LOCUS       V005343                 5607 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005343
VERSION     V005343
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    397..1294
                     /note="LoaP region homology; homology region upstream of
                     loaP containing dfnA leader"
     gene            complement(397..528)
                     /pseudo=""
                     /gene="dfnA"
                     /label="dfnA"
     gene            1262..1294
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="5'"
     CDS             1745..2476
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2573..3342
                     /note="LoaP Region Homology; homology region downstream of
                     loaP"
     gene            2573..2603
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="3'"
     gene            complement(2947..3342)
                     /pseudo=""
                     /locus_tag="RBA_022100"
                     /label="RBA_022100"
     misc_feature    3343..3373
                     /note="MCS; pUC19 multiple cloning site"
     primer_bind     complement(3386..3402)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3410..3426)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3434..3464)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3479..3500)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3788..4376)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4550..5407)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5408..5512)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaga cacaattctt ctatagagta
      421 gcctaatatg tcatcagcct gacgtgtcag atcttgaaat tcatcaaaca gtccggctcc
      481 cattccttta aattgagaac cctgccccgg gaacacaaac gtaagcatct tatcctcatc
      541 ctttccgcat cgccaatttt gtatatcaca tgtggatctc catacaaaaa cgtctcttat
      601 ttcatatatt gactggtgta aacaccggca taaaaataaa aacacaaacg cgtttgcgat
      661 tcagcacagg tttaaaaatc gcaaacgcat tgtgttttcc ttttctaaca taatttatat
      721 aaagacatct tgcttgttta ctgaatgttt cgcatgacaa gttgcagtgc caatatgccc
      781 gaaaacagcc atgacggcac acttctccct tatcatgtat atgtagatat atacactaca
      841 atcattttta taatccctat ttcctcttca acgtgccgaa acagcgattg aaatttaata
      901 ggtgctttac atcaatttaa taaatttaac aaatagttgc tatttaaaga gaataatatc
      961 aatttataga taataatgtc aaggttttat ttcgttattt tctgacattt attccacagt
     1021 tttatgttcg gtggacagtc ggcataattg tttcgggata aatgtcagat aattccctga
     1081 accctattca tgtcaaagaa aattcatcgc ttctctgtca attgggtcta tacttatttt
     1141 tcattttcat atattatgta aaaaaaagac gaattagaaa aatttttaat cactttaaat
     1201 actatccatt aaatactttc tttgtataat aatttcttat tattctaagg aggacccagt
     1261 catgaaatgg tacgcacttt ttgtagagtc cggatccttt aactctggca accctcaaaa
     1321 ttgaatgaga catgctacac ctccggataa taaatatata taaacgtata tagatttcat
     1381 aaagtctaac acactagact tatttacttc gtaattaagt cgttaaaccg tgtgctctac
     1441 gaccaaaact ataaaacctt taagaacttt ctttttttac aagaaaaaag aaattagata
     1501 aatctctcat atcttttatt caataatcgc atccgattgc agtataaatt taacgatcac
     1561 tcatcatgtt catatttatc agagctcgtg ctataattat actaatttta taaggaggaa
     1621 aaaatatggg catttttagt atttttgtaa tcagcacagt tcattatcaa ccaaacaaaa
     1681 aataagtggt tataatgaat cgttaataag caaaattcat ataaccaaat taaagagggt
     1741 tataatgaac gagaaaaata taaaacacag tcaaaacttt attacttcaa aacataatat
     1801 agataaaata atgacaaata taagattaaa tgaacatgat aatatctttg aaatcggctc
     1861 aggaaaaggc cattttaccc ttgaattagt aaagaggtgt aatttcgtaa ctgccattga
     1921 aatagaccat aaattatgca aaactacaga aaataaactt gttgatcacg ataatttcca
     1981 agttttaaac aaggatatat tgcagtttaa atttcctaaa aaccaatcct ataaaatata
     2041 tggtaatata ccttataaca taagtacgga tataatacgc aaaattgttt ttgatagtat
     2101 agctaatgag atttatttaa tcgtggaata cgggtttgct aaaagattat taaatacaaa
     2161 acgctcattg gcattacttt taatggcaga agttgatatt tctatattaa gtatggttcc
     2221 aagagaatat tttcatccta aacctaaagt gaatagctca cttatcagat taagtagaaa
     2281 aaaatcaaga atatcacaca aagataaaca aaagtataat tatttcgtta tgaaatgggt
     2341 taacaaagaa tacaagaaaa tatttacaaa aaatcaattt aacaattcct taaaacatgc
     2401 aggaattgac gatttaaaca atattagctt tgaacaattc ttatctcttt tcaatagcta
     2461 taaattattt aataagtaag ttaagggatg cataaactgc atcccttaac ttgtttttcg
     2521 tgtgcctatt ttttgtgaat cgatgataag ctgtcaaaca tgagaattaa ttcgtcggaa
     2581 ttgaagtgct ctccaaaccc tagcatatca aaaaaagcat caacagcatg tgccgttgat
     2641 gcttttttta tataaacaga cgaaagcccg acaccaaaat aaccccgctc accaccgtca
     2701 cggtcagact ttttgttttc aaggcatagt tgacctgaag ccgggctgcg tccatgaagc
     2761 aaaatcacat ttaggggaag atacattttt atcaaaagcg gtgaattgac ggttgagctt
     2821 catgacgaaa agcatgtgct gacttcgggc gatgccgcac agttatttta attccacgga
     2881 tgaaccggtg acatatttta tgctggtcta ttatcctgaa ccggcttaaa cgcacatcag
     2941 gcttcttcag acagaagcct gatggaccgc aacccattct tgtatgcatt gaatcagttt
     3001 ctggacagaa ggtgtctgcg tctcctttgc atgagctgtc aaaatgccga gctgcctgga
     3061 gacataccgg tcgaaccgtt tgatgagcac atcacaatca ttcaccaaaa gctgcggcaa
     3121 gatgctgatc cccagattgt tccgcaccat tgcaataatc gcttcatccc ctctgatttg
     3181 ataagcgacc cgcggcgtaa tcttttcttc cttcagcact ttcagcacat caaaatgtgt
     3241 ctccggataa ggcacgatac atttgaccga ctccatgaca tgtaacggaa ccgtttcaca
     3301 ttgcgccaat tcatgctgcg ggctcatcac cgctacaatc ggctctagag tcgacctgca
     3361 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3481 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4561 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4621 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4681 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4741 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4801 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4861 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4921 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4981 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5041 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5101 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5161 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5221 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5281 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5341 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5401 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5461 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5521 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     5581 ataggcgtat cacgaggccc tttcgtc
//
