pJV53-GFP vector (V005284)

LOCUS       40924_26366        9885 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJV53-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Efficient and simple generation of multiple unmarked gene deletions 
            in Mycobacterium smegmatis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) Microbiology, Institute of Pathogen 
REFERENCE   3  (bases 1 to 9885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2015) Microbiology, Institute of Pathogen Biology,Chinese 
            Academy of Medical Sciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3334..3380
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             3541..4353
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4865..5578
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"