Basic Vector Information
- Vector Name:
- pMCS-Fluc-TK-Rluc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6136 bp
- Type:
- Luciferase reporter
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- HSV TK
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pMCS-Fluc-TK-Rluc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCS-Fluc-TK-Rluc vector Sequence
LOCUS 62056_16620 6136 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6136) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6136 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 100..1749 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" CDS 1756..1803 /codon_start=1 /label=hCL1 /note="non-ORF yeast peptide conferring ubiquitin-dependent degradation" /translation="ACKNWFSSLSHFVIHL" CDS 1807..1926 /codon_start=1 /label=hPEST /note="PEST degradation sequence from mouse ornithine decarboxylase" /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV" promoter 2099..2850 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 2920..3052 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 3097..3115 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3125..4057 /codon_start=1 /label=Rluc /note="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal complement(4091..4212) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 4345..4449 /label=AmpR promoter CDS 4450..5307 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5481..6069 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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