LOCUS       62056_18285        3218 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      130..377
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1302..2012
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2020..2049
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2065..2082
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2162..2408
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2423..2834
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2842..2889
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             join(2908..3218,1..61)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
       61 ctgacacgtc cgacggcggc ccacgggtcc caggcctcgg agatccgtcc cccttttcct
      121 ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
      181 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
      241 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
      301 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
      361 cgaaggcttt aatttgcaag ctggagacca acatgtgagc aaaaggccag caaaaggcca
      421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
      661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
      901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatc agatctggcc caaaagaagt
     1141 acgatgaagc gttgaaaaag tacgatgaag ccaagaacaa attcaaagaa tggaatgata
     1201 agggtgatgg taaattctgg agctcgaaaa aggacggtgg tggtggtagc ggtggtggtg
     1261 gttcgggtgg tggtggtagc cggatccccg ggttaattaa cagtaaagga gaagaacttt
     1321 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1381 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1441 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc acttatggtg
     1501 ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     1561 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     1621 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     1681 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     1741 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     1801 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1861 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1921 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1981 ggattacaca tggcatggat gaactataca aatagtctag aacaaaaact catctcagaa
     2041 gaggatctga atagcgccgt cgaccatcat catcatcatc attgagtttg tagccttaga
     2101 catgactgtt cctcagttca agttgggcac ttacgagaag accggtcttg ctagattcta
     2161 atcaagagga tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt
     2221 tttatttgta acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag
     2281 cttgctcctg atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa
     2341 atcattcgag tttgatgttt ttcttggtat ttcccactcc tcttcagagt acagaagatt
     2401 aagtgagacc ttcgtttgtg cggatccccc acacaccata gcttcaaaat gtttctactc
     2461 cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc
     2521 caagcacagc atactaaatt ttccctcttt cttcctctag ggtgtcgtta attacccgta
     2581 ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc
     2641 aataaaaatt tttatcacgt ttctttttct tgaaattttt ttttttagtt tttttctctt
     2701 tcagtgacct ccattgatat ttaagttaat aaacggtctt caatttctca agtttcagtt
     2761 tcatttttct tgttctatta caactttttt tacttcttgt tcattagaaa gaaagcatag
     2821 caatctaatc taaggggcgg tgttgacaat taatcatcgg catagtatat cggcatagta
     2881 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     2941 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     3001 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     3061 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     3121 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     3181 ccggccatga ccgagatcgg cgagcagccg tgggggcg
//
