LOCUS       V005342                 9402 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005342
VERSION     V005342
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 9402)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..780
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(1030..1618)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1792..2649)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2650..2754)
                     /label="AmpR promoter"
     misc_feature    2974..3873
                     /label="amyE front"
                     /note="amyE front"
     CDS             complement(3881..4933)
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90152.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            complement(3881..4933)
                     /gene="xylR"
                     /label="xylR"
     regulatory      5160..5167
                     /label="-35 (sigA)"
                     /note="-35 (sigA)"
                     /regulatory_class="minus_35_signal"
     regulatory      5185..5190
                     /label="-10 (sigA)"
                     /note="-10 (sigA)"
                     /regulatory_class="minus_10_signal"
     protein_bind    5200..5210
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     protein_bind    5214..5224
                     /label="XylR binding site"
                     /bound_moiety="XylR"
                     /note="xylO operator"
     CDS             5294..5353
                     /codon_start=1
                     /gene="xylA"
                     /product="XylA fragment"
                     /label="xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90153.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            5294..5353
                     /gene="xylA"
                     /label="xylA"
     CDS             5382..5912
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /protein_id="APU90154.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            5382..5912
                     /gene="loaP"
                     /label="loaP"
     CDS             6273..6920
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    7294..8294
                     /label="amyE back"
                     /note="amyE back"
ORIGIN
        1 atgagcaatt tgattaacgg aaaaatacca aatcaagcga ttcaaacatt aaaaatcgta
       61 aaagatttat ttggaagttc aatagttgga gtatatctat ttggttcagc agtaaatggt
      121 ggtttacgca ttaacagcga tgtagatgtt ctagtcgtcg tgaatcatag tttacctcaa
      181 ttaactcgaa aaaaactaac agaaagacta atgactatat caggaaagat tggaaatacg
      241 gattctgtta gaccacttga agttacggtt ataaatagga gtgaagttgt cccttggcaa
      301 tatcctccaa aaagagaatt tatatacggt gagtggctca ggggtgaatt tgagaatgga
      361 caaattcagg aaccaagcta tgatcctgat ttggctattg ttttagcaca agcaagaaag
      421 aatagtattt ctctatttgg tcctgattct tcaagtatac ttgtctccgt acctttgaca
      481 gatattcgaa gagcaattaa ggattctttg ccagaactaa ttgaggggat aaaaggtgat
      541 gagcgtaatg taattttaac cctagctcga atgtggcaaa cagtgactac tggtgaaatt
      601 acctcgaaag atgtcgctgc agaatgggct atacctcttt tacctaaaga gcatgtaact
      661 ttactggata tagctagaaa aggctatcgg ggagagtgtg atgataagtg ggaaggacta
      721 tattcaaagg tgaaagcact cgttaagtat atgaaaaatt ctatagaaac ttctctcaat
      781 taggctaatt ttattgcaat aacaggtgct tacttttctg gagttcttta gcaaattttt
      841 ttattagctg aacttagtat tagtggccat actcctccaa tccaaagcta tttagaaaga
      901 ttactatatc ctcaaacagg cggtaaccgg cctcttcatc gggaatgcgc gcgaccttca
      961 gcatcgccgg catgtccccc tggcggacgg gaagtatcca gctcgaggtc gggccgcgtt
     1021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1441 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1501 gccagttacc ttcggaaaaa gagttgatag ctcttgatcc ggcaaacaaa ccaccgctgg
     1561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2101 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2401 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2761 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2821 aaataggcgt atcacgaggc cctttcgtct tcaagaatta acaaaattct ccagtcttca
