggt gcggagtcgt ttattgctgg tactgctagt tgccgcattg
     7261 aagtagaggg aattgatgaa ttatatcaac atattaagcc tttgggcatt ttgcacccca
     7321 atacatcatt aaaagatcag tggtgggatg aacgagactt tgcagtaatt gatcccgaca
     7381 acaatttgat tagctttttt caacaaataa aaagctaaaa tctattatta atctgttcag
     7441 caatcgggcg cgattgctga ataaaagata cgagagacct ctcttgtatc ttttttattt
     7501 tgagtggttt tgtccgttac actagaaaac cgaaagacaa taaaaatttt attcttgctg
     7561 agtctggctt tcggtaagct agacaaaacg gacaaaataa aaattggcaa gggtttaaag
     7621 gtggagattt tttgagtgat cttctcaaaa aatactacct gtcccttgct gatttttaaa
     7681 cgagcacgag agcaaaaccc ccctttgctg aggtggcaga gggcaggttt ttttgtttct
     7741 tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg gttttggtcg gcactgccga
     7801 cagcctcgca gagcacacac tttatgaata taaagtatag tgtgttatac tttacttgga
     7861 agtggttgcc ggaaagagcg aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt
     7921 a
//
