LOCUS       40924_26036        6267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 6267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      253..275
                     /label=Promoter T3 (T7 RNAP)
                     /note="Promoter T3 (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             311..1000
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     misc_feature    1022..1171
                     /label=Terminator DT5
                     /note="Terminator DT5"
     regulatory      1212..1234
                     /label=Promoter K1F (T7 RNAP)
                     /note="Promoter K1F (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             1264..1938
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     CDS             1945..1968
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1982..2140
                     /label=Terminator DT16
                     /note="Terminator DT16"
     terminator      complement(2176..2223)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2247..2960)
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     regulatory      2996..3018
                     /label=Promoter CCG (T7 RNAP)
                     /note="Promoter CCG (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             complement(3573..4520)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(4568..4790)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5282..5376)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5410..6267)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcgcgaa acgatcctca tcctgtctct
       61 tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
      121 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cgacgtctaa
      181 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      241 cttcacctcg agtaataacc ctcactatag ggagaggatc ctgttaattt ccgcgataga
      301 ggaggtataa atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
      361 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cttacgaagg
      421 cacccagacc atgcgtatca aggtagtaga agggggtcca ctgccgttcg ccttcgatat
      481 ccttgctact tcttttctgt acggttccaa aaccttcatc aaccacacac agggtatccc
      541 ggacttcttc aagcagagct tcccagaagg ttttacctgg gaacgcgtca ctacctacga
      601 agacggtggt gtactgaccg ccactcagga cacttcgctg caggacggtt gcctgatcta
      661 caacgtcaaa attcgtggtg tcaacttcac aagcaacggt ccagtgatgc agaaaaaaac
      721 cctgggttgg gaagcgttca cagaaaccct gtatccagcg gacggtggtt tggaaggccg
      781 caacgacatg gcactgaaac tggtcggtgg ctctcacctt atcgctaaca tcaaaactac
      841 gtaccgttct aaaaaaccag ccaaaaacct caagatgccg ggtgtgtact atgtcgatta
      901 ccgtctggaa cgtatcaaag aagccaacaa cgaaacttac gttgaacagc acgaagtggc
      961 cgtggcgcgt tactgtgatt tgccgagcaa actgggtcat taataaggat ctccggcaat
     1021 taaaaaagcg gctaaccacg ccgctttttt tacgtctgca tccttggccc tccatcctta
     1081 gatagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
     1141 tggtcctcat aggcaatacg atcgcatgtc cctgttgttt gtcggtgaac gctctcctga
     1201 gtaggacaaa ttaataacta tcactatagg gagaggatcc tatcaatttc cccgatacag
     1261 gagttaacga tgaaagaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1321 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1381 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1441 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1501 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1561 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1621 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1681 accatgggtt gggaagcttc caccgaacgt atgtacccag aagacggtgc tctgaaaggt
     1741 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1801 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1861 gacatcacct cccacaacaa agactacacc atcgttgaac agtacgaacg tgctgaaggt
     1921 cgtcactcca ccggtgctgg tggcgattat aaagatgatg atgataaata ataagagctc
     1981 gctcggtacc aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt
     2041 cctccttggc cctccatcct tagatgtccg gcaattaaaa aagcggctaa ccacgccgct
     2101 ttttttacgt ctgcatcata ggcaatacga tcgcatgtcc ccgccgccct agacctaggg
     2161 tccgacaaac aacagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     2221 taggatcctt attaagcacc ttattatttg tagagctcat ccatgccatg tgtaatccca
     2281 gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa
     2341 aggacagatt gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
     2401 ggagtatttt gttgataatg gtctgctagt tgaacggaac catcttcaac gttgtggcga
     2461 attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta tacattgtgt
     2521 gagttaaagt tgtactcgag tttgtgtcca agaatgtttc catcttcttt aaaatcaata
     2581 ccctttaact cgatacgatt aacaagggta tcaccttcaa acttgacttc agcacgcgtc
     2641 ttgtaggtcc cgtcatcttt gaaagatata gtgcgttcct gtacataacc ttcgggcatg
     2701 gcactcttga aaaagtcatg ccgtttcatg tgatccggat aacgggaaaa gcattgaaca
     2761 ccataggtca gagtagtgac aagtgttggc cacggaacag gtagttttcc agtagtgcaa
     2821 ataaatttaa gggtgagttt tccgtttgta gcatcacctt caccctctcc acggacagaa
     2881 aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa
     2941 agttcttctc ctttgctcat ctttacctcc tctatcgcgg aaattgacag gatcctctcc
     3001 ctatagtgac cggtattact cgaggtgaag acgaaagggc ctcgtgatac gccacgggtt
     3061 ttgctgcccg caaacgggct gttctggtgt tgctagtttg ttatcagaat cgcagatccg
     3121 gcttcagccg gtttgccggc tgaaagcact atttcttcca gaattgccat gattttttcc
     3181 ccacgggagg cgtcactggc tcccgtgttg tcggcagctt tgattcgata agcagcatcg
     3241 cctgtttcag gctgtctatg tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa
     3301 tttcatgttc tagttgcttt gttttactgg tttcacctgt tctattaggt gttacatgct
     3361 gttcatctgt tacattgtcg atctgttcat ggtgaacagc tttaaatgca ccaaaaactc
     3421 gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat atggacagtt
     3481 ttccctttga tatctaacgg tgaacagttg ttctactttt gtttgttagt cttgatgctt
     3541 cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc
     3601 tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatgctta
     3661 ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa
     3721 gcatcgtgta gtgtttttct tagtccgtta cgtaggtagg aatctgatgt aatggttgtt
     3781 ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt
     3841 tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc
     3901 accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg
     3961 ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca
     4021 aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac
     4081 tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag attatatttt
     4141 atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat
     4201 ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa
     4261 ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca
     4321 taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc
     4381 gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa
     4441 attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg
     4501 aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc actataccaa
     4561 ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt
     4621 aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc
     4681 gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag
     4741 aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc actactttag
     4801 tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga
     4861 ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt
     4921 ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc
     4981 tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat
     5041 tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt
     5101 tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc
     5161 tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg
     5221 cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg
     5281 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     5341 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag
     5401 tcccgcttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5521 tggccccagt gctgcaatga taccgcggct gccacgctca ccggctccag atttatcagc
     5581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6121 gagatccagt tcgatataac ccactcgtgt acccaactga tcttcagcat cttttacttt
     6181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6241 ggcgacacgg aaatgttgaa tactcat
//
