pKM24KHibm8 vector (V005205)

LOCUS       40924_26909       14226 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKM24KHibm8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Sahoo DK, Raha S, Hall JT, Maiti IB.
  TITLE     Overexpression of the synthetic chimeric native-T-phylloplanin-GFP 
            genes optimized for monocot and dicot plants renders enhanced 
            resistance to blue mold disease in tobacco (N. tabacum L.)
  JOURNAL   ScientificWorldJournal 2014, 601314 (2014)
  PUBMED    24778589
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Maiti IB, Sahoo DK, Raha S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2013) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14226)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ScientificWorldJournal 2014, 601314 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2013) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..641
                     /note="M24 promoter from Mirabilis Mosaic Virus (MMV), Ref:
                     US Patent No. 64205470; Plant Mol. Biol. 40: 771-782 
                     (1999); Transgenics 3: 61-70 (1999)"
                     /regulatory_class="promoter"
     regulatory      642..691
                     /note="5'-untranslated region of Alfalfa mosaic virus
                     (AlMV) RNA 4. ref: Nature 325: 622-625(1987)"
                     /regulatory_class="enhancer"
     sig_peptide     692..760
                     /gene="fused T-phylloplanin-GFP"
                     /note="apoplast/cell wall/plasma membrane targeting protein
                     sequence (aTPS) of Arabidopsis 2S2 protein gene. Ref: Plant
                     Physiol. 155: 2036-2048 (2011); Proc Natl Acad Sci, USA, 
                     104: 1430-1435 (2007)."
     misc_feature    761..1213
                     /gene="fused T-phylloplanin-GFP"
                     /note="synthetic T-phylloplanin sequence, (GenBank
                     accession No. AY705384), full-length with its own signal 
                     peptide, is optimized for dicot plant (tobacco) bias 
                     codons"
     misc_feature    1214..1219
                     /gene="fused T-phylloplanin-GFP"
                     /note="linker sequence used to fuse T-phylloplanin and GFP
                     genes in frame"
     CDS             1226..1936
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     terminator      1961..2602
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        2691..2870
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2898..3917
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      3929..4178
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5069..5887)
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     promoter        complement(5888..6071)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6227..6251
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6731..7440
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8437..9084)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             9190..10386
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(11665..12810)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(13085..13453)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(13486..13595)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    14150..14174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"