LOCUS       62056_16620        6136 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             100..1749
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1756..1803
                     /codon_start=1
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             1807..1926
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     promoter        2099..2850
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          2920..3052
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        3097..3115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3125..4057
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(4091..4212)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4345..4449
                     /label=AmpR promoter
     CDS             4450..5307
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5481..6069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctgcttg caagaactgg ttcagtagct taagccactt tgtgatccac
     1801 cttaacagcc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg
     1861 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc
     1921 aacgtctaac tgcagtctag agtcggggcg gccggccgct tcgagcagac atgataagat
     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagtttaaa
     2101 tgagtcttcg gacctcgcgg gggccgctta agcggtggtt agggtttgtc tgacgcgggg
     2161 ggagggggaa ggaacgaaac actctcattc ggaggcggct cggggtttgg tcttggtggc
     2221 cacgggcacg cagaagagcg ccgcgatcct cttaagcacc cccccgccct ccgtggaggc
     2281 gggggtttgg tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc
     2341 agagtgtgac cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg
     2401 ggctcgctgg gtcctaggct ccatggggac cgtatacgtg gacaggctct ggagcatccg
     2461 cacgactgcg gtgatattac cggagacctt ctgcgggacg agccgggtca cgcggctgac
     2521 gcggagcgtc cgttgggcga caaacaccag gacggggcac aggtacacta tcttgtcacc
     2581 cggaggcgcg agggactgca ggagcttcag ggagtggcgc agctgcttca tccccgtggc
     2641 ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc
     2701 tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca
     2761 gtcggggcgg cgcggtccca ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa
     2821 caccgagcga ccctgcagcg acccgcttaa aagcttgatt cttctgacac aacagtctcg
     2881 aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga
     2941 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc
     3001 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac
     3061 tcccagttca attacagctc ttaaggctag agtacttaat acgactcact ataggctagc
     3121 caccatgact tcgaaagttt atgatccaga acaaaggaaa cggatgataa ctggtccgca
     3181 gtggtgggcc agatgtaaac aaatgaatgt tcttgattca tttattaatt attatgattc
     3241 agaaaaacat gcagaaaatg ctgttatttt tttacatggt aacgcggcct cttcttattt
     3301 atggcgacat gttgtgccac atattgagcc agtagcgcgg tgtattatac cagaccttat
     3361 tggtatgggc aaatcaggca aatctggtaa tggttcttat aggttacttg atcattacaa
     3421 atatcttact gcatggtttg aacttcttaa tttaccaaag aagatcattt ttgtcggcca
     3481 tgattggggt gcttgtttgg catttcatta tagctatgag catcaagata agatcaaagc
     3541 aatagttcac gctgaaagtg tagtagatgt gattgaatca tgggatgaat ggcctgatat
     3601 tgaagaagat attgcgttga tcaaatctga agaaggagaa aaaatggttt tggagaataa
     3661 cttcttcgtg gaaaccatgt tgccatcaaa aatcatgaga aagttagaac cagaagaatt
     3721 tgcagcatat cttgaaccat tcaaagagaa aggtgaagtt cgtcgtccaa cattatcatg
     3781 gcctcgtgaa atcccgttag taaaaggtgg taaacctgac gttgtacaaa ttgttaggaa
     3841 ttataatgct tatctacgtg caagtgatga tttaccaaaa atgtttattg aatcggaccc
     3901 aggattcttt tccaatgcta ttgttgaagg tgccaagaag tttcctaata ctgaatttgt
     3961 caaagtaaaa ggtcttcatt tttcgcaaga agatgcacct gatgaaatgg gaaaatatat
     4021 caaatcgttc gttgagcgag ttctcaaaaa tgaacaataa ttctagagcg gccgcttcga
     4081 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     4141 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4201 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     4261 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca
     4321 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4381 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4441 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4501 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4561 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4621 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4681 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4741 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4801 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4861 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4921 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4981 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5041 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5101 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5161 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5221 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5281 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6061 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6121 gctcacatgg ctcgac
//
