LOCUS       V005361                13318 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005361
VERSION     V005361
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13318)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(796..1983)
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(2031..2059)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(2072..2088)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2699..3844)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      complement(3953..4002)
                     /label="neo"
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(4236..4766)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     CDS             complement(5875..8523)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      complement(8552..8736)
                     /label="LLT"
                     /note="LLT"
                     /regulatory_class="terminator"
     regulatory      complement(8847..8928)
                     /label="fdT"
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      8877..8925
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(9104..9152)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(9802..9867)
                     /label="lambda N peptide"
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et
                     al., 2004)"
     misc_feature    complement(10019..10089)
                     /label="nutL"
                     /note="nutL"
     protein_bind    10148..10164
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10172..10216)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        10558..10635
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             10636..11715
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(12752..12856)
                     /label="AmpR promoter"
     oriT            13033..13141
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     complement(13228..13244)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
       61 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
      121 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
      181 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
      241 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
      301 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
      361 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
      421 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
      481 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
      541 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
      601 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc
      661 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
      721 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
      781 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
      841 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga
      901 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc
      961 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     1021 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     1081 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc
     1141 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     1201 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     1261 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     1321 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     1381 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg
     1441 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     1501 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     1561 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     1621 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     1681 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
     1741 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
     1801 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
     1861 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
     1921 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
     1981 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
     2041 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact
     2101 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2161 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2221 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2281 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc
     2701 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     2761 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     2821 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     2881 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     2941 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     3001 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     3061 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     3121 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     3181 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     3241 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     3301 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     3361 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     3421 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     3481 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     3541 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     3601 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     3661 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
