LOCUS       V005310                 7279 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005310
VERSION     V005310
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Scharschmidt TC, Rosenblum MD.
  TITLE     A wave of regulatory T cells into neonatal skin mediates tolerance
            to commensal microbes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Scharschmidt TC, Rosenblum MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2014) Dermatology, University of California San
            Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 7279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-OCT-2014) Dermatology, University of California San Francisco,
            513 Parnassus Ave, San Francisco, CA 94143, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..306
                     /regulatory_class="terminator"
     CDS             2837..3568
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        3936..4040
                     /label="AmpR promoter"
     CDS             4041..4898
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5072..5660
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5761..6165)
                     /codon_start=1
                     /product="ArgB"
                     /label="ArgB"
                     /protein_id="AKT95053.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLIV
                     MYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNFHI
                     NFLIMIILTVISLGVISVYAPSGRQLTQRR"
     regulatory      5792..6479
                     /gene="agr"
                     /regulatory_class="promoter"
     gene            5792..6479
                     /gene="agr"
                     /label="agr"
     regulatory      complement(6193..6198)
                     /gene="agr_P2"
                     /regulatory_class="minus_10_signal"
     gene            complement(6193..6198)
                     /gene="agr_P2"
                     /label="agr_P2"
     regulatory      complement(6216..6221)
                     /gene="agr-P2"
                     /regulatory_class="minus_35_signal"
     gene            complement(6216..6221)
                     /gene="agr-P2"
                     /label="agr-P2"
     gene            6389..6417
                     /gene="agr-P3"
                     /label="agr-P3"
     regulatory      6389..6394
                     /gene="agr-P3"
                     /regulatory_class="minus_35_signal"
     regulatory      6412..6417
                     /gene="agr-P3"
                     /regulatory_class="minus_10_signal"
     CDS             6570..7274
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
ORIGIN
        1 cgcgcctatt ctaatgcata ataaatactg ataacatctt atattttgta ttatattttg
       61 tattatcgtt gacatgtata attttgatat caaaaactga ttttccctct attattttcg
      121 agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca aaaaattata
      181 aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat
      241 ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa
      301 gtgacaggcg cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat
      361 ggtttagcag gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag
      421 caacaagaac aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat
      481 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      541 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      601 ctgtaggttc cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga
      661 aattcagaga agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag
      721 ttttaactgt ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga
      781 ccaaatcttt tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga
      841 ccaaagtttg tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg
      901 tatatttaga tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat
      961 tccttaaacg aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa
     1021 tcattcaaat catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa
     1081 tagccggttg gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat
     1141 gacgatcgtt ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt
     1201 agagccccaa ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca
     1261 agaaaaagtt tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat
     1321 gcgtattgaa tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa
     1381 tataataagc tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga
     1441 agatgatttg agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta
     1501 tggtcgtaat ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat
     1561 tagaatttat aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga
     1621 acatttatgg cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg
