LOCUS       40924_26366        9885 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJV53-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Efficient and simple generation of multiple unmarked gene deletions 
            in Mycobacterium smegmatis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Mao X-J., Yan M-Y., Zhu H, Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) Microbiology, Institute of Pathogen 
REFERENCE   3  (bases 1 to 9885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2015) Microbiology, Institute of Pathogen Biology,Chinese 
            Academy of Medical Sciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3334..3380
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             3541..4353
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             4865..5578
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      5653..6241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgcatgaccg ccggcgggtg ctcgtggcgt tgaccccgcg ggggcggacg ctgcgcaacc
       61 gcctggtgga cgcggtagcc gaggccgagt gtgcggcttt tgaatcgtgc gggctggacg
      121 tcgaccagtt gcgcgaactc gtcgacacca cctcgaattt gacttcgtaa ccacccgcgc
      181 ccggcgcggg cgttcaccct tgacttttat tttcatctgg atatatttcg ggtgaatgga
      241 aaggggtgac catgccgacc tacacattcc gttgttccca ctgcggtccc ttcgatctca
      301 cctgcgcgat ctccgagcgc gatgcggcgg cgacctgtcc ggagtgccgg acgccggcgc
      361 gccgggtctt cggttcggta gggctgacga cattcaccgc gggacatcac cgcgcattcg
      421 acgcggcgtc cgcgagcgcc gaaagtccca cggtggtgaa gtcgattccc gcaggcgcgg
      481 accgcccgcg ggccccgcgc cgcaatcccg gtctaccgag tctgccgagg tactagcgac
      541 atgggtggcg tcgggctctt ctacgtgggt gcggtgctca tcatcgacgg gctgatgctg
      601 ctgggccgca tcagcccacg aggcgcaaca ccgctgaact tcttcgtcgg cggactgcag
      661 gtggtgacgc ctacggtgct gatcctgcag tccggcggag acgcggccgt gatcttcgcg
      721 gcctccgggc tctacctgtt cggcttcacc tacctgtggg tggccatcaa caacgtgacc
      781 gactgggacg gagaaggtct cggatggttc tcgctgttcg tcgcgatcgc cgcactcggc
      841 tactcgtggc acgcgttcac cgccgaggcc gacccggcgt tcggggtgat ctggctgctg
      901 tgggcagtgc tgtggttcat gctgttcctg ctgctcggcc tggggcacga cgcactgggg
      961 cccgccgtcg ggttcgtcgc ggtggccgaa ggcgtgatca ccgccgccgt gccggccttc
     1021 ctgatcgtgt cgggcaactg ggaaaccggc ccgctccccg ccgcggtcat cgccgtgatc
     1081 ggttttgccg cagttgttct cgcatacccc atcgggcgcc gtctcgcagc gccgtcagtc
     1141 accaaccctc caccggccgc gctcgcggcc accacccgat aagagaaagg gagtccacat
     1201 atgtaacgga tccagctgca gaattcgaag cttatcgatg tcgacgtagt tacgagatcg
     1261 gcggccgcat atgagtgtgc ccacacagga cggaatgcac cggttcgtcg acgaggacgt
     1321 ctaccacgct gaccggggct cgctgtcggt atccggcgcg aagctgctgt tgccgccgtc
     1381 gtgtcccgcg aaattccgct gggagatgga caacacccgg aagccgaaaa aggtctggga
     1441 cttcggacat gtcgcgcaca aactggtgct cggcaagggt gccgagttcg agatcctcga
     1501 ccccgaggtg cacgggctga aggcggacgg tacgccgtcg gagaagccga ccgcgacggg
     1561 catgtggcgc aaggccgagg ctgaggctcg caaacagggc aaggtgccga ttcacgtcga
     1621 cctgttcacg aaggcgtacg acatggccga aaaggtgcgt cagcacccga cagccggccc
     1681 gatcttcgcc aatcctgacg gcgaggccga ggtcgcgctg tactacaccg accccgagac
