LOCUS       V005351                 6945 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005351
VERSION     V005351
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Murry JP, Pandey AK, Sassetti CM, Rubin EJ.
  TITLE     Phthiocerol Dimycocerosate Transport Is Required for Resisting
            Interferon-gamma-Independent Immunity
  JOURNAL   J. Infect. Dis. 200 (5), 774-782 (2009)
   PUBMED   19622047
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Murry JP, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2008) Microbiology and Molecular Genetics, Harvard
            Medical School, 200 Longwood Ave., Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 6945)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Infect.
            Dis."; date: "2009"; volume: "200"; issue: "5"; pages: "774-782"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-AUG-2008) Microbiology and Molecular Genetics, Harvard Medical
            School, 200 Longwood Ave., Boston, MA 02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    218..477
                     /label="attP"
                     /note="attP"
     CDS             512..1624
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     primer_bind     2711..2727
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    2736..2769
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     CDS             complement(2810..3466)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3467..3569)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(3570..4565)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     protein_bind    complement(4686..4719)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4775..5363
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5556..6548)
                     /gene="drrA"
                     /label="Doxorubicin resistance ATP-binding protein DrrA"
                     /note="Doxorubicin resistance ATP-binding protein DrrA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WQL9"
     protein_bind    complement(6588..6607)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     promoter        complement(6613..6643)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      complement(6689..6716)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6808..6894)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 cgctagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc
       61 tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag ctccaatctg
      121 gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg tcagcatcga
      181 aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa
      241 gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc tgaccagcgg
      301 agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca
      361 catatatggg ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa
      421 agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg
      481 gtagccttgt tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa
      541 gactcagcgc agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta
      601 ctacgcgctg cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa
      661 gcggctcatc gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc
      721 cagcgccatc acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga
      781 cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga
      841 agtggcggtc acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag
      901 gaagcacccg actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc
      961 ggtcgaggac aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga
     1021 gcgcgacgta gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga
     1081 gcactaccgg atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat
     1141 cgagcttcgc cgcaaggaca tcgtgcacga cggcatgacg atgaagctcc gggtgcgccg
     1201 tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa
     1261 gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg
     1321 tacgaagatg aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct
     1381 gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga
     1441 actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac
     1501 gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca
     1561 gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac
     1621 ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct
     1681 tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag
     1741 acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccagc tagaaggtac
     1801 atcttcgcaa gccggtgtaa cgatcttgag gctgtctaag aaaggagagt cgtgacgatg
     1861 aaacccgagg tcaacgtgta acagaaggcg acctaagtga tacctgtcac aaggtttgct
     1921 accgagtggg gcaggccgct acatttacga ccgcgtaacg ccagtcgatc ccacgcccag
     1981 tgggagacgg ccacggcgtc ggggaacaca actgaatatg gttccgcaga cgcaactaaa
     2041 ttaggggtat ccttgacagg caccaacatg tctccgtaat cggcggagac gcacgcacct
     2101 ttctcatgga gggaaacatg agcggcaaaa tccaacacaa agcagtggtt ccggccccta
     2161 gccgaatacc actcactctc agcgagatcg aagatcttcg caggaagggg tttaatcaga
