pJBtetT7POL vector (V005361)

LOCUS       V005361                13318 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005361
VERSION     V005361
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(796..1983)
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(2031..2059)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(2072..2088)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2699..3844)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      complement(3953..4002)
                     /label="neo"
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(4236..4766)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     CDS             complement(5875..8523)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      complement(8552..8736)
                     /label="LLT"
                     /note="LLT"
                     /regulatory_class="terminator"
     regulatory      complement(8847..8928)
                     /label="fdT"
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      8877..8925
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(9104..9152)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(9802..9867)
                     /label="lambda N peptide"
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et
                     al., 2004)"
     misc_feature    complement(10019..10089)
                     /label="nutL"
                     /note="nutL"
     protein_bind    10148..10164
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10172..10216)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        10558..10635
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             10636..11715
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(12752..12856)
                     /label="AmpR promoter"
     oriT            13033..13141
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     complement(13228..13244)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"