pJFR4 vector (V005346)

LOCUS       40924_26036        6267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 6267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      253..275
                     /label=Promoter T3 (T7 RNAP)
                     /note="Promoter T3 (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             311..1000
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     misc_feature    1022..1171
                     /label=Terminator DT5
                     /note="Terminator DT5"
     regulatory      1212..1234
                     /label=Promoter K1F (T7 RNAP)
                     /note="Promoter K1F (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             1264..1938
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     CDS             1945..1968
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1982..2140
                     /label=Terminator DT16
                     /note="Terminator DT16"
     terminator      complement(2176..2223)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2247..2960)
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     regulatory      2996..3018
                     /label=Promoter CCG (T7 RNAP)
                     /note="Promoter CCG (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             complement(3573..4520)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(4568..4790)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5282..5376)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5410..6267)
                     /label=AmpR
                     /note="beta-lactamase"