LOCUS       V014344                10880 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014344
VERSION     V014344
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2187..2321
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2525..4174)
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        complement(4181..4548)
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     promoter        4565..5075
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             5094..5837
                     /label="Tet-On(R) 3G"
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     promoter        5851..6180
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6189..6785
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6802..7390
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7598..8231
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8408..8438)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8453..8474)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8762..9347)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9521..10378)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10379..10483)
                     /label="AmpR promoter"
     polyA_signal    10531..10665
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
     2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
     2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
     2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
     2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
     2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
     2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
     2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
     2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
     3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
     3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
     3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
     3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
     3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
     3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
     3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
     3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
     3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
     3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
     3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
     3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
     3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
     3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
     3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
     3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
     3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
     4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
     4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
     4141 ccgggccttt ctttatgttt ttggcgtctt ccatgaattc tttacgaggg taggaagtgg
     4201 tacggaaagt tggtataaga caaaagtgtt gtggaattga agtttactca aaaaatcagc
     4261 actcttttat aggcgccctg gtttacataa gcaaagctta tacgttctct atcactgata
     4321 gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct
     4381 ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc
     4441 cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata
     4501 gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga ggtgataatt
     4561 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     4621 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     4681 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     4741 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     4801 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     4861 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     4921 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     4981 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     5041 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta
     5101 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
     5161 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     5221 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     5281 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     5341 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     5401 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     5461 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     5521 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     5581 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
     5641 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     5701 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     5761 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     5821 ttgacatgct ccccgggtaa acgcgcgaat gtgtgtcagt tagggtgtgg aaagtcccca
     5881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6001 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6181 acgcgaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     6241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6301 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6661 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6721 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6781 gtgcctgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7201 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7381 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     7441 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7501 aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7561 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     7621 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     7681 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     7741 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     7801 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     7861 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     7921 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     7981 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     8041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8221 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8281 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     8341 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8401 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8461 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8521 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10501 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10681 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
    10741 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10801 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
    10861 tgtactacaa acttagtagt
//