     1741 cggcgtgcgg ctgcgtggcc ggatcgactg gctcactgac gatatcgatg attacaagac
     1801 gtcgatgacc gcgaacccgg ccgagctgaa aaccaagttc tacaagctcg gctatttcat
     1861 gcaggcggcc tggtacatcg atctactggt cgccctcggg ctcgccgaga acccgcgatt
     1921 ccggttcatc acgcaggaga aagaaccgcc ctacgtcgtg actccgatcc agtacgacga
     1981 cgaggcgatc gaagaggggc ggcgccgcaa ccgccaggcg atccggctct acgccgactg
     2041 catggaatcg ggcaagtggc ctgactacag cgacgacgtg gtcacgatca gcctgccctc
     2101 gtgggggctg ccgcgaccgc agaccgtcgg cgacgtcgtc accgacagct atatctacga
     2161 caccgacccg ctcgaagagg ccgacccgat tgaaggggat tacatctatg gctgaaaatg
     2221 ctgtcaccaa gcaggattcg cccaaggcac ccgagacgat ctcgcaagtg ctgcaggtgc
     2281 tcgtgccgca gctggcgcgt gcagtgccca agggtatgga ccccgaccgc atcgcgcgga
     2341 tcgtgcaaac cgagatccgc aagtcgcgca acgcgaaagc tgctggcatc gctaagcagt
     2401 ccctcgacga ctgcacgcaa gagtcatttg ccggtgcgct gctgacctcg gccgcgctcg
     2461 gtctcgagcc cggtgtcaac ggcgagtgct acctcgtgcc ctaccgcgac acccggcgcg
     2521 gcgtggtcga gtgccagctg atcatcggct accagggcat cgtcaaactg ttctggcagc
     2581 acccgcgcgc ctcccggatc gacgcgcagt gggtcggcgc gaacgacgaa ttccattaca
     2641 ccatgggcct caatccgacg ctgaaacacg tgaaggccaa gggtgatcgg ggtaatccgg
     2701 tctacttcta cgcgatcgtc gaggtgaccg gcgctgagcc gctgtgggac gtgttcaccg
     2761 ccgacgagat cagggaattg cgtcgcggca aggtcggatc ctcgggcgac atcaaggacc
     2821 cgcagcgctg gatggagcgg aagaccgcgc tcaaacaggt gctgaagctg gcaccgaaga
     2881 cgacgcggct cgacgcggcg atccgcgccg acgatcgccc gggcaccgac ctgtcacagt
     2941 cgcaggcgct cgcgctgccg tcgaccgtca agccgacggc cgactacatc gacggcgaga
     3001 tcgccgagcc gcacgaggtc gatacgccac cgaagtcgtc gcgcgcacaa cgcgcgcagc
     3061 gcgccaccgc gccggcgccc gacgtgcaga tggccaatcc cgatcagctg aagcgcctcg
     3121 gcgagatcca gaaggccgaa aagtacaacg acgccgattg gttcaagttc ctcgccgatt
     3181 cggccggcgt caaagccacg cgcgccgccg acctcacgtt cgacgaggcg aaggctgtca
     3241 tcgacatgtt cgacgggccc aacgcatgag cgccccggcg aattccgacg cggtggttga
     3301 tctgcaacta gcgtacgatc gactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     3361 gactgggcct ttcgttttat gccatcatgg ccgcggtgat cagctagcca cctgacgtcg
     3421 gggggggggg aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
     3481 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     3541 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
     3601 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     3661 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     3721 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     3781 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     3841 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     3901 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     3961 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     4021 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     4081 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     4141 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     4201 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     4261 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     4321 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     4381 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     4441 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     4501 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcacc aaccgtggct
