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pOm45-PS-GFP(S65T)-TEF1-Zeo vector (V014226)

Basic Vector Information

Vector Name:
pOm45-PS-GFP(S65T)-TEF1-Zeo
Antibiotic Resistance:
Bleomycin
Length:
3218 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
Zeo
Promoter:
TEF1
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pOm45-PS-GFP(S65T)-TEF1-Zeo vector Vector Map

pOm45-PS-GFP(S65T)-TEF1-Zeo3218 bp6001200180024003000CYC1 terminatororiGFP (S65T)Myc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pOm45-PS-GFP(S65T)-TEF1-Zeo vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18285        3218 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      130..377
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1302..2012
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2020..2049
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2065..2082
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2162..2408
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2423..2834
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2842..2889
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             join(2908..3218,1..61)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"

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