LOCUS       V005320                 4791 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005320
VERSION     V005320
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Blodgett JA, Zhang JK, Metcalf WW.
  TITLE     Molecular cloning, sequence analysis, and heterologous expression of
            the phosphinothricin tripeptide biosynthetic gene cluster from
            Streptomyces viridochromogenes DSM 40736
  JOURNAL   Antimicrob. Agents Chemother. 49 (1), 230-240 (2005)
   PUBMED   15616300
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Blodgett JAV., Zhang JK, Metcalf WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2004) Microbiology, University of Illinois, 601 S.
            Goodwin, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 4791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "2005"; volume: "49"; issue: "1"; pages:
            "230-240"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2004) Microbiology, University of Illinois, 601 S. Goodwin,
            Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   20..40
                     /label="derived from bacteriophage Mu"
                     /note="derived from bacteriophage Mu"
     CDS             complement(104..910)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     rep_origin      1114..1635
                     /label="oriV"
                     /note="incP origin of replication"
     promoter        1746..2075
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2436..3227
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     mobile_element  3290..3310
                     /mobile_element_type="transposon:inverted"
                     /label="ted"
                     /note="derived from bacteriophage Mu"
     rep_origin      complement(3351..3739)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(3804..4661)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4662..4766)
                     /label="AmpR promoter"
ORIGIN
        1 ggcgcgccga attcagatct gaagcggcgc acgaaaaacg cgaaagcgtt tcacgataaa
       61 tgcgaaaacg gatcgcggcc gctccgagga acagaggcgc ttatcggttg gccgcgagat
      121 tcctgtcgat cctctcgtgc agcgcgattc cgagggaaac ggaaacgttg agagactcgg
      181 tctggctcat catggggatg gaaaccgagg cggaagacgc ctcctcgaac aggtcggaag
      241 gcccaccctt ttcgctgccg aacagcaagg ccagccgatc cggattgtcc ccgagttcct
      301 tcacggaaat gtcgccatcc gccttgagcg tcatcagctg cataccgctg tcccgaatga
      361 aggcgatggc ctcctcgcga ccggagagaa cgacgggaag ggagaagacg taacctcggc
      421 tggccctttg gagacgccgg tccgcgatgc tggtgatgtc actgtcgacc aggatgatcc
      481 ccgacgctcc gagcgcgagc gacgtgcgta ctatcgcgcc gatgttcccg acgatcttca
      541 ccccgtcgag aacgacgacg tccccacgcc ggctcgcgat atcgccgaac ctggccgggc
      601 gagggacgcg ggcgatgccg aatgtcttgg ccttccgctc ccccttgaac aactggttga
      661 cgatcgagga gtcgatgagg cggaccggta tgttctgccg cccgcacaga tccagcaact
      721 cagatggaaa aggactgctg tcgctgccgt agacctcgat gaactccacc ccggccgcga
      781 tgctgtgcat gaggggctcg acgtcctcga tcaacgttgt ctttatgttg gatcgcgacg
      841 gcttggtgac atcgatgatc cgctgcaccg cgggatcgga cggatttgcg atggtgtcca
      901 actcagtcat ggtcgtccta ccggctgctg tgttcagtga cgcgattcct ggggtgtgac
      961 accctacgcg acgatggcgg atggctgccc tgaccggcaa tcaccaacgc aaggggaagt
     1021 cgtcgctctc tggcaaagct ccccgctctt ccccgtccgg gacccgcgcg gtcgatcccc
     1081 gcatatgaag tattcgcctt gatcaccgtc tagaccggcg ttgtggatac ctcgcggaaa
     1141 acttggccct cactgacagc tgaggggcgg acgttgacac ttgaggggcc gactcacccg
     1201 gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag
     1261 cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa
     1321 gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag
     1381 gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga
     1441 catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt
     1501 tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt
     1561 ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     1621 ctcgaaccct cccggtggcc ggccgcggat ccttggcaga acatatccat cgcgtccgcc
     1681 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctgcag atccggctgt
     1741 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1801 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     1921 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2041 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tcacgctgcc gcaagcactc
     2101 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
     2161 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
     2221 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
     2281 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
     2341 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
     2401 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     2461 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2521 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2581 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     2641 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2701 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2761 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2821 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2881 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2941 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3001 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3061 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3121 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3181 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3241 ggggttcgaa atgaggatcc gttttcgcat ttatcgtgaa acgctttcgc gtttttcgtg
     3301 cgccgcttca gatctgaatt cggcgcgcct ctagctagag gatctaattc tgtcagccgt
     3361 taagtgttcc tgtgtcactg aaaattgctt tgagaggctc taagggcttc tcagtgcgtt
     3421 acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat
     3481 tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta
     3541 attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc
     3601 atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg
     3661 ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg
     3721 aactgctgat cttcagatcc tctacgccgg acgcatcgtg gggatctaaa aaaaagcccg
     3781 ctcattaggc gggctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