     3721 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     3781 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     3841 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct
     3901 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     3961 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     4021 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     4081 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4141 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga
     4201 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg
     4261 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca
     4321 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     4381 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     4441 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     4501 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     4561 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     4621 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     4681 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
     4741 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct
     4801 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtagct ttcctgacgg
     5581 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag
     5641 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt
     5701 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg
     5761 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc tgatcggcaa ggtgttctgg
     5821 tcggcggggg gggggggggg ggggggatcc cggagtcgta ttgatttggc gttacgcgaa
     5881 cgcgaagtcc gactctaaga tgtcacggag gttcaagtta cctttagccg gaagtgctgg
     5941 cattttgtcc aattgagact cgtgcaactg gtcagcgaac tggtcgtaga aatcagccag
     6001 tacatcacaa gactcatatg tgtcaaccat agtttcgcgc actgctttga acaggttcgc
     6061 agcgtcagcc ggaatggtac cgaaggagtc gtgaatcagt gcaaaagatt cgattccgta
     6121 cttctcgtgt gcccacacta cagtcttacg aaggtggcta ccgtcttggc tgtgtacaaa
     6181 gttaggagcg ataccagact cctgtttgtg tgcatcaatc tcgctatctt tgttggtgtt
     6241 aatggtaggc tgtaagcgga actgaccgag gaacatcagg ttcaagcgcg tctgaatagg
     6301 cttcttgtat tcctgccaca cagggaaacc atcaggagtt acccaatgca cagcgcaacg
     6361 cttgcgaaga atctctccag tcttcttatc tttgacctca gcagccagca gcttagcagc
     6421 agacttaagc cagttcattg cttcaaccgc agctaccacc gtcacgctca cagattccca
     6481 aatcagctta gccatgtatc cagcagcctg attcggctga gtgaacatca gacccttgcc
     6541 ggaatcaata gctggctgaa tggtatcttc cagcacttgt tgacggaagc cgaactcttt
     6601 ggacccgtaa gccagcgtca tgactgaacg cttagtcaca ctgcgagtaa caccgtaagc
     6661 cagccattga ccagccagtg ccttagtgcc cagcttgact ttctcagaga tttcaccagt
     6721 gttctcatcg gtcacggtaa ctacttcgtt atcggtccca ttgattgcgt ctgcttgtag
     6781 aatctcgttg actttcttag caacaatccc gtagatgtcc tgaacggttt cactaggaag
     6841 caagttaacc gcgcgaccac ctacctcatc tcggagcatc gcggagaagt gctggatgcc
     6901 agagcaagac ccgtcaaacg ccagcggaag ggagcagtta tagctcaggc cgtggtgctg
     6961 taccccagcg tactcaaagc agaacgcaag gaagcagaac ggagaatctt gctcagccca
     7021 ccaagtgttc tccagtggag acttagcgca agccatgatg ttctcgtggt tttcctcaat
     7081 gaacttgatg cgctcaggga acggaacctt atcgacaccc gcacagtttg caccgtggat
     7141 tttcagccag tagtaacctt ccttaccgat tggtttacct ttcgccagcg taagcagtcc
     7201 tttggtcata tcgttacctt gcgggttgaa cattgacaca gcgtaaacac gaccgcgcca
     7261 gtccatgttg taagggaacc agatggcctt atggttagca aacttattgg cttgctcaag
     7321 catgaactca aggctgatac ggcgagactt gcgagccttg tccttgcggt acacagcagc
     7381 ggcagcacgt ttccacgcgg tgagagcctc aggattcatg tcgatgtctt ccggtttcat
     7441 cgggagttct tcacgctcaa tcgcagggat gtcctcgacc ggacaatgct tccacttggt
     7501 gattacgttg gcgaccgcta ggactttctt gttgattttc catgcggtgt tttgcgcaat
     7561 gttaatcgct ttgtacacct caggcatgta aacgtcttcg tagcgcatca gtgctttctt
     7621 actgtgagta cgcaccagcg ccagaggacg acgaccgtta gcccaatagc caccaccagt
     7681 aatgccagtc cacggcttag gaggaactac gcaaggttgg aacatcggag agatgccagc
     7741 cagcgcacct gcacgggttg cgatagcctc agcgtattca ggtgcgagtt cgatagtctc
     7801 agagtcttga cctactacgc cagcattttg gcggtgtaag ctaaccattc cggttgactc
     7861 aatgagcatc tcgatgcagc gtactcctac atgaatagag tcttccttat gccacgaaga
     7921 ccacgcctcg ccaccgagta gacccttaga gagcatgtca gcctcgacaa cttgcataaa
     7981 tgctttcttg tagacgtgcc ctacgcgctt gttgagttgt tcctcaacgt ttttcttgaa
     8041 gtgcttagct tcaaggtcac ggatacgacc gaagcgagcc tcgtcctcaa tggcccgacc
     8101 gattgcgctt gctacagcct gaacggttgt attgtcagca ctggttaggc aagccagagt
     8161 ggtcttaatg gtgatgtacg ctacggcttc cggcttgatt tcttgcagga actggaaggc
     8221 tgtcgggcgc ttgccgcgct tagctttcac ttcctcaaac cagtcgttga tgcgtgcaat
     8281 catcttaggg agtagggtag tgatgagagg cttggcggca gcgttatccg caacctcacc
     8341 agctttaagt tgacgctcaa acatcttgcg gaagcgtgct tcacccatct cgtaagactc
     8401 atgctcaagg gccaactgtt cgcgagctaa acgctcaccg taatggtcag ccagagtgtt
     8461 gaacgggata gcagccagtt cgatgtcaga gaagtcgttc ttagcgatgt taatcgtgtt
     8521 catttagtgc ctcttccagt tagtaaatcc gggatccact cgttattctc ggacgagtgt
     8581 tcagtaatga acctctggag agaaccatgt atatgatcgt tatctgggtt ggacttctgc
     8641 ttttaagccc agataactgg cctgaatatg ttaatgagag aatcggtatt cctcatgtgt
