LOCUS       40924_26166        3854 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic exchange vector pJK100, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JL, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Genetic and Genomic Insights into the Role of Benzoate-Catabolic 
            Pathway Redundancy in Burkholderia xenovorans LB400
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 585-595 (2006)
  PUBMED    16391095
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Denef VJ, Klappenbach JA, Patrauchan MA, Florizone C, Rodrigues JM, 
            Tsoi TV, Verstraete W, Eltis LD, Tiedje JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2005) Center for Microbial Ecology, Michigan State
REFERENCE   3  (bases 1 to 3854)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "1"; pages:
            "585-595"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2005) Center for Microbial Ecology, Michigan State 
            University, 540 Plant "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     pJK100 was derived from the 1674 bp BsgI-AatII fragment of pCM184 
            (Marx
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(881..1269)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            1440..1549
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             1749..2936
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     protein_bind    complement(3108..3141)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3275..3854,1..233)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
       61 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
      121 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
      181 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
      241 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct
      301 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
      361 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
      421 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
      481 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgcccccccc cccctgcagg
      541 tcctagataa cttcgtatag catacattat acgaagttat ggatccagct tatcgatacc
      601 gcgggcccta cgtacgcgtg ttaaccggtg agctcactag aggatccagc cgaccaggct
      661 ttccacgccc gcgtgccgct ccatgtcgtt cgcgcggttc tcggaaacgc gctgccgcgt
      721 ttcgtgattg tcacgctcaa gcccgtagtc ccgttcgagc gtcgcgcaga ggtcagcgag
      781 ggcgcggtag gcccgatacg gctcatggat ggtgtttcgg gtcgggtgaa tcttgttgat
      841 ggcgatatgg atgtgcaggt tgtcggtgtc gtaattccca tgtcagccgt taagtgttcc
      901 tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt acatccctgg
      961 cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat tctttttttt
     1021 cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta attttattgt
     1081 tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc atgagagctt
     1141 agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg ttaaacatga
     1201 gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg aactgctgat
     1261 cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgttttc cgctgcataa
     1321 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     1381 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     1441 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     1501 tcgcacctgg cggtgctcaa cgggaatcct gctctgcnag gctggccggc taccgccggc
     1561 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     1621 cctatcacgg aattgatccc cctcgaattg acgcgtcaat tctcatgttt gacagcttat
     1681 catcgataag ctagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
     1741 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
     1801 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt
     1861 ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc
     1921 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
     1981 cttcgctact tggagccact atcgattacg cgatcatggc gaccacaccc gtcctgtgga
     2041 ttctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     2101 cttatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     2161 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     2221 ccttacatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     2281 gcttcctaat gcaggagtcg cataagggag agcgccgacc catgcccttg agagccttca
     2341 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     2401 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     2461 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     2521 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     2581 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     2641 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     2701 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     2761 gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga
     2821 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
     2881 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
     2941 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     3001 cttgcggaga actgtgaatg cgcaaaccaa cccttgatca agctgacgcg tcctctagat
     3061 ctgaattcag ctgtacaatt ggtaccatgg atgcatatgg cggccgcata acttcgtata
     3121 gcatacatta tacgaagtta tctagacctg cagggggggg ggggaaagcc acgttgtgtc
     3181 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     3241 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     3301 ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     3361 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     3421 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3481 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3541 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
     3601 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3661 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3721 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3781 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     3841 accggattca gtcg
//