     1681 ctttagtgat ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag
     1741 agcaatagtt tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc
     1801 tagaacaaaa tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat
     1861 gaaatcgact ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca
     1921 tgaatttaaa ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt
     1981 aaactattct aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt
     2041 tatatgaggt gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta
     2101 aaattgttta gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt
     2161 tgtattcttt cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat
     2221 attgcaaatc attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg
     2281 ttaacttttt cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa
     2341 aaattgttaa ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg
     2401 cccacctagg aattgaatga gacatgctac acctccggat aataaatata tataaacgta
     2461 tatagatttc ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac
     2521 cgtgtgctct acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa
     2581 agaaattaga taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa
     2641 tttaacgatc actcatcatg ttcatattta tcagagctcg tgctataatt atactaattt
     2701 tataaggagg aaaaaatatg ggcattttta gtatttttgt aatcagcaca gttcattatc
     2761 aaccaaacaa aaaataagtg gttataatga atcgttaata agcaaaattc atataaccaa
     2821 attaaagagg gttataatga acgagaaaaa tataaaacac agtcaaaact ttattacttc
     2881 aaaacataat atagataaaa taatgacaaa tataagatta aatgaacatg ataatatctt
     2941 tgaaatcggc tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt
     3001 aactgccatt gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca
     3061 cgataatttc caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc
     3121 ctataaaata tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt
     3181 ttttgatagt atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt
     3241 attaaataca aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt
     3301 aagtatggtt ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag
     3361 attaagtaga aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt
     3421 tatgaaatgg gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc
     3481 cttaaaacat gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct
     3541 tttcaatagc tataaattat ttaataagta agttaaggga tgcataaact gcatccctta
     3601 acttgttttt cgtgtgccta ttttttgtga atcgattatg tcttttgcgc agtcggctta
     3661 aaccagtttt ccgcggcgct cgagcggccg catagttaag ccagccccga cacccgccaa
     3721 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3781 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3841 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3901 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5761 ttaccgcctt tgagtgagct ggcggccgct gggagcatat actgagatta cacctaaaga
     5821 aataactgtt aaaataatca taattaaaaa gttaatatga aaatttacta ttactaaagg
     5881 taaaagtata aatagtataa tactttctac ataacaccaa aaagaagaag gtgcatgtgc
     5941 accatgtgca tgtcttctta ttaaataaaa tgttaaattc gtaattaacg taaacagaaa
     6001 aatgtttaaa atataggcaa tagtatacat aacaattaat ttacctatat ttttagctaa
     6061 gacctgcatc cctaatcgta cttgcaaaaa ttgaatatga tctaagttat ttctcttttg
     6121 aagatacgtg gcaaactggt caattttatt atcaaaataa ttcaatttta caccactctc
     6181 ctcactgtca ttatacgatt tagtacaatc ttttatcatt atattgccta actgtaggaa
     6241 ataaatactt aactgttaaa tgtaatttgt atttaatatt ttaacataaa aaaatttaca
     6301 gttaagaata aaaaacgact agttaagaaa aattggaaaa taaatgcttt tagcatgttt
     6361 taatataact agatcacaga gatgtgatgg aaaatagttg atgagttgtt taattttaag
     6421 aatttttatc ttaattaagg aaggagtgat ttcaatggca caagatgggt accgagctcg
     6481 aattcttagg aggatgatta tttatggaag catggggagc tttagcaaat tgggcagttg
     6541 attcagctgg ttcaggttca ggttcaggtg tgagcaaggg cgaggaggat aatatggcta
     6601 ttatcaaaga atttatgaga ttcaaagttc atatggaagg ttcagtcaat ggtcacgaat
     6661 ttgaaattga gggagaaggt gaaggcagac catatgaagg tactcaaacg gccaaactta
     6721 aagtcactaa aggaggtcct cttccatttg catgggatat tcttagtcca caatttatgt
     6781 atggatctaa agcttacgtt aaacatccag cggacattcc ggattatctt aaattgtcgt
     6841 ttccagaagg cttcaaatgg gaaagagtaa tgaactttga agatggagga gtcgttacag
     6901 tcactcagga tagttctctt caagatggag aatttatcta caaagtcaag ttaagaggta
     6961 ccaattttcc ctcagatggt ccagttatgc aaaagaaaac tatgggatgg gaagcatcca
     7021 gtgagcgtat gtatcctgaa gatggtgccc ttaaaggcga aattaaacaa cgcctaaagc
     7081 ttaaagatgg tggacattat gacgctgaag ttaaaacaac ttacaaagcg aagaaaccgg
     7141 tacaacttcc aggtgcatat aatgtcaaca ttaaacttga tattacaagt cataatgaag
     7201 attatactat tgttgaacaa tatgagagag ctgaaggaag acattctaca ggcggcatgg
     7261 acgagctgta caagtaagg
//