     2221 ccgaaatcgc agagctgtat ggcgtgactc gacaagcggt gtcgtggcac aagaagacct
     2281 acggaggacg gttgaccacc aggcagatcg tccagcagaa ctggccgtgg gacacgcgga
     2341 aacctcacga taagtcgaag gcttttcaga ggctccggga tcacggcgag tacatgcgag
     2401 tgggtagctt ccgcacgatg tccgaagaca agaagaagcg cctattgtcg tggtggaaga
     2461 tgctccgcga cgacgactta gtgctcgagt tcgacccatc catcgagccc tacgaaggta
     2521 tggccggcgg cgggttcagg tacgtccccc gaggcatcga ggatgacgat ctcctgatcc
     2581 gggtgaacga gcacaccaac ctcaccgccg agggtgaact cctctggtcg tggccagacg
     2641 acatcgagga gcttctctcg gagccctaaa agtcacgacc ggttgtgtga gctagtaccg
     2701 ggccccccct cgaggtcgac ggtatcgata agcttataac ttcgtatagc atacattata
     2761 cgaagttatg ccaggagcat tgccgttccc gccagctaga ggggcgttac gccccgccct
     2821 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2881 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2941 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     3001 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     3061 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     3121 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     3181 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     3241 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     3301 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     3361 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     3421 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     3481 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     3541 gaaagttgga acctcttacg tgccgatcag gcgccggggg cggtgtccgg cggcccccag
     3601 aggaactgcg ccagttcctc cggatcggtg aagccggaga gatccagcgg ggtctcctcg
     3661 aacacctcga agtcgtgcag gaaggtgaag gcgagcagtt cgcgggcgaa gtcctcggtc
     3721 cgcttccact gcgccccgtc gagcagcgcg gccaggatct cgcggtcgcc ccggaaggcg
     3781 ttgagatgca gttgcaccag gctgtagcgg gagtctcccg catagacgtc ggtgaagtcg
     3841 acgatcccgg tgacctcggt cgcggccagg tccacgaaga tgttggtccc gtgcaggtcg
     3901 ccgtggacga accggggttc gcggccggcc agcagcgtgt ccacgtccgg cagccagtcc
     3961 tccaggcggt ccagcagccg gggcgagagg tagccccacc cgcggtggtc ctcgacggtc
     4021 gccgcgcggc gttcccgcag cagttccggg aagacctcgg aatggggggt gagcacggtg
     4081 ttcccggtca gcggcaccct gtgcagccgg ccgagcaccc ggccgagttc gcgggccagg
     4141 gcgagcagcg cgttccggtc ggtcgtgccg tccatcgcgg accgccaggt ggtgccggtc
     4201 atccggctca tcaccaggta gggccacggc caggctccgg tgccgggccg cagctcgccg
     4261 cggccgagga ggcggggcac cggcaccggg gcgtccgcca ggaccgcgta cgcctccgac
     4321 tccgacgcga ggctctccgg accgcaccag tgctcgccga acagcttgat caccgggtcg
     4381 ggctcgccga ccagtacggg gttggtgctc tcgccgggca cccgcagcac cggcggcacc
     4441 ggcagcccga gctcctccag ggctcggcgg gccagcggct cccagaattc ctggtcgttc
     4501 cgcaggctcg cgtaggaatc atccgaatca atacggtcga gaagtaacag ggattcttgt
     4561 gtcacagcgg acctctattc acagggtacg ggccggctta attccgcacg gccggtcgcg
     4621 acacggcctg tccgcaccgc ggtcaggcgt tgacgatgac gggctggtcg gccacgtcgg
     4681 ggagtataac ttcgtatagc atacattata cgaagttatg gatccactag ttccactgag
     4741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4921 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5161 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5461 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg
     5521 cgtgcggccg cggtacccta tggccgggcc gctcatcgcg cggaccccga caccagatgg
     5581 gtcagagact cggtgggatc ggtcgtcatg gccaggaata cgtgatcgag tgacggtcgg
     5641 cgcagcgcaa tatcggctag ctcgatcctc gcctcgtcga tccggcgcgc tgcctcgacg
     5701 agcatacgta tgccgtcagg cgccggcatc gtaatgcggt ctgagtcggg cgtcagcatc
     5761 gccctgtggt gctcgggcaa cagcgaaccg agcgccgcga cgatagcgtc cagatccttc
     5821 agatcgcggg gcactatttc gcagaaggtg tcgccggcgc ggtgcttgag ttcattcgcg
     5881 gtgccttcgg cgatgattat gccgtgatcg atcaggatga tgcggtcact gagcgcatcc
     5941 gcctcctcga gatactgcgt ggtcaacaac gtggcaatgc ccagcttctt gaagctggcc
     6001 accagatccc aaatagcttg ccggctcctg ggatcgagcc cggtggtggg ctcgtctaag
     6061 aacgccacct ggggttggac caccaatccg cacgcgatgt ctattcgtcg gcgcattccg
     6121 ccggagtagg tgcccacccg cctctttccg gcatgtacga ggctgaattg ctcgagcagt
     6181 tcggcggcgc gtttgcgcgc cgcggacttg ctcagtcccc acagacgacc aaacaacacc
     6241 aggttctgct caccggaaag cgcgtcgtcg acggccacct gctgcccggt gaccatgatc
     6301 gagcggcgta caccggccgg ttcggaaaca acatcgtagc cagcgatgat cgccgagccg
     6361 gcatccggtc gggtcagcgt cgacaagatg tccaccatgg tcgtcttgcc ggccccgttg
     6421 gggcccagca gcccgatcac ttcaccgcgg cgcaccttga aactcacgtc atcgagggcc
     6481 acaatcttgc ccttgccgta ggtcttgcga accccgttaa ccaccaccgc catgtcgtcg
     6541 ttgcgcatcc gatcctcctc agccttcgaa ttctgcagct ggatccgaat tgtgagcgct
     6601 cacaattcca cacattatac gagccggaag cataaagtgt caagcctggg gtctagatat
     6661 gacgacagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     6721 aatttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     6781 cgcaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     6841 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     6901 ggcagttccc tactctcgca tggggagacc ccacactacc atcgg
//
