LOCUS       40924_26909       14226 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKM24KHibm8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Sahoo DK, Raha S, Hall JT, Maiti IB.
  TITLE     Overexpression of the synthetic chimeric native-T-phylloplanin-GFP 
            genes optimized for monocot and dicot plants renders enhanced 
            resistance to blue mold disease in tobacco (N. tabacum L.)
  JOURNAL   ScientificWorldJournal 2014, 601314 (2014)
  PUBMED    24778589
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Maiti IB, Sahoo DK, Raha S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2013) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14226)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ScientificWorldJournal 2014, 601314 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-DEC-2013) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..641
                     /note="M24 promoter from Mirabilis Mosaic Virus (MMV), Ref:
                     US Patent No. 64205470; Plant Mol. Biol. 40: 771-782 
                     (1999); Transgenics 3: 61-70 (1999)"
                     /regulatory_class="promoter"
     regulatory      642..691
                     /note="5'-untranslated region of Alfalfa mosaic virus
                     (AlMV) RNA 4. ref: Nature 325: 622-625(1987)"
                     /regulatory_class="enhancer"
     sig_peptide     692..760
                     /gene="fused T-phylloplanin-GFP"
                     /note="apoplast/cell wall/plasma membrane targeting protein
                     sequence (aTPS) of Arabidopsis 2S2 protein gene. Ref: Plant
                     Physiol. 155: 2036-2048 (2011); Proc Natl Acad Sci, USA, 
                     104: 1430-1435 (2007)."
     misc_feature    761..1213
                     /gene="fused T-phylloplanin-GFP"
                     /note="synthetic T-phylloplanin sequence, (GenBank
                     accession No. AY705384), full-length with its own signal 
                     peptide, is optimized for dicot plant (tobacco) bias 
                     codons"
     misc_feature    1214..1219
                     /gene="fused T-phylloplanin-GFP"
                     /note="linker sequence used to fuse T-phylloplanin and GFP
                     genes in frame"
     CDS             1226..1936
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     terminator      1961..2602
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        2691..2870
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2898..3917
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      3929..4178
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5069..5887)
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     promoter        complement(5888..6071)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6227..6251
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6731..7440
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8437..9084)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             9190..10386
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(11665..12810)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(13085..13453)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(13486..13595)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    14150..14174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaattcgtca acttcgtcca cagacatcaa catcttatcg tcctttgaag ataagataat
       61 aatgttgaag ataagagtgg gagcccccac taaaacattg ctttgtcaaa agctaaaaaa
      121 gatgatgccc gacagccact tgtgtgaagc atgagaagcc ggtccctcca ctaagaaaat
      181 tagtgaagca tcttccagtg gtccctccac tcacagctca atcagtgagc aacaggacga
      241 aggaaatgac gtaagccatg acgtctaatc ccaacttcgt ccacagacat caacatctta
      301 tcgtcctttg aagataagat aataatgttg aagataagag tgggagccac cactaaaaca
      361 ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc acttgtgtga agcatgagaa
      421 gccggtccct ccactaagaa aattagtgaa gcatcttcca gtggtccctc cactcacagc
      481 tcaatcagtg agcaacagga cgaaggaaat gacgtaagcc atgacgtcta atcccacaag
      541 aatttcctta tataaggaac acaaatcaga aggaagagat caatcgaaat caaaatcgga
      601 atcgaaatca aaatcggaat cgaaatctct catctaagct tggatcctcg agcggatctt
      661 tttattttta attttctttc aaatacttcc aatgggagct aacaagttgt tccttgtgtg
      721 cgctactttt gctctttgct tccttcttac taacgctccc atgggtgcta gtgctaagat
      781 tttccttatt ttcttgcttg ctgctcttat tgctactcca gctgctttcg ctattcttgt
      841 gccaactctt gtgtctactc acatttctgg acttgtgttc tgctctgtga acggaaacct
      901 tgatgtgatt aacggacttt ctccacaggt tttcccaaac gcttctgttc aacttagatg
      961 cggagctact aacgtgattt cttctactat tactaacgga tctggtgctt tctctcttgc
     1021 tgtgaacact ttcccacttt tgaactgcaa ccttgtggtt gctactccac tttctacttg
     1081 caacgctact cttcagtctg ttggaaggct tgcttcttct cttaggcttg tgaacattac
     1141 tcttggatct ggaactggac ttattagagt gggacttgct ccaactggat tcattcttaa
     1201 ccttaacatt aacgtcgaca tgggatctaa gggtgaagag cttttcactg gtgttgtgcc
     1261 tattcttgtg gagcttgatg gtgatgttaa cggacacaag ttctctgtta gtggagaggg
     1321 tgaaggtgat gctacttacg gaaagttgac tcttaagttc atctgcacta ctggaaagtt
     1381 gccagttcct tggcctactt tggtgactac tttctcttac ggtgttcagt gcttctctag
     1441 gtatccagat cacatgaagc agcacgattt cttcaagtct gctatgccag agggatatgt
     1501 gcaagagagg actattttct tcaaggatga tggaaactac aagactaggg ctgaggttaa
     1561 gttcgagggt gatactcttg tgaacaggat tgagcttaag ggaattgatt tcaaagagga
     1621 tggaaacatt cttggacaca agttggagta caactacaac tctcacaacg tgtacattat
     1681 ggctgataag cagaagaacg gtattaaggt taacttcaaa atcaggcata acattgagga
     1741 tggatctgtt cagcttgctg atcactacca gcagaacact cctattggtg atggaccagt
     1801 gcttcttcca gataaccact acctttctac tcagtctgct ctttctaagg atccaaacga
     1861 gaagagggat cacatggtgc ttttggagtt cgttactgct gctggaatta ctcacggaat
     1921 ggatgagctt tacaagtgag agctcgaagt gatcctctag agctttcgtt cgtatcatcg
     1981 gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct
     2041 actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt
     2101 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg
     2161 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt
     2221 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt
     2281 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca
     2341 cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa
     2401 agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt
     2461 cctttatgta attttccaga atccttgtca gattctaatc attgctttat aattatagtt
     2521 atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca
     2581 attgattgac aacatgcatc aatcgatgat catgagcgga gaattaaggg agtcacgtta
