pJG030 vector (V005343)

LOCUS       V005343                 5607 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005343
VERSION     V005343
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    397..1294
                     /note="LoaP region homology; homology region upstream of
                     loaP containing dfnA leader"
     gene            complement(397..528)
                     /pseudo=""
                     /gene="dfnA"
                     /label="dfnA"
     gene            1262..1294
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="5'"
     CDS             1745..2476
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2573..3342
                     /note="LoaP Region Homology; homology region downstream of
                     loaP"
     gene            2573..2603
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="3'"
     gene            complement(2947..3342)
                     /pseudo=""
                     /locus_tag="RBA_022100"
                     /label="RBA_022100"
     misc_feature    3343..3373
                     /note="MCS; pUC19 multiple cloning site"
     primer_bind     complement(3386..3402)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3410..3426)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3434..3464)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3479..3500)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3788..4376)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4550..5407)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5408..5512)
                     /label="AmpR promoter"