     2881 catcggtttg aaaggaggaa gcggaagaat gaagtaagag ggatttttga ctccgaagta
     2941 agtcttcaaa aaatcaaata aggagtgtca agaatgtttg aaaaacgatt caaaacctct
     3001 ttactgccgt tattcgccgg atttttattg ctgtttcatt tggttttgtc aggcccggcg
     3061 gctgcaaacg ctgaaactgc aaacaaatcg aataaggtga ccgcgtcatc ggtcaaaaac
     3121 gggaccatcc ttcatgcatg gaattggtcg ttcaatacgt taacacaaaa tatgaaagat
     3181 attcgtgatg cgggctatgc agccattcag acgtctccga ttaaccaagt aaaggaaggg
     3241 aaccaaggag ataaaagcat gaggaactgg tactggctgt atcagccgac atcgtaccaa
     3301 atcggcaacc gttacttagg cactgaacaa gaatttaagg acatgtgtgc agcggcggaa
     3361 aaatatggcg taaaagtcat tgtcgatgcg gttatcaatc atactaccag cgattatggc
     3421 gcgatttctg acgagattaa gcgtattcca aactggaccc atggaaacac acaaattaaa
     3481 aattggtcgg atcgatggga cgtcactcaa aattcattgc tcgggctgta tgattggaat
     3541 acacagaata ctgaggtgca ggtctatctg aaacgtttct tggaaagagc attgaatgac
     3601 ggagcagacg ggttccgcta tgatgccgcc aagcatatag agcttccgga tgacgggaat
     3661 tacggcagcc aattttggcc gaatatcaca aatacatcag cggagttcca atacggagaa
     3721 atcctgcaag acagcgcgtc cagagatact gcttatgcga attatatgaa tgtgacggct
     3781 tctaactacg ggcattccat cagatccgct ttaaagaacc gtaatctgag tgtgtcgaat
     3841 atctcccatt atgcatctga cgtgtctgcg gacgaagctt ttacatttta acgatatcta
     3901 gaaaatgttc aataacaatg gaagacattc ctaatgccgg tgcattcttt cctaaggaag
     3961 atggtaataa ttcatagcta ttgcctaatt ggggataaac cctggacgac acttcacttc
     4021 taattgaatt taaaaccata ggatgtgatt caattatgct gtttcttaaa atgatggctt
     4081 gtggattgaa tgtatttaga atattagtaa ggccaattcc taaatagaat ccgaaattct
     4141 gtaatgcatt taaggtgccg atatcattca gatgggcgag gtctatgata tcttgatagg
     4201 acactttttt ctctttagtc tgaagagatt ttaataaagc cttctctgaa gcatacaatt
     4261 cccagcaacc tcggtttccg caactgcatt taggaccatt aaagtctatt gtcatatgtc
     4321 ccatttctcc agagaatccg cttacccctc tatataaatg attgttgata ataacaccga
     4381 tccctatacc tgtactgata ctagcataaa taatgttatt gtgatttttt gcagcaccaa
     4441 atactttttc tccatacgcg ccagcatttg cctcattttc aataaaaaca ggcacattga
     4501 acttctcttg tatgaaagat tttaagtcaa tatctctcca gttggagttc ggagtgaaaa
     4561 caattttttg atttttatca attagtccag gcacgcatat gcctataccg ataagcccgt
     4621 acggagattg tggcatacgc gtaataaagt gatgaatcat atcaattaaa atgtctttag
     4681 ttatttctgg agaattcgat tctaaatggt gatgttgatc aagaatgatc gttccttcaa
     4741 ggtctgttaa aatgccacta atataatcca caccaacatc tattcctatg gagtatcctg
     4801 cctttttatt aaaaacaagc atgacaggtc ttcttccgcc acttgattgt ccttgaccta
     4861 tttcaaatac aagattttct ttcatcagcg tgtttacctg tgatgaaaca gttgatttat
     4921 ttaatccagt catttcagat aattttgctc ttgaaatagg tgaattctta agtatttctt
     4981 ttaataataa cttttgattt acttttttga caaaggtttg atctgcgata tccacttcat
     5041 ccactccatt tgtataacct ttaaattaag ttaaaatttt ttgtgttcag tatgagattt
     5101 agtacatagc gaatcttacc tttattatat ctaatgtgtt catgaaaaac taaaaaaaat
     5161 attgaaaata ctgatgaggt tatttaagat taaaataagt tagtttgttt gggcaacaaa
     5221 ctaatgtgca acttacttac aatatgacat aaaatgcatc tgtatttgaa tttattttta
     5281 aggaggaaat aacatggctc aatctcattc tagttcagtt aactattttg taagcgttaa
     5341 caaagtggtt taaattaagt cgacagaagg aggacccagt catgaaatgg tacgcacttt
     5401 ttgtagagtc cggaaaagaa gaaacagttc aaaaatttct gcgtctgcag tttgatgagc
     5461 aggctctcta ctcaatcata cccaagaaaa aagtcacaga acgaaaagcc ggaatcaaat
     5521 acgaggcact caaaaagatg tttccgggat atgtgctgtt taaaacgaag atgacagagc
     5581 ggacatttca taaaatcaaa gaactcccca tttcatgcag aatcgtgaat aacggcgcat
     5641 attattccaa agagcgtaaa acctatttca cgacaattaa ggatgaagaa atcctcccta
     5701 ttatcaggct gatcggagaa ggagacactg tagattattc aaaagtctat attgaaaatt
     5761 caaaagtaac cgttgcctca gggcctctta aaggaatgga aggcattatt aaaaagatcg
     5821 ataaaaggaa aagacgcgcc aaaatctgcc tgtcttttat gggactggac aaaatggtca
     5881 acgtcggaat tgaagtgctc tccaaaccct agcatatcaa aaaaagcatc aacagcatgt
     5941 gccgttgatg ctttttttgg atccctcatg tttgacagct tatcatcggc aatagttacc