     8701 ggcatgtttt cgtctttgct cttgcatttt cgctagaggt cgaaattctg tgtgaaattg
     8761 ttatccgctc agaattttct gtatgaggtt ttgctaaaca actttcaaca gtttcagcgg
     8821 agtgagaata gaaaggaaca actaaaggaa ttgcgaataa taattttttc acgttgaaaa
     8881 tctccaaaaa aaaaggctcc aaaaggagcc tttaattgta tcggtttatc agcttgcttt
     8941 cgaggtgaat ttcgaccctc tagaggtcga aattctgtgt gaaattgtta tccgctcaga
     9001 attttctgta tgaggttttg ctaaacaact ttcaacagtt tcagcggagt gagaatagaa
     9061 aggaacaact aaaggaattg cgaataataa ttttttcacg ttgaaaatct ccaaaaaaaa
     9121 aggctccaaa aggagccttt aattgtatcg gtttatcagc ttgctttcga ggtgaatttc
     9181 gaccctctag caattaatgt gcatcgatta tcagctattg ccagcgccag atataagcga
     9241 tttaagctaa gaaaacgcat taagatgcaa aacgataaag tgcgatcagt aattcaaaac
     9301 cttacagaag agcaatctat ggttttgtgc gcagccctta atgaaggcag gaagtatgtg
     9361 gttacatcaa aacaattccc atacattagt gagttgattg agcttggtgt gttgaacaaa
     9421 actttttccc gatggaatgg aaagcatata ttattcccta ttgaggatat ttactggact
     9481 gaattagttg ccagctatga tccatataat attgagataa agccaaggcc aatatctaag
     9541 taactagata agaggaatcg attttccctt aattttctgg cgtccactgc atgttatgcc
     9601 gcgttcgcca ggcttgctgt accatgtgcg ctgattcttg cgctcaatac gttgcaggtt
     9661 gctttcaatc tgtttgtggt attcagccag cactgtaagg tctatcggat ttagtgcgct
     9721 ttctactcgt gatttcggtt tgcgattcag cgagagaata gggcggttaa ctggttttgc
     9781 gcttacccca accaacaggg gatttgctgc tttccattga gcctgtttct ctgcgcgacg
     9841 ttcgcggcgg cgtgtttgtg catccatctg gattctcctg tcagttagct ttggtggtgt
     9901 gtggcagttg tagtcctgaa cgaaaacccc ccgcgattgg cacattggca gctaatccgg
     9961 aatcgcactt acggccaatg cttcgtttcg tatcacacac cccaaagcct tctgctttga
    10021 atgctgccct tcttcagggc ttaattttta agagcgtcac cttcatggtg gtcagtgcgt
    10081 cctgctgatg gggttataat tacctcaggt cgacgtccca tgtgcaggtg ctgaattctg
    10141 tgtgaaattg ttatccgctc acaattgaat ctattataat tgttatccgc tcacaaagca
    10201 aataaatttt ttatgatttc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
    10261 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
    10321 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
    10381 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
    10441 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
    10501 ggcatggcgg ccggtcgaca attcgcgcgc gaaggcgaag cggcatgcat ttacgttgac
    10561 accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
    10621 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
    10681 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
    10741 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
    10801 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
    10861 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
    10921 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
    10981 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
    11041 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
    11101 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
    11161 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
    11221 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
    11281 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
    11341 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
    11401 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
    11461 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
    11521 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
    11581 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
    11641 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagcgcgaat tgtcgactct
    11761 agaggatcgg gggggggggg aaagccacgt tgtgtctcaa aatctctgat gttacattgc
    11821 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
    11881 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
    11941 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
    12001 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
    12061 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
    12121 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
    12181 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
    12241 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
    12301 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
    12361 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
    12421 agtctggaaa gaaatgcata agctactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12481 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
    12541 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12601 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12661 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12721 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12781 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12841 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgatc gacggatctt
    12901 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
    12961 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
    13021 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
    13081 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
    13141 gtaagctcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    13201 ggccctttcg tcttcaagaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga
    13261 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagcaga
//
