pJK95 vector (V005320)

LOCUS       V005320                 4791 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005320
VERSION     V005320
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Blodgett JA, Zhang JK, Metcalf WW.
  TITLE     Molecular cloning, sequence analysis, and heterologous expression of
            the phosphinothricin tripeptide biosynthetic gene cluster from
            Streptomyces viridochromogenes DSM 40736
  JOURNAL   Antimicrob. Agents Chemother. 49 (1), 230-240 (2005)
   PUBMED   15616300
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Blodgett JAV., Zhang JK, Metcalf WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2004) Microbiology, University of Illinois, 601 S.
            Goodwin, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "2005"; volume: "49"; issue: "1"; pages:
            "230-240"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2004) Microbiology, University of Illinois, 601 S. Goodwin,
            Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   20..40
                     /label="derived from bacteriophage Mu"
                     /note="derived from bacteriophage Mu"
     CDS             complement(104..910)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     rep_origin      1114..1635
                     /label="oriV"
                     /note="incP origin of replication"
     promoter        1746..2075
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2436..3227
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     mobile_element  3290..3310
                     /mobile_element_type="transposon:inverted"
                     /label="ted"
                     /note="derived from bacteriophage Mu"
     rep_origin      complement(3351..3739)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3804..4661)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4662..4766)
                     /label="AmpR promoter"