     2641 tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca gaaccgcaac
     2701 gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca catacgtcag
     2761 aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca aatatttctt
     2821 gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta gagtctcata
     2881 ttcactctca agatatcatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     2941 tgatcgaaaa gttcgacagc gtctccgacc agatgcagct ctcggagggc gaagaatctc
     3001 gtgctttcag cttcgatgta agagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     3061 atggttccta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     3121 cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtt
     3181 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg
     3241 tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     3301 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     3361 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     3421 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     3481 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa cggccgcata acagcggtca
     3541 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     3601 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     3661 agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct
     3721 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     3781 caatcgtccg atccggagcc gggactgtcg ggcgaacaca aatcgcccgc agaagcgcgg
     3841 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     3901 ctcgtccgag ggcaaaggga tagggatcca tcgttcaaac atttggcaat aaagtttctt
     3961 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     4021 taagcatgta ataattaaca tgtaatgcat gacgttgttt atgagatggg tttttatgat
     4081 tagagtcccg caattataca tttaatacac gatagaaaac aaaatatagc gcgcaaacta
     4141 ggataaatta tcgcgcgcgg tgtcatctat gttactagta atcgatcgat ctagtaacat
     4201 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     4261 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     4321 gcattacatg ttaattatta catgcttaac gtaatcaaca gaaattatat gataatcatc
     4381 gcaagaccgg caacaggatt caattttaag aaactttatt gcccaaatgg ttttgacgat
     4441 ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa cgtgggtagc
     4501 accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg atttgccagt
     4561 tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg tgaggggtat
     4621 ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata attcttcaga
     4681 aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag gtgacggtgc
     4741 taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc attcctgcaa
     4801 gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt ggtctatttg
     4861 ctcttggacg ttgcaaacgt aagtgttgga tcggggtggg cgaagaactc cagcatgaga
     4921 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     4981 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     5041 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     5101 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     5161 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     5221 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     5281 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     5341 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     5401 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     5461 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     5521 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     5581 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     5641 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     5701 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     5761 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     5821 cggccggaga acctgcccgg atccgggcgg aaataggtaa agaagttgcg gataaggtaa
     5881 ttgccattgc agattatttg gattgagagt gaatatgaga ctctaattgg ataccgaggg
     5941 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     6001 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     6061 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta
     6121 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc
     6181 atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
     6241 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     6301 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     6361 agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt cggaaagcct
     6421 gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct agaaggtcga
     6481 gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat
     6541 cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga
     6601 cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc gcgagttgct
     6661 ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca
     6721 agatcgtcga ccctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc
     6781 cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg
     6841 ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga
     6901 cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg
     6961 atttcggccg gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta
     7021 cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca
     7081 cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag
     7141 tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc
     7201 cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc
     7261 ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc
     7321 gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc
     7381 tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag
     7441 ccaagctagc ttgggctgaa attgcaggac aactggtgag tctggatctg aagttcattg
     7501 ttgtcatgga tgatctggac cgactggagc catcccaggt ggcggaagtg ttcaggcttg
     7561 tgcgtgcagt agccgatctg ccccgcttta cccatattct ctgttatgac aggcagatta
     7621 tcactcatgc cgttgaacat gcgctgaata tcgaagatgg cagccgttat ctccagaaaa
     7681 tcattcagct tagttttaaa ttaccccgac ctgaagcctt tgatttacgt aatgaatttc
     7741 gccagcgggc tgaggctcta tatcagcaaa ttaataatca accgccagac tctggaatgg
     7801 taagggatct catcgcggtg actgatacct atggtgccgc actttcgacg ccacgggaaa
     7861 tccatcaggc cattaattcc ctgatttttc tttatccggg gatgcgggat tttgtttatt
     7921 tccctgattt gtgcctgctt cagcttatac gggtgacaaa cccggctctg tatgactgga
     7981 cagagcatta cctgacagaa cggtccgtga ttgaaaccgg tcagggtatg ctttctgacg
     8041 gagagaaagc agacttccgg gaggggctta tcagatgtat gaagacgttc agggcatcaa
     8101 atgcagactc gtttctgaca cttgcagact ggatctatct catctgcgca aggcagaacg
     8161 tgaagacggc cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga
     8221 cccgcgccac ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg
