pJL71-2W-gpmcherry vector (V005310)

LOCUS       V005310                 7279 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005310
VERSION     V005310
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Scharschmidt TC, Rosenblum MD.
  TITLE     A wave of regulatory T cells into neonatal skin mediates tolerance
            to commensal microbes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Scharschmidt TC, Rosenblum MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2014) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 7279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-OCT-2014) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..306
                     /regulatory_class="terminator"
     CDS             2837..3568
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        3936..4040
                     /label="AmpR promoter"
     CDS             4041..4898
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5072..5660
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5761..6165)
                     /codon_start=1
                     /product="ArgB"
                     /label="ArgB"
                     /protein_id="AKT95053.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLIV
                     MYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNFHI
                     NFLIMIILTVISLGVISVYAPSGRQLTQRR"
     regulatory      5792..6479
                     /gene="agr"
                     /regulatory_class="promoter"
     gene            5792..6479
                     /gene="agr"
                     /label="agr"
     regulatory      complement(6193..6198)
                     /gene="agr_P2"
                     /regulatory_class="minus_10_signal"
     gene            complement(6193..6198)
                     /gene="agr_P2"
                     /label="agr_P2"
     regulatory      complement(6216..6221)
                     /gene="agr-P2"
                     /regulatory_class="minus_35_signal"
     gene            complement(6216..6221)
                     /gene="agr-P2"
                     /label="agr-P2"
     gene            6389..6417
                     /gene="agr-P3"
                     /label="agr-P3"
     regulatory      6389..6394
                     /gene="agr-P3"
                     /regulatory_class="minus_35_signal"
     regulatory      6412..6417
                     /gene="agr-P3"
                     /regulatory_class="minus_10_signal"
     CDS             6570..7274
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"