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pPH-NBN vector (V014084)

Basic Vector Information

Vector Name:
pPH-NBN
Antibiotic Resistance:
Ampicillin
Length:
6974 bp
Type:
Protein expression
Replication origin:
ori
Host:
Insect cells
Promoter:
polyhedrin
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pPH-NBN vector Vector Map

pPH-NBN6974 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900f1 oriAmpR promoterAmpRoriTn7RGmRPc promoterpolyhedrin promoterNBNSV40 poly(A) signalTn7L

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPH-NBN vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18445        6974 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2513..2737)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3337)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3526..3554)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3906..3997
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4046..6307
                     /gene="NBN"
                     /label=NBN
                     /note="Nibrin from Homo sapiens. Accession#: O60934"
     polyA_signal    6464..6598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(6627..6792)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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