     8281 ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct
     8341 gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt
     8401 agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc
     8461 aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg
     8521 ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg
     8581 cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg
     8641 ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc
     8701 cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg
     8761 cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc
     8821 ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg
     8881 caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg
     8941 cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt
     9001 gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac
     9061 tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc
     9121 accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg
     9181 aggccagaca tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac
     9241 gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac
     9301 tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt
     9361 gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc
     9421 gtctcgctgg ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg
     9481 gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc
     9541 gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc
     9601 gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc
     9661 tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc
     9721 tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc
     9781 aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg
     9841 gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc
     9901 gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt
     9961 ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg
    10021 cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg
    10081 acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg
    10141 ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa
    10201 ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg
    10261 atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc
    10321 tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc
    10381 gatcgctgat cgtggaaacg ataggcctat gccatgcggg tcaaggcgac ttccggcaag
    10441 ctatacgcgc cctaggagtg cggttggaac gttggcccag ccagatactc ccgatcacga
    10501 gcaggacgcc gatgatttga agcgcactca gcgtctgatc caagaacaac catcctagca
    10561 acacggcggt ccccgggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg
    10621 agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca
    10681 ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg
    10741 gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg
    10801 gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc
    10861 ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc
    10921 ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag
    10981 cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc
    11041 ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc
    11101 cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga
    11161 gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt
    11221 cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct
    11281 gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat
    11341 ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc
    11401 cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt
    11461 cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt
    11521 ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg
    11581 tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc
    11641 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
    11701 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
    11761 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
    11821 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
    11881 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
    11941 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
    12001 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
    12061 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
    12121 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
    12181 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
    12241 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
    12301 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
    12361 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
    12421 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
    12481 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
    12541 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
    12601 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
    12661 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
    12721 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
    12781 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
    12841 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
    12901 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
    12961 ggtattccga atcttgccct gcacgaatac cagctccgcg aagtcgctct tcttgatgga
    13021 gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa
    13081 gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct
    13141 tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg
    13201 ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc
    13261 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga
    13321 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc
    13381 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg
    13441 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
    13501 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
    13561 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
    13621 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
    13681 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag atccgtcgac
    13741 gatcgtcaac gttcacttct aaagaaatag cgccactcag cttcctcagc ggctttatcc
    13801 agcgatttcc tattatgtcg gcatagttct caagatcgac agcctgtcac ggttaagcga
    13861 gaaatgaata agaaggctga taattcggat ctctgcgagg gagatgatat ttgatcacag
    13921 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    13981 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    14041 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    14101 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    14161 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    14221 tgtact
//
