pJK100 vector (V005321)

LOCUS       40924_26166        3854 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic exchange vector pJK100, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JL, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Genetic and Genomic Insights into the Role of Benzoate-Catabolic 
            Pathway Redundancy in Burkholderia xenovorans LB400
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 585-595 (2006)
  PUBMED    16391095
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JM, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2005) Center for Microbial Ecology, Michigan State
REFERENCE   3  (bases 1 to 3854)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "1"; pages:
            "585-595"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2005) Center for Microbial Ecology, Michigan State 
            University, 540 Plant "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     pJK100 was derived from the 1674 bp BsgI-AatII fragment of pCM184 
            (Marx
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(881..1269)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            1440..1549
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             1749..2936
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     protein_bind    complement(3108..3141)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3275..3854,1..233)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"