pJEB403+drrA vector (V005351)

LOCUS       V005351                 6945 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005351
VERSION     V005351
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Murry JP, Pandey AK, Sassetti CM, Rubin EJ.
  TITLE     Phthiocerol Dimycocerosate Transport Is Required for Resisting
            Interferon-gamma-Independent Immunity
  JOURNAL   J. Infect. Dis. 200 (5), 774-782 (2009)
   PUBMED   19622047
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Murry JP, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2008) Microbiology and Molecular Genetics, Harvard
            Medical School, 200 Longwood Ave., Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 6945)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Infect.
            Dis."; date: "2009"; volume: "200"; issue: "5"; pages: "774-782"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-AUG-2008) Microbiology and Molecular Genetics, Harvard Medical
            School, 200 Longwood Ave., Boston, MA 02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    218..477
                     /label="attP"
                     /note="attP"
     CDS             512..1624
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     primer_bind     2711..2727
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2736..2769
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     CDS             complement(2810..3466)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3467..3569)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(3570..4565)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     protein_bind    complement(4686..4719)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4775..5363
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5556..6548)
                     /gene="drrA"
                     /label="Doxorubicin resistance ATP-binding protein DrrA"
                     /note="Doxorubicin resistance ATP-binding protein DrrA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WQL9"
     protein_bind    complement(6588..6607)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     promoter        complement(6613..6643)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      complement(6689..6716)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6808..6894)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"