     6001 cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac
     6061 acaaaagacc acatttttta atgtggtctt tattcttcaa ctaaagcacc cattagttca
     6121 acaaacgaaa attggataaa gtgggatatt tttaaaatat atatttatgt tacagtaata
     6181 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
     6241 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
     6301 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
     6361 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
     6421 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
     6481 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
     6541 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
     6601 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
     6661 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
     6721 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
     6781 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
     6841 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
     6901 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
     6961 ttctaatgtc actaacctgc cccgttagtt gaagaaggtt tttatattac agctccagat
     7021 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     7081 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     7141 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     7201 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     7261 cttcctaatg caggagtcgc ataagggaga gcgttcgttt aggctgggca gtgattggtt
     7321 cccgctcagg aagcacgcct cttttctttt ccagacctga gggcggagga aatggtgtaa
     7381 gatttcccgg aaaaagtcaa ataggagatc gcgggagcgc cttatttaaa gatcaggcga
     7441 tcactgcggt caatacattt cacaatgtaa tggccgggca gcccgaggaa ctctcgaatc
     7501 cgaatgggaa caaccaagtc tttatgaatc agcgcggctc aaaaggcgtt gtgctggcaa
     7561 atgcgggatc gtcttctgtc accatcaata cttcagcgaa attacctgac ggcaggtatg
     7621 ataatagggc cggcgccggt tcatttcaag tagcgaacgg caaactgaca ggtacgatca
     7681 atgccagatc cgcggctgtt ctttatcctg atgatattgg aaatgcgcct catgtctttc
     7741 ttgagaatta ccaaacgggg gcagtccatt ctttcaatga tcagctgacg gtcaccctgc
     7801 gtgcaaatgc gaaaacaaca aaagccgttt accaaatcaa taatgggcag cagacagcat
     7861 ttaaggatgg agaccgacta acgatcggga aaggagatcc aatcggcacg acatacaaca
     7921 tcaaattaac cggaacgaac ggcgagggtg cagcgagaac ccaagaatac acatttgtca
     7981 aaaaagaccc gtcccaaacc aacatcattg gctatcaaaa tccggaccat tggggccagg
     8041 taaatgctta tatttacaaa catgatggag gcagggccat agaattaacc ggatcgtggc
     8101 cggggaaagc aatgactaag aatgcaaatg gaatgtacac gctgacgctg cctgagaata
     8161 cggatacggc caatgccaaa gtgattttta acaatggcag cgcccaagtg cccggacaga
     8221 accagcccgg ctttgattat gtgcagaatg gtttgtataa caactccggt ttgaatggtt
     8281 atcttccgca ttaagggcaa ggctagacgg gacttaccga aagaaaccat caatgatggt
     8341 ttcttttttg ttcataaatc agacaaaact tttctcttgc aaaagtttgt gaagtgttgc
     8401 acaatataaa tgtgaaatac ttcacaaaca aaaagacatc aaagagaaac ataccctgca
     8461 aggatgctga tattgtctgc atttgcgccg gagcaaacca aaaacctggt gagacacgcc
     8521 ttgaattagt agaaaagaac ttgaagattt tcaaaggcat cgttagtgaa gtcatggcga
     8581 gcggatttga cggcattttc ttagtcgcga cgcgaggctg gatggccttc cccattatga
     8641 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     8701 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     8761 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     8821 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     8881 gtgcatggag ccgggccacc tactgaagtg gatttcttta agagctcctt taacttcctc
     8941 accagtagtt gtatcggtac cataagtaga agcagcaacc caagtagctt taccagcatc
     9001 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc
     9061 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct
     9121 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc
     9181 cgggcccgcg gatgcatatg atcagatctt aaggcctagg tctagaggat cgatctgtat
     9241 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
     9301 gtcagcttta ttgaacagta atttaagtat atgtccaatc tagggtaagt aaattgagta
     9361 tcaatataaa ctttatatga acataatcaa cgaggtgaaa tc
//
