LOCUS       V014084                 6974 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014084
VERSION     V014084
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label="AmpR promoter"
     CDS             589..1446
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     mobile_element  complement(2513..2737)
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2807..3337)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(3526..3554)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        3906..3997
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4046..6307
                     /gene="NBN"
                     /label="Nibrin"
                     /note="Nibrin from Homo sapiens. Accession#: O60934"
     polyA_signal    6464..6598
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(6627..6792)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca gccgcgtaac
     2461 ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg
     2521 gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact
     2581 agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact
     2641 tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa
     2701 gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac aatttaccga
     2761 acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     2821 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     2881 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     2941 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     3001 ctgcgagatc atagatatag atctcactac gcggctgctc aaacctgggc agaacgtaag
     3061 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     3121 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     3181 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     3241 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     3301 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     3361 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     3421 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     3481 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     3541 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     3601 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     3661 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     3721 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     3781 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     3841 ttgttcgccc aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt
     3901 aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg
     3961 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
     4021 caccatcggg cgcggatccg ccaccatgtg gaaactgctg cccgccgcgg gcccggcagg
     4081 aggagaacca tacagacttt tgactggcgt tgagtacgtt gttggaagga aaaactgtgc
     4141 cattctgatt gaaaatgatc agtcgatcag ccgaaatcat gctgtgttaa ctgctaactt
     4201 ttctgtaacc aacctgagtc aaacagatga aatccctgta ttgacattaa aagataattc
     4261 taagtatggt acctttgtta atgaggaaaa aatgcagaat ggcttttccc gaactttgaa
     4321 gtcgggggat ggtattactt ttggagtgtt tggaagtaaa ttcagaatag agtatgagcc
     4381 tttggttgca tgctcttctt gtttagatgt ctctgggaaa actgctttaa atcaagctat
     4441 attgcaactt ggaggattta ctgtaaacaa ttggacagaa gaatgcactc accttgtcat
     4501 ggtatcagtg aaagttacca ttaaaacaat atgtgcactc atttgtggac gtccaattgt
     4561 aaagccagaa tattttactg aattcctgaa agcagttgag tccaagaagc agcctccaca
     4621 aattgaaagt ttttacccac ctcttgatga accatctatt ggaagtaaaa atgttgatct
     4681 gtcaggacgg caggaaagaa aacaaatctt caaagggaaa acatttatat ttttgaatgc
     4741 caaacagcat aagaaattga gttccgcagt tgtctttgga ggtggggaag ctaggttgat
     4801 aacagaagag aatgaagaag aacataattt ctttttggct ccgggaacgt gtgttgttga
     4861 tacaggaata acaaactcac agaccttaat tcctgactgt cagaagaaat ggattcagtc
     4921 aataatggat atgctccaaa ggcaaggtct tagacctatt cctgaagcag aaattggatt
     4981 ggcggtgatt ttcatgacta caaagaatta ctgtgatcct cagggccatc ccagtacagg
     5041 attaaagaca acaactccag gaccaagcct ttcacaaggc gtgtcagttg atgaaaaact
     5101 aatgccaagc gccccagtga acactacaac atacgtagct gacacagaat cagagcaagc
     5161 agatacatgg gatttgagtg aaaggccaaa agaaatcaaa gtctccaaaa tggaacaaaa
     5221 attcagaatg ctttcacaag atgcacccac tgtaaaggag tcctgcaaaa caagctctaa
     5281 taataatagt atggtatcaa atactttggc taagatgaga atcccaaact atcagctttc
     5341 accaactaaa ttgccaagta taaataaaag taaagatagg gcttctcagc agcagcagac
     5401 caactccatc agaaactact ttcagccgtc taccaaaaaa agggaaaggg atgaagaaaa
     5461 tcaagaaatg tcttcatgca aatcagcaag aatagaaacg tcttgttctc ttttagaaca
     5521 aacacaacct gctacaccct cattgtggaa aaataaggag cagcatctat ctgagaatga
     5581 gcctgtggac acaaactcag acaataactt atttacagat acagatttaa aatctattgt
     5641 gaaaaattct gccagtaaat ctcatgctgc agaaaagcta agatcaaata aaaaaaggga
     5701 aatggatgat gtggccatag aagatgaagt attggaacag ttattcaagg acacaaaacc
     5761 agagttagaa attgatgtga aagttcaaaa acaggaggaa gatgtcaatg ttagaaaaag
     5821 gccaaggatg gatatagaaa caaatgacac tttcagtgat gaagcagtac cagaaagtag
     5881 caaaatatct caagaaaatg aaattgggaa gaaacgtgaa ctcaaggaag actcactatg
     5941 gtcagctaaa gaaatatcta acaatgacaa acttcaggat gatagtgaga tgcttccaaa
     6001 aaagctgtta ttgactgaat ttagatcact ggtgattaaa aactctactt ccagaaatcc
     6061 atctggcata aatgatgatt atggtcaact aaaaaatttc aagaaattca aaaaggtcac
     6121 atatcctgga gcaggaaaac ttccacacat cattggagga tcagatctaa tagctcatca
     6181 tgctcgaaag aatacagaac tagaagagtg gctaaggcag gaaatggagg tacaaaatca
     6241 acatgcaaaa gaagagtctc ttgctgatga tctttttaga tacaatcctt atttaaaaag
     6301 gagaagagtc gactagctcg aggcatgcgg taccaagctt gtcgagaagt actagaggat
     6361 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     6421 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     6481 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6541 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     6601 atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc caaactattt
     6661 tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc
     6721 actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt
     6781 tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa
     6841 ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt
     6901 ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc
     6961 tgaatggcga atgg
//