     4561 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     4621 cttcacgagg cagacctcac tagtttgatc tgtgcgttcg cacgcacagg cccggtgtga
     4681 gaagggtctc tgacgagcgg gagaacccac ccggggtggg cgagtttgtc ctgcgtgtgc
     4741 tcggtcgagt aggctctggg agtacccgtg tgtacgacca gcacggcata catcatttcg
     4801 acgccgagag attcgccgcc cgaaatgagc acgatccgca tgcttaacag aaaggaggtt
     4861 aataatgtcg aagggcgagg agctgttcac cggcgtcgtc ccgatcctgg tcgagctgga
     4921 cggtgacgtc aacggccaca agttctccgt ctccggcgag ggtgagggcg acgccaccta
     4981 cggcaagctg accctgaagt tcatctgcac caccggtaag ctgccggtcc cgtggccgac
     5041 cctggtcacc accctgacct acggcgtcca gtgcttctcc cgctacccgg accacatgaa
     5101 gcgccacgac ttcttcaagt ccgccatgcc ggagggttac gtccaggagc gcaccatctc
     5161 cttcaaggac gacggtaact acaagacgcg tgccgaggtc aagttcgagg gcgacaccct
     5221 ggtcaaccgc atcgagctga agggcatcga cttcaaggag gacggtaaca tcctgggcca
     5281 caagctggag tacaactaca actcccacaa cgtctacatc accgcggaca agcagaagaa
     5341 cggcatcaag gccaacttca agacccgcca caacatcgag gacggtggcg tccagctagc
     5401 cgaccactac cagcagaaca ccccgatcgg cgacggcccg gtcctgctgc cggacaacca
     5461 ctacctgtcc acccagtccg ccctgtccaa ggacccgaac gagaagcgcg accacatggt
     5521 cctgctggag ttcgtcaccg ccgccggcat cacccacggc atggacgagc tgtacaagta
     5581 gatttatcac cagcccgtca tcgtactagt tccatgagcg tcagaccccg tagaaaagat
     5641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5761 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6061 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6361 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgagcccacc agctccgtaa
     6421 gttcgggcgc tgtgtggctc gtacccgcgc attcaggcgg cagggggtct aacgggtcta
     6481 aggcggcgtg tacggccgcc acagcggctc tcagcggccc ggaaacgtcc tcgaaacgac
     6541 gcatgtgttc ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg ctggtgttcc
     6601 accaccaggg ctcgacggga gagcggggga gtgtgcagtt gtggggtggc ccctcagcga
     6661 aatatctgac ttggagctcg tgtcggacca tacaccggtg attaatcgtg gtctactacc
     6721 aagcgtgagc cacgtcgccg acgaatttga gcagctctgg ctgccgtact ggccgctggc
     6781 aagcgacgat ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc taggccgccg
     6841 gtacatcgag gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg tagaccatcc
     6901 agacgcagcg ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca acgcgatcgt
     6961 gggcaatcgc gccaacggcc acgcacacgc agtgtgggca ctcaacgccc ctgttccacg
     7021 caccgaatac gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg aaggccttcg
     7081 gcgcgccgtc gatggcgacc gcagttactc aggcctcatg accaaaaacc ccggccacat
     7141 cgcctgggaa acggaatggc tccactcaga tctctacaca ctcagccaca tcgaggccga
     7201 gctcggcgcg aacatgccac cgccgcgctg gcgtcagcag accacgtaca aagcggctcc
     7261 gacgccgcta gggcggaatt gcgcactgtt cgattccgtc aggttgtggg cctatcgtcc
     7321 cgccctcatg cggatctacc tgccgacccg gaacgtggac ggactcggcc gcgcgatcta
     7381 tgccgagtgc cacgcgcgaa acgccgaatt tccgtgcaac gacgtgtgtc ccggaccgct
