pJG032 vector (V005342)

LOCUS       V005342                 9402 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005342
VERSION     V005342
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 9402)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..780
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(1030..1618)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1792..2649)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2650..2754)
                     /label="AmpR promoter"
     misc_feature    2974..3873
                     /label="amyE front"
                     /note="amyE front"
     CDS             complement(3881..4933)
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90152.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            complement(3881..4933)
                     /gene="xylR"
                     /label="xylR"
     regulatory      5160..5167
                     /label="-35 (sigA)"
                     /note="-35 (sigA)"
                     /regulatory_class="minus_35_signal"
     regulatory      5185..5190
                     /label="-10 (sigA)"
                     /note="-10 (sigA)"
                     /regulatory_class="minus_10_signal"
     protein_bind    5200..5210
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     protein_bind    5214..5224
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     CDS             5294..5353
                     /codon_start=1
                     /gene="xylA"
                     /product="XylA fragment"
                     /label="xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90153.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            5294..5353
                     /gene="xylA"
                     /label="xylA"
     CDS             5382..5912
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /protein_id="APU90154.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            5382..5912
                     /gene="loaP"
                     /label="loaP"
     CDS             6273..6920
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    7294..8294
                     /label="amyE back"
                     /note="amyE back"