Basic Vector Information
- Vector Name:
- pLVX-TetOne-Puro-Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10880 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- hPGK
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLVX-TetOne-Puro-Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLVX-TetOne-Puro-Luc vector Sequence
LOCUS 62056_16270 10880 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10880)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10880
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2027..2144
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
polyA_signal 2187..2321
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(2525..4174)
/label=luciferase
/note="firefly luciferase"
promoter complement(4181..4548)
/label=TRE3GS promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
promoter 4565..5075
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 5094..5837
/label=Tet-On(R) 3G
/note="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
promoter 5851..6180
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 6189..6785
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 6802..7390
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 7598..8231
/label=5' LTR
/note="5' long terminal repeat (LTR) from HIV-1"
promoter complement(8408..8438)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8453..8474)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8762..9347)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9521..10378)
/label=AmpR
/note="beta-lactamase"
promoter complement(10379..10483)
/label=AmpR promoter
polyA_signal 10531..10665
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
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