     7441 accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca caaccaagtc
     7501 gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc gccagtcggc
     7561 catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc ggcgcgcaaa
     7621 gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg ctacaaccgg
     7681 cagccgactg tccgcaaaaa gcggcgcgtg accgccgccg aaggcgctcg aatcaccgga
     7741 ctatccgaac gccacgtcgt ccggctcgtg gcgcaggaac gcagcgagtg gctcgccgag
     7801 caggctgcac gccgcgaacg catccgcgcc tatcacgacg acgagggcca ctcttggccg
     7861 caaacggcca aacatttcgg gctgcatctg gacaccgtta agcgactcgg ctatcgggcg
     7921 aggaaagagc gtgcggcaga acaggaagcg gctcaaaagg cccacaacga agccgacaat
     7981 ccaccgctgt tctaacgcaa ttggggagcg ggtgtcgcgg gggttccgtg gggggttccg
     8041 ttgcaacggg tcggacaggt aaaagtcctg gtagacgcta gttttctggt ttgggccatg
     8101 cctgtctcgt tgcgtgtttc gttgcgcccg ttttgaatac cagccagacg agacggggtt
     8161 ctacgaatct tggtcgatac caagccattt ccgctgaata tcggggagct caccgccaga
     8221 atcggtggtt gtggtgatgt acgtggcgaa ctccgttgta gtgcctgtgg tggcatccgt
     8281 ggcgcggccg cggtaccaga tctttaaatc tagataaaga agtgacgcgg tctcaagcgt
     8341 cgagcgtcgc cagcgtgtcg aggatgtcga agtcgtagcc gtcggcgctg gcgatgtaga
     8401 cctgctggtc gaattgactg tcgcgcatac acatcgggcc ccggggcccg tcgaacccga
     8461 catcgtgcgc ggatgccatc aggtccggta tctcggggga gtgggcccgc tggaagatgg
     8521 cctcgagcgc aagcagaccc tcgtaacagg attcggccat cgcgttgagc ggtggcgcgt
     8581 cggcgccgta gcgggcgacg tagctgccca tcaggtccat ggcacccgcg gtggccagtg
     8641 aactgaagta cgccgcggcg acatagaggt tttcggtgga gccggcgccg ctggccagca
     8701 gcatgttctc ctccatcagc gggctgaacc gcgccatgcg gtcgtgcccg ccggcgcgcg
     8761 cgaactcgcg gttgaacaac acggcgtcct ggccgacgag cagcatcaac acggcctgcg
     8821 cccccgacgc gatggccttg cggacaggtg cgcggaaatc gtcggtgccg tacgggacgt
     8881 agatctcccg tctgagctcg aggtccagat ctcggcagta cgcgcgggcg gccgcggcgg
     8941 aacggcgcgg ccagatgtag tcatcgccga ccaggcacca ggaccggatg ccgaagtggt
     9001 cgcgcagcca ggcgagcgcg ggcgcgatct ggatctgcgg tgtctcgcct gtgcagaaca
     9061 cgcccggtgt gcgttcaccg ccctcgtaca acgaggtgta gacgtacggg atgcggtcgc
     9121 ggaccaccgg ggagatgcgg ttgcgcacgg ccgagatgtg ccagccggtc acggcgtcga
     9181 gaccgtgacc tcgcaaccgg tcggcgacgg tccgggcgac gtcgtcgccg ggcgctccgc
     9241 cgtcgagcac ctcgatggtg accttgcggc cctgcaggcc gcctcggtcg ttgacctcct
     9301 tggccgcgag ctcggccacg gcctcgcacg aaggcgcgaa gattcccgct ggcccttgaa
     9361 gcggaatcac cagcccgacg cggaactcaa cctcgccgtc ctgcactcca gatcaccgtc
     9421 gatcccgtgt agtctgcgct tcaaagcttt ctagcagaaa taattcattc tgaacagacc
     9481 ccgccgtcga cacgaggaga cacccaccat ggccgccgga cagcagcgcc gccccaacct
     9541 cctgctgccg ttggtgcgtc tgacccacct cgcggagtcg gcgatcgaac gcgtgctcgc
     9601 ggactcgtcg ctcaagatcg aggactggcg cgtgctcgac gagttggccg gacggcgcac
     9661 cgtgcccatg agcgatctcg cgcaggccac gctgatcacg ggtccgactc tcaccagaac
     9721 cgtcgatcgc cttgtgtcgc aagggatcat ctaccggact gccgatctgc atgaccgccg
     9781 gcgggtgctc gtggcgttga ccccgcgggg gcggacgctg cgcaaccgcc tggtggacgc
     9841 ggtagccgag gccgagtgtg cggctttgaa tcgtgcgtct gacga
//
